H5_VACCW
ID H5_VACCW Reviewed; 203 AA.
AC P07242; Q76ZS8;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 23-FEB-2022, entry version 80.
DE RecName: Full=Late transcription elongation factor H5;
DE AltName: Full=Viral late gene transcription factor 4;
DE Short=VLTF-4;
GN OrderedLocusNames=VACWR103; ORFNames=H5R;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3021979; DOI=10.1128/jvi.60.2.436-449.1986;
RA Rosel J.L., Earl P.L., Weir J.P., Moss B.;
RT "Conserved TAAATG sequence at the transcriptional and translational
RT initiation sites of vaccinia virus late genes deduced by structural and
RT functional analysis of the HindIII H genome fragment.";
RL J. Virol. 60:436-449(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2462305;
RA Gordon J., Kovala T., Dales S.;
RT "Molecular characterization of a prominent antigen of the vaccinia virus
RT envelope.";
RL Virology 167:361-369(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Dts57, IHDW, and WR;
RX PubMed=20206959; DOI=10.1016/j.virol.2010.01.020;
RA D'Costa S.M., Bainbridge T.W., Kato S.E., Prins C., Kelley K., Condit R.C.;
RT "Vaccinia H5 is a multifunctional protein involved in viral DNA
RT replication, postreplicative gene transcription, and virion
RT morphogenesis.";
RL Virology 401:49-60(2010).
RN [5]
RP IDENTIFICATION, AND SUBCELLULAR LOCATION.
RX PubMed=8794318; DOI=10.1128/jvi.70.10.6796-6802.1996;
RA Kovacs G.R., Moss B.;
RT "The vaccinia virus H5R gene encodes late gene transcription factor 4:
RT purification, cloning, and overexpression.";
RL J. Virol. 70:6796-6802(1996).
RN [6]
RP INTERACTION WITH THE LATE TRANSCRIPTION ELONGATION FACTOR G2, AND POSSIBLE
RP IDENTIFICATION IN A COMPLEX WITH A18 AND G2.
RX PubMed=9636370; DOI=10.1006/viro.1998.9166;
RA Black E.P., Moussatche N., Condit R.C.;
RT "Characterization of the interactions among vaccinia virus transcription
RT factors G2R, A18R, and H5R.";
RL Virology 245:313-322(1998).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=10769065; DOI=10.1099/0022-1317-81-5-1231;
RA Domi A., Beaud G.;
RT "The punctate sites of accumulation of vaccinia virus early proteins are
RT precursors of sites of viral DNA synthesis.";
RL J. Gen. Virol. 81:1231-1235(2000).
RN [8]
RP PHOSPHORYLATION AT THR-84 AND THR-85.
RX PubMed=11001589; DOI=10.1186/1471-2091-1-2;
RA Brown N.G., Nick Morrice D., Beaud G., Hardie G., Leader D.P.;
RT "Identification of sites phosphorylated by the vaccinia virus B1R kinase in
RT viral protein H5R.";
RL BMC Biochem. 1:2-2(2000).
RN [9]
RP INTERACTION WITH A20.
RX PubMed=12490386; DOI=10.1006/viro.2002.1721;
RA Ishii K., Moss B.;
RT "Mapping interaction sites of the A20R protein component of the vaccinia
RT virus DNA replication complex.";
RL Virology 303:232-239(2002).
RN [10]
RP INTERACTION WITH VLTF-1 AND VLTF-3.
RX PubMed=15518812; DOI=10.1016/j.virol.2004.08.017;
RA Dellis S., Strickland K.C., McCrary W.J., Patel A., Stocum E., Wright C.F.;
RT "Protein interactions among the vaccinia virus late transcription
RT factors.";
RL Virology 329:328-336(2004).
RN [11]
RP FUNCTION.
RX PubMed=17376501; DOI=10.1016/j.virol.2007.02.016;
RA Cresawn S.G., Condit R.C.;
RT "A targeted approach to identification of vaccinia virus postreplicative
RT transcription elongation factors: genetic evidence for a role of the H5R
RT gene in vaccinia transcription.";
RL Virology 363:333-341(2007).
RN [12]
RP FUNCTION, AND PHOSPHORYLATION.
RX PubMed=18089571; DOI=10.1074/jbc.m709258200;
RA D'Costa S.M., Bainbridge T.W., Condit R.C.;
RT "Purification and properties of the vaccinia virus mRNA processing
RT factor.";
RL J. Biol. Chem. 283:5267-5275(2008).
