H2_VACCW
ID H2_VACCW Reviewed; 189 AA.
AC P08583;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 2.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Protein H2;
GN OrderedLocusNames=VACWR100; ORFNames=H2R;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3021979; DOI=10.1128/jvi.60.2.436-449.1986;
RA Rosel J.L., Earl P.L., Weir J.P., Moss B.;
RT "Conserved TAAATG sequence at the transcriptional and translational
RT initiation sites of vaccinia virus late genes deduced by structural and
RT functional analysis of the HindIII H genome fragment.";
RL J. Virol. 60:436-449(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
RX PubMed=3517852; DOI=10.1073/pnas.83.10.3141;
RA Broyles S.S., Moss B.;
RT "Homology between RNA polymerases of poxviruses, prokaryotes, and
RT eukaryotes: nucleotide sequence and transcriptional analysis of vaccinia
RT virus genes encoding 147-kDa and 22-kDa subunits.";
RL Proc. Natl. Acad. Sci. U.S.A. 83:3141-3145(1986).
RN [4]
RP FUNCTION.
RX PubMed=15795260; DOI=10.1128/jvi.79.8.4744-4754.2005;
RA Senkevich T.G., Moss B.;
RT "Vaccinia virus H2 protein is an essential component of a complex involved
RT in virus entry and cell-cell fusion.";
RL J. Virol. 79:4744-4754(2005).
RN [5]
RP IDENTIFICATION IN A COMPLEX WITH A16; A21; A28; G3; G9; J5 AND L5.
RX PubMed=16339313; DOI=10.1073/pnas.0509239102;
RA Senkevich T.G., Ojeda S., Townsley A., Nelson G.E., Moss B.;
RT "Poxvirus multiprotein entry-fusion complex.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:18572-18577(2005).
CC -!- FUNCTION: Envelope protein part of the entry-fusion complex responsible
CC for the virus membrane fusion with host cell membrane during virus
CC entry. Also plays a role in cell-cell fusion (syncytium formation)
CC (Probable). {ECO:0000305|PubMed:15795260}.
CC -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at least
CC composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation
CC of the viral membrane is necessary for the assembly of the complex.
CC {ECO:0000269|PubMed:16339313}.
CC -!- INTERACTION:
CC P08583; P68633: VACWR151; NbExp=3; IntAct=EBI-7564589, EBI-6969231;
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC III membrane protein {ECO:0000305}. Note=Component of the mature virion
CC (MV) membrane (By similarity). The mature virion is located in the
CC cytoplasm of infected cells and is probably released by cell lysis.
CC {ECO:0000250}.
CC -!- PTM: Contains two intramolecular disulfide bonds. They are created by
CC the viral disulfide bond formation pathway, a poxvirus-specific pathway
CC that operates on the cytoplasmic side of the MV membranes (Probable).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the poxviridae H2 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M13209; AAB59837.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89379.1; -; Genomic_DNA.
DR PIR; B24481; QQVZH2.
DR RefSeq; YP_232982.1; NC_006998.1.
DR SMR; P08583; -.
DR IntAct; P08583; 1.
DR MINT; P08583; -.
DR TCDB; 1.G.11.1.1; the poxvirus cell entry protein complex (pep-c) family.
DR DNASU; 3707556; -.
DR GeneID; 3707556; -.
DR KEGG; vg:3707556; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR InterPro; IPR005023; Pox_LP_H2.
DR Pfam; PF03356; Pox_LP_H2; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Fusion of virus membrane with host cell membrane;
KW Fusion of virus membrane with host membrane; Late protein; Membrane;
KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix;
KW Viral envelope protein; Viral penetration into host cytoplasm; Virion;
KW Virus entry into host cell.
FT CHAIN 1..189
FT /note="Protein H2"
FT /id="PRO_0000099548"
FT TOPO_DOM 1..28
FT /note="Intravirion"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..49
FT /note="Helical; Signal-anchor for type III membrane
FT protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 50..189
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT CONFLICT 77
FT /note="D -> V (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 121
FT /note="P -> A (in Ref. 1; AAB59837)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 189 AA; 21543 MW; FFE12787A38C4794 CRC64;
MDKTTLSVNA CNLEYVREKA IVGVQAAKTS TLIFFVIILA ISALLLWFQT SDNPVFNELT
RYMRIKNTVN DWKSLTDSKT KLESDRGRLL AAGKDDIFEF KCVDFGAYFI AMRLDKKTYL
PQAIRRGTGD AWMVKKAAKV DPSAQQFCQY LIKHKSNNVI TCGNEMLNEL GYSGYFMSPH
WCSDFSNME
