H3_VACCW
ID H3_VACCW Reviewed; 324 AA.
AC P07240; Q80HW3;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 02-JUN-2021, entry version 79.
DE RecName: Full=Envelope protein H3;
DE AltName: Full=Ag35;
DE AltName: Full=Virion envelope protein p35;
GN OrderedLocusNames=VACWR101; ORFNames=H3L;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3021979; DOI=10.1128/jvi.60.2.436-449.1986;
RA Rosel J.L., Earl P.L., Weir J.P., Moss B.;
RT "Conserved TAAATG sequence at the transcriptional and translational
RT initiation sites of vaccinia virus late genes deduced by structural and
RT functional analysis of the HindIII H genome fragment.";
RL J. Virol. 60:436-449(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RX PubMed=2462305;
RA Gordon J., Kovala T., Dales S.;
RT "Molecular characterization of a prominent antigen of the vaccinia virus
RT envelope.";
RL Virology 167:361-369(1988).
RN [4]
RP FUNCTION.
RX PubMed=10708453; DOI=10.1128/jvi.74.7.3353-3365.2000;
RA Lin C.L., Chung C.-S., Heine H.G., Chang W.;
RT "Vaccinia virus envelope H3L protein binds to cell surface heparan sulfate
RT and is important for intracellular mature virion morphogenesis and virus
RT infection in vitro and in vivo.";
RL J. Virol. 74:3353-3365(2000).
RN [5]
RP TOPOLOGY, SIGNAL-ANCHOR, INDUCTION, AND SUBCELLULAR LOCATION.
RX PubMed=10906204; DOI=10.1128/jvi.74.16.7508-7517.2000;
RA da Fonseca F.G., Wolffe E.J., Weisberg A., Moss B.;
RT "Characterization of the vaccinia virus H3L envelope protein: topology and
RT posttranslational membrane insertion via the C-terminal hydrophobic tail.";
RL J. Virol. 74:7508-7517(2000).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=10906205; DOI=10.1128/jvi.74.16.7518-7528.2000;
RA da Fonseca F.G., Wolffe E.J., Weisberg A., Moss B.;
RT "Effects of deletion or stringent repression of the H3L envelope gene on
RT vaccinia virus replication.";
RL J. Virol. 74:7518-7528(2000).
CC -!- FUNCTION: Envelope protein that binds to heparan sulfate on the cell
CC surface and might provide virion attachment to target cell.
CC {ECO:0000250, ECO:0000269|PubMed:10708453, ECO:0000269|PubMed:2462305}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000269|PubMed:10906204};
CC Single-pass membrane protein {ECO:0000269|PubMed:10906204}.
CC Note=Component of the mature virion (MV) membrane. Becomes membrane
CC associated presumably during virus maturation. The mature virion is
CC located in the cytoplasm of infected cells and is probably released by
CC cell lysis.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC {ECO:0000269|PubMed:10906204}.
CC -!- PTM: Does not contain disulfide bonds. {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Impaired virion assembly.
CC {ECO:0000269|PubMed:10906205}.
CC -!- MISCELLANEOUS: Immunodominant protein.
CC -!- SIMILARITY: Belongs to the chordopoxvirinae protein H3 family.
CC {ECO:0000305}.
CC -!- CAUTION: Was originally mixed up with the protein H5 encoded by encoded
CC by H5R gene. {ECO:0000305|PubMed:2462305}.
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DR EMBL; M13209; AAB59838.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89380.1; -; Genomic_DNA.
DR PIR; C24481; QQVZH3.
DR PDB; 5EJ0; X-ray; 1.90 A; A=4-240.
DR PDBsum; 5EJ0; -.
DR SMR; P07240; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR InterPro; IPR004900; Poxvirus_P35.
DR Pfam; PF03213; Pox_P35; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Host-virus interaction; Late protein; Membrane;
KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix;
KW Viral attachment to host cell; Viral envelope protein; Virion;
KW Virus entry into host cell.
FT CHAIN 1..324
FT /note="Envelope protein H3"
FT /id="PRO_0000099208"
FT TOPO_DOM 1..284
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical; Signal-anchor"
FT /evidence="ECO:0000305"
FT TOPO_DOM 306..324
FT /note="Intravirion"
FT /evidence="ECO:0000255"
FT CONFLICT 195
FT /note="E -> A (in Ref. 2; AAO89380)"
FT /evidence="ECO:0000305"
FT STRAND 6..11
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 13..16
FT /evidence="ECO:0007829|PDB:5EJ0"
FT HELIX 18..20
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 62..67
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:5EJ0"
FT HELIX 79..81
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 86..89
FT /evidence="ECO:0007829|PDB:5EJ0"
FT HELIX 92..105
FT /evidence="ECO:0007829|PDB:5EJ0"
FT HELIX 108..115
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 120..125
FT /evidence="ECO:0007829|PDB:5EJ0"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:5EJ0"
FT HELIX 136..145
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 150..152
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 164..169
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 172..178
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 186..191
FT /evidence="ECO:0007829|PDB:5EJ0"
FT HELIX 192..204
FT /evidence="ECO:0007829|PDB:5EJ0"
FT HELIX 212..222
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 227..230
FT /evidence="ECO:0007829|PDB:5EJ0"
FT HELIX 233..235
FT /evidence="ECO:0007829|PDB:5EJ0"
FT STRAND 237..239
FT /evidence="ECO:0007829|PDB:5EJ0"
SQ SEQUENCE 324 AA; 37504 MW; FF3734631040617F CRC64;
MAAAKTPVIV VPVIDRLPSE TFPNVHEHIN DQKFDDVKDN EVMPEKRNVV VVKDDPDHYK
DYAFIQWTGG NIRNDDKYTH FFSGFCNTMC TEETKRNIAR HLALWDSNFF TELENKKVEY
VVIVENDNVI EDITFLRPVL KAMHDKKIDI LQMREIITGN KVKTELVMDK NHAIFTYTGG
YDVSLSAYII RVTTELNIVD EIIKSGGLSS GFYFEIARIE NEMKINRQIL DNAAKYVEHD
PRLVAEHRFE NMKPNFWSRI GTAATKRYPG VMYAFTTPLI SFFGLFDINV IGLIVILFIM
FMLIFNVKSK LLWFLTGTFV TAFI
