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H3H_NEONM
ID   H3H_NEONM               Reviewed;         422 AA.
AC   A0A3G9K5C8;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   13-FEB-2019, sequence version 1.
DT   03-AUG-2022, entry version 9.
DE   RecName: Full=Hispidin-3-hydroxylase {ECO:0000303|PubMed:30478037};
DE            Short=H3H {ECO:0000303|PubMed:30478037};
DE            EC=1.-.-.- {ECO:0000269|PubMed:26094784};
DE   AltName: Full=FAD-dependent monooxygenase h3h {ECO:0000303|PubMed:30478037};
DE   AltName: Full=Fungal bioluminescence cycle protein h3h {ECO:0000303|PubMed:30478037};
GN   Name=h3h {ECO:0000303|PubMed:30478037};
OS   Neonothopanus nambi (Agaricus nambi).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Omphalotaceae; Neonothopanus.
OX   NCBI_TaxID=71958;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION, FUNCTION, PATHWAY, AND
RP   BIOTECHNOLOGY.
RX   PubMed=30478037; DOI=10.1073/pnas.1803615115;
RA   Kotlobay A.A., Sarkisyan K.S., Mokrushina Y.A., Marcet-Houben M.,
RA   Serebrovskaya E.O., Markina N.M., Gonzalez Somermeyer L.,
RA   Gorokhovatsky A.Y., Vvedensky A., Purtov K.V., Petushkov V.N.,
RA   Rodionova N.S., Chepurnyh T.V., Fakhranurova L.I., Guglya E.B.,
RA   Ziganshin R., Tsarkova A.S., Kaskova Z.M., Shender V., Abakumov M.,
RA   Abakumova T.O., Povolotskaya I.S., Eroshkin F.M., Zaraisky A.G.,
RA   Mishin A.S., Dolgov S.V., Mitiouchkina T.Y., Kopantzev E.P.,
RA   Waldenmaier H.E., Oliveira A.G., Oba Y., Barsova E., Bogdanova E.A.,
RA   Gabaldon T., Stevani C.V., Lukyanov S., Smirnov I.V., Gitelson J.I.,
RA   Kondrashov F.A., Yampolsky I.V.;
RT   "Genetically encodable bioluminescent system from fungi.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:12728-12732(2018).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=26094784; DOI=10.1002/anie.201501779;
RA   Purtov K.V., Petushkov V.N., Baranov M.S., Mineev K.S., Rodionova N.S.,
RA   Kaskova Z.M., Tsarkova A.S., Petunin A.I., Bondar V.S., Rodicheva E.K.,
RA   Medvedeva S.E., Oba Y., Oba Y., Arseniev A.S., Lukyanov S., Gitelson J.I.,
RA   Yampolsky I.V.;
RT   "The chemical basis of fungal bioluminescence.";
RL   Angew. Chem. Int. Ed. 54:8124-8128(2015).
CC   -!- FUNCTION: Hispidin-3-hydroxylase; part of the gene cluster that
CC       mediates the fungal bioluminescence cycle (PubMed:30478037,
CC       PubMed:26094784). Hydroxylates hispidin in order to produce the fungal
CC       luciferin 3-hydroxyhispidin (PubMed:30478037, PubMed:26094784). The
CC       fungal bioluminescence cycle begins with the hispidin synthetase that
CC       catalyzes the formation of hispidin which is further hydroxylated by
CC       the hispidin-3-hydroxylase, yielding the fungal luciferin 3-
CC       hydroxyhispidin. The luciferase then produces an endoperoxide as a
CC       high-energy intermediate with decomposition that yields oxyluciferin
CC       (also known as caffeoylpyruvate) and light emission. Oxyluciferin can
CC       be recycled to caffeic acid by caffeoylpyruvate hydrolase
CC       (PubMed:30478037) (Probable). {ECO:0000269|PubMed:26094784,
CC       ECO:0000269|PubMed:30478037, ECO:0000305|PubMed:30478037}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + hispidin + NADH + O2 = 3-hydroxyhispidin + H2O +
CC         NAD(+); Xref=Rhea:RHEA:71115, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:190288, ChEBI:CHEBI:190289;
CC         Evidence={ECO:0000250|UniProtKB:A0A146I0C4};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71116;
CC         Evidence={ECO:0000250|UniProtKB:A0A146I0C4};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + hispidin + NADPH + O2 = 3-hydroxyhispidin + H2O +
CC         NADP(+); Xref=Rhea:RHEA:71119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:190288, ChEBI:CHEBI:190289;
CC         Evidence={ECO:0000269|PubMed:26094784};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71120;
CC         Evidence={ECO:0000269|PubMed:26094784};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000250|UniProtKB:A6T923};
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:30478037}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:A0A146I0C4}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- BIOTECHNOLOGY: The availability of a complete eukaryotic luciferin
CC       biosynthesis pathway provides several applications in biomedicine and
CC       bioengineering. {ECO:0000269|PubMed:30478037}.
CC   -!- SIMILARITY: Belongs to the paxM FAD-dependent monooxygenase family.
CC       {ECO:0000269|PubMed:30478037, ECO:0000305}.
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DR   EMBL; LC435367; BBH43497.1; -; mRNA.
DR   SMR; A0A3G9K5C8; -.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002938; FAD-bd.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   1: Evidence at protein level;
KW   FAD; Flavoprotein; Membrane; Monooxygenase; Oxidoreductase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..422
FT                   /note="Hispidin-3-hydroxylase"
FT                   /id="PRO_0000455706"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         18
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A6T923"
FT   BINDING         37..38
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A6T923"
FT   BINDING         247..249
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:A6T923"
FT   BINDING         318
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A6T923"
FT   BINDING         328..332
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A6T923"
SQ   SEQUENCE   422 AA;  46449 MW;  3CE9DAD13C0E4A57 CRC64;
     MASFENSLSV LIVGAGLGGL AAAIALRRQG HVVKIYDSSS FKAELGAGLA VPPNTLRSLQ
     QLGCNTENLN GVDNLCFTAM GYDGSVGMMN NMTDYREAYG TSWIMVHRVD LHNELMRVAL
     DPGGLGPPAT LHLNHRVTFC DVDACTVTFT NGTTQSADLI VGADGIRSTI RRFVLEEDVT
     VPASGIVGFR WLVQADALDP YPELDWIVKK PPLGARLIST PQNPQSGVGL ADRRTIIIYA
     CRGGTMVNVL AVHDDERDQN TADWSVPASK DDLFRVFHDY HPRFRRLLEL AQDINLWQMR
     VVPVLKKWVN KRVCLLGDAA HASLPTLGQG FGMGLEDAVA LGTLLPKGTT ASQIETRLAV
     YEQLRKDRAE FVAAESYEEQ YVPEMRGLYL RSKELRDRVM GYDIKVESEK VLETLLRSSN
     SA
 
 
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