ID   H4H3_MELV               Reviewed;         216 AA.
AC   A0A097I2D0;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   07-JAN-2015, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Histone doublet H4-H3 {ECO:0000303|PubMed:34297924};
DE   AltName: Full=Histone H4-H3 fusion protein {ECO:0000303|PubMed:34297924};
DE   AltName: Full=MV-H4-H3 {ECO:0000303|PubMed:34297924};
GN   ORFNames=MEL_368 {ECO:0000312|EMBL:AIT54981.1};
OS   Melbournevirus (MelV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC   Pimascovirales; Marseilleviridae; Marseillevirus;
OC   unclassified Marseillevirus.
OX   NCBI_TaxID=1560514;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=25275139; DOI=10.1128/jvi.02414-14;
RA   Doutre G., Philippe N., Abergel C., Claverie J.M.;
RT   "Genome analysis of the first Marseilleviridae representative from
RT   Australia indicates that most of its genes contribute to virus fitness.";
RL   J. Virol. 88:14340-14349(2014).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND DOMAIN.
RX   PubMed=34297924; DOI=10.1016/j.cell.2021.06.032;
RA   Liu Y., Bisio H., Toner C.M., Jeudy S., Philippe N., Zhou K., Bowerman S.,
RA   White A., Edwards G., Abergel C., Luger K.;
RT   "Virus-encoded histone doublets are essential and form nucleosome-like
RT   structures.";
RL   Cell 184:4237-4250.e19(2021).
CC   -!- FUNCTION: Histone-like protein that is recruited to viral factories
CC       during viral replication and participates in viral DNA packaging and
CC       virion production probably by forming unstable nucleosome-like
CC       particles (PubMed:34297924). May compact the viral DNA
CC       (PubMed:34297924). {ECO:0000269|PubMed:34297924}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:34297924}. Host
CC       cytoplasm {ECO:0000269|PubMed:34297924}. Virion
CC       {ECO:0000269|PubMed:34297924}. Note=Localize to cytoplasmic viral
CC       factories after virus infection (PubMed:34297924). Also present in the
CC       nucleus but at much lower concentration (PubMed:34297924). The viral
CC       histones that localize in the nucleus apparently do not interact with
CC       host genomic DNA and can leave the nucleus to associate with the viral
CC       factory (PubMed:34297924). {ECO:0000269|PubMed:34297924}.
CC   -!- INDUCTION: Expression of viral histones begins 2-4 hpi and continues
CC       along the infectious cycle. {ECO:0000269|PubMed:34297924}.
CC   -!- DOMAIN: The N-terminus is similar to eukaryotic H4, whereas the C-
CC       terminus is similar to eukaryotic H3. {ECO:0000269|PubMed:34297924}.
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DR   EMBL; KM275475; AIT54981.1; -; Genomic_DNA.
DR   RefSeq; YP_009094869.1; NC_025412.1.
DR   PDB; 7N8N; EM; 3.89 A; A/C=2-216.
DR   PDBsum; 7N8N; -.
DR   SMR; A0A097I2D0; -.
DR   GeneID; 21012389; -.
DR   KEGG; vg:21012389; -.
DR   Proteomes; UP000207668; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.20.10; -; 2.
DR   InterPro; IPR009072; Histone-fold.
DR   InterPro; IPR007125; Histone_H2A/H2B/H3.
DR   Pfam; PF00125; Histone; 1.
DR   SUPFAM; SSF47113; SSF47113; 2.
PE   1: Evidence at protein level;
KW   3D-structure; DNA condensation; Host cytoplasm; Host nucleus; Virion.
FT   CHAIN           1..216
FT                   /note="Histone doublet H4-H3"
FT                   /id="PRO_0000454838"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   216 AA;  23520 MW;  8C43120A009E5D21 CRC64;
     MSKAGKKVKA QQHGHLADHV SVGETQIPKA STQHLLRKAG SLSAAGDTEV PIRGFVHMKL
     HKLVQKSLLA MQLAKRKTIM KSDVKKAAEL MHLPVFAIPT KDSGAKGSVF LSCRQKGAGS
     AGTGSETNSQ EVRSQMKSTC LIIPKERFRT MAKEISKKEG HDVHIAEAAL DMLQVIVESC
     TVRLLEKALV ITYSGKRTRV TSKDIETAFM LEHGPL