H962L_ASFB7
ID H962L_ASFB7 Reviewed; 962 AA.
AC Q89443;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Putative RNA helicase B962L;
DE EC=3.6.4.13;
GN OrderedLocusNames=Ba71V-072; ORFNames=B962L;
OS African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS (ASFV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Asfuvirales; Asfarviridae; Asfivirus.
OX NCBI_TaxID=10498;
OH NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8262374; DOI=10.1016/0378-1119(93)90090-p;
RA Yanez R.J., Rodriguez J.M., Boursnell M.E., Rodriguez J.F., Vinuela E.;
RT "Two putative African swine fever virus helicases similar to yeast 'DEAH'
RT pre-mRNA processing proteins and vaccinia virus ATPases D11L and D6R.";
RL Gene 134:161-174(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA Rodriguez J.F., Vinuela E.;
RT "Analysis of the complete nucleotide sequence of African swine fever
RT virus.";
RL Virology 208:249-278(1995).
RN [3]
RP INDUCTION.
RX PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA Dixon L., Werner F.;
RT "The African Swine Fever Virus Transcriptome.";
RL J. Virol. 94:0-0(2020).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=30185597; DOI=10.1128/jvi.01293-18;
RA Alejo A., Matamoros T., Guerra M., Andres G.;
RT "A Proteomic Atlas of the African Swine Fever Virus Particle.";
RL J. Virol. 92:0-0(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}. Virion {ECO:0000269|PubMed:30185597}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC {ECO:0000269|PubMed:32075923}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L10062; AAA42698.1; -; Genomic_DNA.
DR EMBL; U18466; AAA65302.1; -; Genomic_DNA.
DR PIR; JT0669; JT0669.
DR RefSeq; NP_042766.1; NC_001659.2.
DR SMR; Q89443; -.
DR GeneID; 22220302; -.
DR KEGG; vg:22220302; -.
DR Proteomes; UP000000624; Genome.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Host membrane; Hydrolase; Late protein; Membrane;
KW Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix;
KW Virion.
FT CHAIN 1..962
FT /note="Putative RNA helicase B962L"
FT /id="PRO_0000373102"
FT TRANSMEM 521..541
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 43..229
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 253..459
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 167..170
FT /note="DEAH box"
FT BINDING 56..63
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 962 AA; 109630 MW; EDA8F0CD02F3D2AC CRC64;
MGKPTLLEPG HLYNVPAEHK NDVPIHYIIT WIKQRLPEFG GAIPTSLADR VLIIKSRTGS
GKSTALPVHV FRILRNENTH SFQKYLGRSV ICTQPRVLTA VTLAKDIGAS THYPDMILGQ
TVGYHTKPLT EKPNRGLIYA TAGVLLAQLH TMTDDEIASR YAFMIIDEAH ERALGIDLML
MYIKSMLQRM LQRGSIGALR IPFVILTSAT IDTHKYSTYF GIGKENIILV EGRQYGVETH
WPLYNTNNYI KTACETALTI HKENIHDRPT EADILIFMPG MAEIRFLSML LNNANMDLAK
EKLPLMLILP IDSEAIAQEN EAYLGLKAEI KNLWVKNPLT AKVEKPLRRV IVSTVVAETG
LTIETLKYVI DPGWNRSIET YYPEWAGGLI TRPAAQSRIE QRKGRVGRVF PGHFYPLYTK
HVFEQIPAQQ YPEIITEGPG AIFLSIVVET IKKNKEGVFK AEEIDMLDPP PTDALASAIE
RAIVAGLLTR GEKGLQLTQL GDIASRFSFL SIEEARMCFS GYFWQAAISD IATILAVVSV
ADKKLTNLLD SKQRNGAMLA EAVLAGIPPF LQNIDNAYTN IHLLLADDLL EGLFIFEGFQ
HAIVYFINNK VNNVAKHLRE WCEKKMLKYS SMVQILARRE DILNELAIVG LNPFHQWQNR
LASANAETFL KRVCTLKQCM YEAYRLNCFC YDKHRLLYTG RNGIHFSYHD AVIKNPSCIV
TPRIMLSPVS KQYMEWRLEP SFVSVLDGFV NVDINFLSPR QEIPNILGGV EDEEEEPPLP
IQVFLHKYVK THFHFSGKSF KELKMKPSQM IKFPETTLIN MIPDIPKNVV QTYLEINVCH
QYSFKRLIYC ETFYTDMDDV QHENSVELIG LPMAAHHLTI NDFNKLYHLL KPDGFLIVYD
LHKGQEAFWL HSLQDALGHH TIRRDMDFHT IPEWETIFKE CGFTPIFSKQ PSEHELFIVF
KK
