H669_METJA
ID H669_METJA Reviewed; 367 AA.
AC Q58083;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Probable ATP-dependent RNA helicase MJ0669;
DE EC=3.6.4.13;
GN OrderedLocusNames=MJ0669;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RX PubMed=11171974; DOI=10.1073/pnas.98.4.1465;
RA Story R.M., Li H., Abelson J.N.;
RT "Crystal structure of a DEAD box protein from the hyperthermophile
RT Methanococcus jannaschii.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:1465-1470(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. {ECO:0000305}.
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DR EMBL; L77117; AAB98663.1; -; Genomic_DNA.
DR PIR; E64383; E64383.
DR PDB; 1HV8; X-ray; 3.00 A; A/B=1-367.
DR PDBsum; 1HV8; -.
DR AlphaFoldDB; Q58083; -.
DR SMR; Q58083; -.
DR STRING; 243232.MJ_0669; -.
DR REBASE; 203805; Ppe892ORF686P.
DR REBASE; 205577; Ppe194ORF513P.
DR PRIDE; Q58083; -.
DR EnsemblBacteria; AAB98663; AAB98663; MJ_0669.
DR KEGG; mja:MJ_0669; -.
DR eggNOG; arCOG00558; Archaea.
DR HOGENOM; CLU_003041_1_3_2; -.
DR InParanoid; Q58083; -.
DR OMA; EHKDGQR; -.
DR PhylomeDB; Q58083; -.
DR EvolutionaryTrace; Q58083; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Helicase; Hydrolase; Nucleotide-binding;
KW Reference proteome; RNA-binding.
FT CHAIN 1..367
FT /note="Probable ATP-dependent RNA helicase MJ0669"
FT /id="PRO_0000055116"
FT DOMAIN 38..206
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 213..367
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 6..34
FT /note="Q motif"
FT MOTIF 154..157
FT /note="DEAD box"
FT BINDING 51..58
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT HELIX 8..10
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 15..24
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 31..42
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 45..50
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 53..56
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 57..68
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 71..74
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 77..80
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 84..98
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 105..108
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 114..122
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 125..129
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 131..139
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 150..155
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 156..160
FT /evidence="ECO:0007829|PDB:1HV8"
FT TURN 161..164
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 165..173
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 180..184
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 190..199
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 202..207
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 210..220
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 223..225
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 226..234
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 241..244
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 248..260
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 265..268
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 270..272
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 274..285
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 288..294
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 298..301
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 308..314
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 319..325
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 332..334
FT /evidence="ECO:0007829|PDB:1HV8"
FT STRAND 337..342
FT /evidence="ECO:0007829|PDB:1HV8"
FT HELIX 347..357
FT /evidence="ECO:0007829|PDB:1HV8"
SQ SEQUENCE 367 AA; 41920 MW; 1B9102E8DF50CB37 CRC64;
MEVEYMNFNE LNLSDNILNA IRNKGFEKPT DIQMKVIPLF LNDEYNIVAQ ARTGSGKTAS
FAIPLIELVN ENNGIEAIIL TPTRELAIQV ADEIESLKGN KNLKIAKIYG GKAIYPQIKA
LKNANIVVGT PGRILDHINR GTLNLKNVKY FILDEADEML NMGFIKDVEK ILNACNKDKR
ILLFSATMPR EILNLAKKYM GDYSFIKAKI NANIEQSYVE VNENERFEAL CRLLKNKEFY
GLVFCKTKRD TKELASMLRD IGFKAGAIHG DLSQSQREKV IRLFKQKKIR ILIATDVMSR
GIDVNDLNCV INYHLPQNPE SYMHRIGRTG RAGKKGKAIS IINRREYKKL RYIERAMKLK
IKKLKFG
