ID   H5_VAR67                Reviewed;         221 AA.
AC   P33062;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   23-FEB-2022, entry version 71.
DE   RecName: Full=Late transcription elongation factor H5;
DE   AltName: Full=Viral late gene transcription factor 4;
DE            Short=VLTF-4;
GN   ORFNames=H5R;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8383392; DOI=10.1016/0168-1702(93)90110-9;
RA   Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S.,
RA   Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V.,
RA   Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G.,
RA   Sandakhchiev L.S.;
RT   "Nucleotide sequence analysis of variola virus HindIII M, L, I genome
RT   fragments.";
RL   Virus Res. 27:25-35(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Involved in the co-transcriptional or post-transcriptional
CC       endoribonucleolytic cleavage that generates sequence-homogeneous 3'
CC       ends during late transcription. Involved in postreplicative
CC       transcription elongation on intermediate and late genes (Probable).
CC       Also involved in DNA replication and in multiple steps of virion
CC       morphogenesis. Required both for inclusion of virosoplasm into
CC       crescents as well as for maturation of immature virions (IV) into
CC       mature virions (MV) (By similarity). {ECO:0000250, ECO:0000305}.
CC   -!- SUBUNIT: Interacts with the DNA polymerase processivity factor A20.
CC       Interacts with B1R kinase. Interacts with the late transcription
CC       factors VLTF-1 AND VLTF-3. Interacts with the late transcription
CC       elongation factor G2. Interacts with itself. Might be part of a
CC       transcription complex composed at least of G2, A18, and H5 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}. Note=Early during viral infection, diffusely localizes
CC       within the cytoplasm. Following DNA replication, localizes specifically
CC       to virus factories (By similarity). {ECO:0000250}.
CC   -!- INDUCTION: Constitutively expressed in abundance during viral
CC       replication.
CC   -!- PTM: Phosphorylated at multiple sites. Phosphorylation is necessary for
CC       cleavage activity. Phosphorylated by the viral B1R and F10 kinases (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae protein H5 family.
CC       {ECO:0000305}.
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DR   EMBL; X67119; CAA47587.1; -; Genomic_DNA.
DR   EMBL; S55844; AAB24684.1; -; Genomic_DNA.
DR   EMBL; X69198; CAA49029.1; -; Genomic_DNA.
DR   PIR; S33102; S33102.
DR   RefSeq; NP_042132.1; NC_001611.1.
DR   SMR; P33062; -.
DR   PRIDE; P33062; -.
DR   GeneID; 1486443; -.
DR   KEGG; vg:1486443; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019031; C:viral envelope; IEA:InterPro.
DR   GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004966; Pox_Ag35.
DR   Pfam; PF03286; Pox_Ag35; 1.
PE   2: Evidence at transcript level;
KW   Elongation factor; Host cytoplasm; Phosphoprotein; Protein biosynthesis;
KW   Reference proteome; Transcription; Transcription regulation; Virion.
FT   CHAIN           1..221
FT                   /note="Late transcription elongation factor H5"
FT                   /id="PRO_0000099205"
FT   REGION          25..105
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        25..39
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        88..105
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         89
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         90
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   221 AA;  24359 MW;  EE2AF40068A15B8C CRC64;
     MAWSITNKAD TSSFTKMAEI RAHLRNSAEN KDKNDDIFPE DVIIPSTKPK TKRATTPRKP
     AATKRSTKKD KEKEEVEEEE VVIEEYHQTT EENSPPPSSS PGVGNIVESV TAVELDDSNG
     DDDNDNDNDD NEPMVQVEAG KVNHSARSDL SDLKVATDNI VKDLKKIITR ISAVSTVLED
     VQAAGISRQF TSMTKSITTL SDLVTEGKSK VVRKKVKTCK K