ID   H3_VAR67                Reviewed;         325 AA.
AC   P33059;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   02-JUN-2021, entry version 73.
DE   RecName: Full=Envelope protein H3;
DE   AltName: Full=Ag35;
DE   AltName: Full=Virion envelope protein p35;
GN   ORFNames=H3L, I3L;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8383392; DOI=10.1016/0168-1702(93)90110-9;
RA   Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S.,
RA   Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V.,
RA   Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G.,
RA   Sandakhchiev L.S.;
RT   "Nucleotide sequence analysis of variola virus HindIII M, L, I genome
RT   fragments.";
RL   Virus Res. 27:25-35(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Envelope protein that binds to heparan sulfate on the cell
CC       surface and might provide virion attachment to target cell.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Single-pass
CC       membrane protein {ECO:0000250}. Note=Component of the mature virion
CC       (MV) membrane. Becomes membrane associated presumably during virus
CC       maturation. The mature virion is located in the cytoplasm of infected
CC       cells and is probably released by cell lysis (By similarity).
CC       {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- PTM: Does not contain disulfide bonds. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Immunodominant protein. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae protein H3 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X67119; CAA47585.1; -; Genomic_DNA.
DR   EMBL; S55844; AAB24682.1; -; Genomic_DNA.
DR   EMBL; X69198; CAA49027.1; -; Genomic_DNA.
DR   PIR; S33100; S33100.
DR   RefSeq; NP_042130.1; NC_001611.1.
DR   SMR; P33059; -.
DR   PRIDE; P33059; -.
DR   GeneID; 1486441; -.
DR   KEGG; vg:1486441; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR004900; Poxvirus_P35.
DR   Pfam; PF03213; Pox_P35; 1.
PE   2: Evidence at transcript level;
KW   Host-virus interaction; Late protein; Membrane; Reference proteome;
KW   Signal-anchor; Transmembrane; Transmembrane helix;
KW   Viral attachment to host cell; Viral envelope protein; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..325
FT                   /note="Envelope protein H3"
FT                   /id="PRO_0000099209"
FT   TOPO_DOM        1..285
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical; Signal-anchor"
FT                   /evidence="ECO:0000305"
FT   TOPO_DOM        307..325
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   325 AA;  37696 MW;  EAB2FEE3CDEA6E40 CRC64;
     MATVNKTPVI VVPVIDRPPS ETFPNLHEHI NDQKFDDVKD NEVMPEKRNV VIVKDDPDHY
     KDYAFIHWTG GNIRNDDKYT HFFSGFCNTM CTEETKRNIA RHLALWDSKF FTELENKKVE
     YVVIVENDNV IEDITFLRPV LKAMHDKKID ILQMREIITG NKVKTELVMD KNHVIFTYTG
     GYDVSLSAYI IRVTTALNIV DEIIKSGGLS SGFYFEIARI ENEIKINRQI MDNSAKYVEH
     DPRLVAEHRF ENMKPNFWSR IGTAAVKRYP GVMYAFTTPL ISFFGLFDIN VIGLIVILFI
     MFMLIFNVKS KLLWFLTGTF VTAFI