ID   H33_TETPY               Reviewed;         136 AA.
AC   P15512;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Histone H3.3;
DE   AltName: Full=H3.2;
DE   AltName: Full=Minor histone H3 variant;
DE   AltName: Full=hv2;
OS   Tetrahymena pyriformis.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC   Tetrahymena.
OX   NCBI_TaxID=5908;
RN   [1]
RP   PROTEIN SEQUENCE OF 2-136.
RX   PubMed=6432775; DOI=10.1093/oxfordjournals.jbchem.a134788;
RA   Hayashi T., Hayashi H., Fusauchi Y., Iwai K.;
RT   "Tetrahymena histone H3. Purification and two variant sequences.";
RL   J. Biochem. 95:1741-1749(1984).
CC   -!- FUNCTION: Macronuclear replacement variant which replaces conventional
CC       H3 in a subset of nucleosomes. Nucleosomes wrap and compact DNA into
CC       chromatin, limiting DNA accessibility to the cellular machineries which
CC       require DNA as a template. Histones thereby play a central role in
CC       transcription regulation, DNA repair, DNA replication and chromosomal
CC       stability. DNA accessibility is regulated via a complex set of post-
CC       translational modifications of histones, also called histone code, and
CC       nucleosome remodeling. Functions redundantly to H3.4. H3.3 deposition
CC       into chromatin is mainly transcription-associated and DNA replication-
CC       independent, but it can also enter a replication-coupled pathway.
CC       Although not essential for vegetative growth, minor H3 variants are
CC       required for producing viable conjugation progeny by affecting late
CC       developmental stages of conjugation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: The nucleosome is a histone octamer containing two molecules
CC       each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and
CC       two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of
CC       DNA.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Chromosome {ECO:0000250}. Note=Localizes
CC       mainly to the large, transcriptionally active, somatic macronucleus
CC       (MAC) and only faintly to the small, transcriptionally inert, germ line
CC       micronucleus (MIC). {ECO:0000250}.
CC   -!- PTM: Acetylated. Acetylation occurs almost exclusively in the MAC (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the histone H3 family. {ECO:0000305}.
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DR   PIR; B28852; B28852.
DR   AlphaFoldDB; P15512; -.
DR   SMR; P15512; -.
DR   GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR   GO; GO:0030527; F:structural constituent of chromatin; IEA:InterPro.
DR   Gene3D; 1.10.20.10; -; 1.
DR   InterPro; IPR009072; Histone-fold.
DR   InterPro; IPR007125; Histone_H2A/H2B/H3.
DR   InterPro; IPR000164; Histone_H3/CENP-A.
DR   PANTHER; PTHR11426; PTHR11426; 1.
DR   Pfam; PF00125; Histone; 1.
DR   PRINTS; PR00622; HISTONEH3.
DR   SMART; SM00428; H3; 1.
DR   SUPFAM; SSF47113; SSF47113; 1.
DR   PROSITE; PS00322; HISTONE_H3_1; 1.
DR   PROSITE; PS00959; HISTONE_H3_2; 1.
PE   1: Evidence at protein level;
KW   Chromosome; Direct protein sequencing; DNA-binding; Nucleosome core;
KW   Nucleus.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:6432775"
FT   CHAIN           2..136
FT                   /note="Histone H3.3"
FT                   /id="PRO_0000221347"
FT   REGION          1..41
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   136 AA;  15519 MW;  FD4C663F394A6065 CRC64;
     MARTKQTARK STGVKAPRKQ LATKAARKSA PVSGGVKKPH KFRPGTVALR EIRKYQKTTD
     LLIRKLPFQR LVRDIAMEMK NDIRFQSQAI LALQEAAEAY LVGLFEDTNL CAIHARRVTI
     MTKDMQLARR IRGERF