CC -!- FUNCTION: Involved in the co-transcriptional or post-transcriptional
CC endoribonucleolytic cleavage that generates sequence-homogeneous 3'
CC ends during late transcription. Involved in postreplicative
CC transcription elongation on intermediate and late genes (Probable).
CC Also involved in DNA replication and in multiple steps of virion
CC morphogenesis. Required both for inclusion of virosoplasm into
CC crescents as well as for maturation of immature virions (IV) into
CC mature virions (MV). {ECO:0000269|PubMed:17376501,
CC ECO:0000269|PubMed:18089571, ECO:0000269|PubMed:20206959, ECO:0000305}.
CC -!- SUBUNIT: Interacts with the DNA polymerase processivity factor A20.
CC Interacts with B1R kinase. Interacts with the late transcription
CC factors VLTF-1 AND VLTF-3. Interacts with the late transcription
CC elongation factor G2. Interacts with itself. Might be part of a
CC transcription complex composed at least of G2, A18, and H5.
CC {ECO:0000269|PubMed:12490386, ECO:0000269|PubMed:15518812,
CC ECO:0000269|PubMed:9636370}.
CC -!- INTERACTION:
CC P07242; P68456: VACWR080; NbExp=4; IntAct=EBI-7272435, EBI-7273347;
CC P07242; P68613: VLTF1; NbExp=4; IntAct=EBI-7272435, EBI-7273218;
CC P07242; P68318: VLTF3; Xeno; NbExp=2; IntAct=EBI-7272435, EBI-7366338;
CC -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm. Note=Early during viral
CC infection, diffusely localizes within the cytoplasm. Following DNA
CC replication, localizes specifically to virus factories.
CC -!- INDUCTION: Constitutively expressed in abundance during viral
CC replication.
CC -!- PTM: Phosphorylated at multiple sites. Phosphorylation is necessary for
CC cleavage activity. Phosphorylated by the viral B1R and F10 kinases
CC (Probable). {ECO:0000305|PubMed:11001589, ECO:0000305|PubMed:18089571}.
CC -!- SIMILARITY: Belongs to the chordopoxvirinae protein H5 family.
CC {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be p35/Ag35, a membrane protein
CC involved in the biogenesis of the viral envelope encoded by H3L gene.
CC {ECO:0000305|PubMed:2462305}.
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DR EMBL; M13209; AAB59841.1; -; Genomic_DNA.
DR EMBL; M23648; AAA47962.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89382.1; -; Genomic_DNA.
DR PIR; F24481; QQVZH6.
DR RefSeq; YP_232985.1; NC_006998.1.
DR SMR; P07242; -.
DR DIP; DIP-2164N; -.
DR IntAct; P07242; 9.
DR MINT; P07242; -.
DR iPTMnet; P07242; -.
DR DNASU; 3707559; -.
DR GeneID; 3707559; -.
DR KEGG; vg:3707559; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IDA:UniProtKB.
DR GO; GO:0019031; C:viral envelope; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0039693; P:viral DNA genome replication; IDA:UniProtKB.
DR GO; GO:0019083; P:viral transcription; IDA:UniProtKB.
DR InterPro; IPR004966; Pox_Ag35.
DR Pfam; PF03286; Pox_Ag35; 1.
PE 1: Evidence at protein level;
KW Elongation factor; Host cytoplasm; Phosphoprotein; Protein biosynthesis;
KW Reference proteome; Transcription; Transcription regulation; Virion.
FT CHAIN 1..203
FT /note="Late transcription elongation factor H5"
FT /id="PRO_0000099204"
FT REGION 25..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..39
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 63..82
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 84
FT /note="Phosphothreonine"
FT /evidence="ECO:0000305|PubMed:11001589"
FT MOD_RES 85
FT /note="Phosphothreonine"
FT /evidence="ECO:0000305|PubMed:11001589"
FT VARIANT 189
FT /note="G -> R (in strain: Dts57)"
SQ SEQUENCE 203 AA; 22300 MW; 89B54D5DFD976B7D CRC64;
MAWSITNKAD TSSFTKMAEI RAHLKNSAEN KDKNEDIFPE DVIIPSTKPK TKRATTPRKP
AATKRSTKKE EVEEEVVIEE YHQTTEKNSP SPGVSDIVES VAAVELDDSD GDDEPMVQVE
AGKVNHSARS DLSDLKVATD NIVKDLKKII TRISAVSTVL EDVQAAGISR QFTSMTKAIT
TLSDLVTEGK SKVVRKKVKT CKK
