ID   H5_VACCC                Reviewed;         203 AA.
AC   P20538;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   23-FEB-2022, entry version 69.
DE   RecName: Full=Late transcription elongation factor H5;
DE   AltName: Full=Viral late gene transcription factor 4;
DE            Short=VLTF-4;
GN   ORFNames=H5R;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Involved in the co-transcriptional or post-transcriptional
CC       endoribonucleolytic cleavage that generates sequence-homogeneous 3'
CC       ends during late transcription. Involved in postreplicative
CC       transcription elongation on intermediate and late genes (Probable).
CC       Also involved in DNA replication and in multiple steps of virion
CC       morphogenesis. Required both for inclusion of virosoplasm into
CC       crescents as well as for maturation of immature virions (IV) into
CC       mature virions (MV) (By similarity). {ECO:0000250, ECO:0000305}.
CC   -!- SUBUNIT: Interacts with the DNA polymerase processivity factor A20.
CC       Interacts with B1R kinase. Interacts with the late transcription
CC       factors VLTF-1 AND VLTF-3. Interacts with the late transcription
CC       elongation factor G2. Interacts with itself. Might be part of a
CC       transcription complex composed at least of G2, A18, and H5 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}. Note=Early during viral infection, diffusely localizes
CC       within the cytoplasm. Following DNA replication, localizes specifically
CC       to virus factories (By similarity). {ECO:0000250}.
CC   -!- INDUCTION: Constitutively expressed in abundance during viral
CC       replication.
CC   -!- PTM: Phosphorylated at multiple sites. Phosphorylation is necessary for
CC       cleavage activity. Phosphorylated by the viral B1R and F10 kinases (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae protein H5 family.
CC       {ECO:0000305}.
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DR   EMBL; M35027; AAA48092.1; -; Genomic_DNA.
DR   PIR; E42514; E42514.
DR   SMR; P20538; -.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019031; C:viral envelope; IEA:InterPro.
DR   GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004966; Pox_Ag35.
DR   Pfam; PF03286; Pox_Ag35; 1.
PE   2: Evidence at transcript level;
KW   Elongation factor; Host cytoplasm; Phosphoprotein; Protein biosynthesis;
KW   Reference proteome; Transcription; Transcription regulation; Virion.
FT   CHAIN           1..203
FT                   /note="Late transcription elongation factor H5"
FT                   /id="PRO_0000099203"
FT   REGION          25..74
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          80..99
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        25..39
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         84
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         85
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   203 AA;  22270 MW;  E2D8E0EB2DD67F2C CRC64;
     MAWSITNKAD TSSFTKMAEI RAHLKNSAEN KDKNEDIFPE DVIIPSTKPK TKRATTPRKP
     AATKRSTKKE EVEEEVVIEE YHQTTEKNSP SPGVGDIVES VAAVELDDSD GDDEPMVQVE
     AGKVNHSARS DLSDLKVATD NIVKDLKKII TRISAVSTVL EDVQAAGISR QFTSMTKAIT
     TLSDLVTEGK SKVVRKKVKT CKK