ID   H2_VARV                 Reviewed;         189 AA.
AC   P0DSZ0; P33061;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Late protein H2;
GN   ORFNames=H2R, I2R;
OS   Variola virus.
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10255;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   PubMed=8264798; DOI=10.1038/366748a0;
RA   Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C.,
RA   Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A.,
RA   Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.;
RT   "Potential virulence determinants in terminal regions of variola smallpox
RT   virus genome.";
RL   Nature 366:748-751(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Garcia-1966;
RA   Shchelkunov S.N., Balkin I.V., Totmenin A.V., Resenchuk S.M., Blinov V.M.,
RA   Sandakhchiev L.S.;
RL   Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Envelope protein part of the entry-fusion complex responsible
CC       for the virus membrane fusion with host cell membrane during virus
CC       entry. Also plays a role in cell-cell fusion (syncytium formation) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at least
CC       composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation
CC       of the viral membrane is necessary for the assembly of the complex (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC       III membrane protein {ECO:0000305}. Note=Component of the mature virion
CC       (MV) membrane. The mature virion is located in the cytoplasm of
CC       infected cells and is probably released by cell lysis. {ECO:0000250}.
CC   -!- PTM: Contains two intramolecular disulfide bonds. They are created by
CC       the viral disulfide bond formation pathway, a poxvirus-specific pathway
CC       that operates on the cytoplasmic side of the MV membranes (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae H2 family. {ECO:0000305}.
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DR   EMBL; L22579; AAA60833.1; -; Genomic_DNA.
DR   EMBL; X76264; CAA53839.1; -; Genomic_DNA.
DR   PIR; C72161; C72161.
DR   PIR; T28523; T28523.
DR   RefSeq; NP_042129.1; NC_001611.1.
DR   SMR; P0DSZ0; -.
DR   GeneID; 1486440; -.
DR   KEGG; vg:1486440; -.
DR   Proteomes; UP000119805; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR005023; Pox_LP_H2.
DR   Pfam; PF03356; Pox_LP_H2; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Fusion of virus membrane with host cell membrane;
KW   Fusion of virus membrane with host membrane; Late protein; Membrane;
KW   Signal-anchor; Transmembrane; Transmembrane helix; Viral envelope protein;
KW   Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT   CHAIN           1..189
FT                   /note="Late protein H2"
FT                   /id="PRO_0000448197"
FT   TOPO_DOM        1..28
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        29..49
FT                   /note="Helical; Signal-anchor for type III membrane
FT                   protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        50..189
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   189 AA;  21509 MW;  FFE12787AD6C4794 CRC64;
     MDKTTLSVNA CNLEYVREKA IVGVQAAKTS TLIFFVIILA ISALLLWFQT SDNPVFNELT
     RYMRIKNTVN DWKSLTDSKT KLESDRGRLL AAGKDDIFEF KCVDFGAYFI AMRLDKKTYL
     PQAIRRGTGD AWMVKKAAKV DPSAQQFCQY LIKHKSNNVI TCGNEMLNEL GYSGYFMSPH
     WCSDLSNME