ID   H7_VACCW                Reviewed;         146 AA.
AC   P08586; Q80HW1;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 2.
DT   23-FEB-2022, entry version 63.
DE   RecName: Full=Late protein H7;
DE   AltName: Full=Protein H8;
GN   OrderedLocusNames=VACWR105; ORFNames=H7R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-129.
RX   PubMed=3021979; DOI=10.1128/jvi.60.2.436-449.1986;
RA   Rosel J.L., Earl P.L., Weir J.P., Moss B.;
RT   "Conserved TAAATG sequence at the transcriptional and translational
RT   initiation sites of vaccinia virus late genes deduced by structural and
RT   functional analysis of the HindIII H genome fragment.";
RL   J. Virol. 60:436-449(1986).
RN   [3]
RP   FUNCTION, INDUCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=19553304; DOI=10.1128/jvi.00877-09;
RA   Satheshkumar P.S., Weisberg A., Moss B.;
RT   "Vaccinia virus H7 protein contributes to the formation of crescent
RT   membrane precursors of immature virions.";
RL   J. Virol. 83:8439-8450(2009).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS), AND FUNCTION.
RX   PubMed=25473060; DOI=10.1128/jvi.03073-14;
RA   Kolli S., Meng X., Wu X., Shengjuler D., Cameron C.E., Xiang Y., Deng J.;
RT   "Structure-function analysis of vaccinia virus H7 protein reveals a novel
RT   phosphoinositide binding fold essential for poxvirus replication.";
RL   J. Virol. 89:2209-2219(2015).
CC   -!- FUNCTION: Contributes to the formation of crescents and immature
CC       virions (IV). Interacts with phosphatidylinositol-3-phosphate (PI3P)
CC       and phosphatidylinositol-4-phosphate (PI4P) lipids in order to form
CC       virion membranes. {ECO:0000269|PubMed:19553304,
CC       ECO:0000269|PubMed:25473060}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}. Host cytoplasm {ECO:0000269|PubMed:19553304}.
CC       Note=Probably transitorily part of the membrane of crescents during
CC       immature virions formation. Not incorporated into virions. Probably
CC       synthesized, but not retained in viral factories.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:19553304}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae H7 family. {ECO:0000305}.
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DR   EMBL; AY243312; AAO89384.1; -; Genomic_DNA.
DR   EMBL; M13209; AAB59843.1; -; Genomic_DNA.
DR   PIR; H24481; QQVZH8.
DR   RefSeq; YP_232987.1; NC_006998.1.
DR   PDB; 4W5X; X-ray; 2.00 A; A/B=1-118.
DR   PDB; 4W60; X-ray; 2.70 A; A/B/C/D=1-146.
DR   PDBsum; 4W5X; -.
DR   PDBsum; 4W60; -.
DR   SMR; P08586; -.
DR   DNASU; 3707561; -.
DR   GeneID; 3707561; -.
DR   KEGG; vg:3707561; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR006872; Poxvirus_H7.
DR   Pfam; PF04787; Pox_H7; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Host cytoplasm; Late protein; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..146
FT                   /note="Late protein H7"
FT                   /id="PRO_0000099553"
FT   TRANSMEM        10..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        29
FT                   /note="M -> V (in Ref. 2; AAB59843)"
FT                   /evidence="ECO:0000305"
FT   HELIX           5..12
FT                   /evidence="ECO:0007829|PDB:4W5X"
FT   TURN            13..18
FT                   /evidence="ECO:0007829|PDB:4W5X"
FT   HELIX           21..32
FT                   /evidence="ECO:0007829|PDB:4W5X"
FT   STRAND          37..43
FT                   /evidence="ECO:0007829|PDB:4W5X"
FT   STRAND          49..54
FT                   /evidence="ECO:0007829|PDB:4W5X"
FT   HELIX           60..64
FT                   /evidence="ECO:0007829|PDB:4W5X"
FT   HELIX           75..78
FT                   /evidence="ECO:0007829|PDB:4W5X"
FT   HELIX           80..95
FT                   /evidence="ECO:0007829|PDB:4W5X"
FT   HELIX           97..102
FT                   /evidence="ECO:0007829|PDB:4W5X"
FT   HELIX           105..114
FT                   /evidence="ECO:0007829|PDB:4W5X"
SQ   SEQUENCE   146 AA;  16932 MW;  1B21D8D7989566A8 CRC64;
     MEMDKRMKSL AMTAFFGELS TLDIMALIMS IFKRHPNNTI FSVDKDGQFM IDFEYDNYKA
     SQYLDLTLTP ISGDECKTHA SSIAEQLACV DIIKEDISEY IKTTPRLKRF IKKYRNRSDT
     RISRDTEKLK IALAKGIDYE YIKDAC