ID   HA17C_CBCP              Reviewed;         146 AA.
AC   P46083;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   29-SEP-2021, entry version 76.
DE   RecName: Full=Hemagglutinin component HA-17 type C {ECO:0000303|PubMed:7802661};
DE   AltName: Full=ANTP17;
DE   AltName: Full=HA 17 kDa subunit;
DE   AltName: Full=HA2;
GN   Name=HA-17;
GN   Synonyms=antP-17 {ECO:0000303|PubMed:8028579},
GN   cha-17 {ECO:0000303|PubMed:7802661};
OS   Clostridium botulinum C phage (Clostridium botulinum C bacteriophage).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae.
OX   NCBI_TaxID=12336;
OH   NCBI_TaxID=36828; Clostridium botulinum C.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21, SUBUNIT, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=Stockholm / Type C / phage C-ST;
RX   PubMed=7802661; DOI=10.1006/bbrc.1994.2805;
RA   Fujinaga Y., Inoue K., Shimazaki S., Tomochika K., Tsuzuki K., Fujii N.,
RA   Watanabe T., Ohyama T., Takeshi K., Inoue K., Oguma K.;
RT   "Molecular construction of Clostridium botulinum type C progenitor toxin
RT   and its gene organization.";
RL   Biochem. Biophys. Res. Commun. 205:1291-1298(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=C-468 / Type C / phage 1C;
RX   PubMed=8028579; DOI=10.1007/bf00279572;
RA   Hauser D.F., Eklund M.W., Boquet P., Popoff M.R.;
RT   "Organization of the botulinum neurotoxin C1 gene and its associated non-
RT   toxic protein genes in Clostridium botulinum C 468.";
RL   Mol. Gen. Genet. 243:631-640(1994).
RN   [3]
RP   FUNCTION IN TOXICITY.
RC   STRAIN=Stockholm / Type C;
RX   PubMed=9421908; DOI=10.1099/00221287-143-12-3841;
RA   Fujinaga Y., Inoue K., Watanabe S., Yokota K., Hirai Y., Nagamachi E.,
RA   Oguma K.;
RT   "The haemagglutinin of Clostridium botulinum type C progenitor toxin plays
RT   an essential role in binding of toxin to the epithelial cells of guinea pig
RT   small intestine, leading to the efficient absorption of the toxin.";
RL   Microbiology 143:3841-3847(1997).
RN   [4]
RP   PRELIMINARY CRYSTALLIZATION.
RC   STRAIN=Type C;
RX   PubMed=24419620; DOI=10.1107/s2053230x13032378;
RA   Iwasa C., Tonozuka T., Shinoda M., Sagane Y., Niwa K., Watanabe T.,
RA   Yoshida H., Kamitori S., Takao T., Oguma K., Nishikawa A.;
RT   "Purification, crystallization and preliminary X-ray analysis of an HA17-
RT   HA70 (HA2-HA3) complex from Clostridium botulinum type C progenitor
RT   toxin.";
RL   Acta Crystallogr. F Struct. Biol. Commun. 70:64-67(2014).
CC   -!- FUNCTION: The hemagglutinin (HA) component of the progenitor toxin
CC       protects the structural integrity of botulinum neurotoxin; may increase
CC       internalization of the neurotoxin into the bloodstream of the host
CC       (PubMed:9421908). The HA component is involved in binding to the upper
CC       small intestine through interactions with glycolipids and glycoproteins
CC       containing sialic acid moieties (Probable).
CC       {ECO:0000269|PubMed:9421908, ECO:0000305|PubMed:9421908}.
CC   -!- SUBUNIT: Botulinum toxins are produced as progenitor toxins of large
CC       molecular sizes of 12S (M toxin) and 16S (L toxin). M toxin consists of
CC       a non-toxic, non-hemagglutinin component (NTNHA) and the neurotoxin
CC       (Probable). L toxin consists of the M toxin and the 3 subcomponents of
CC       hemagglutinin (HA) (PubMed:7802661). HA is composed of subcomponents of
CC       70, 33, and 17 kDa (PubMed:7802661). The 70 kDa subcomponent undergoes
CC       proteolytic processing and is split into HA-55 (also called HA-53 and
CC       HA3b) and HA-22-23 (also called HA3a) (PubMed:7802661). Preliminary
CC       crystallization studies show a tri-lobed HA-70 where each HA-70
CC       contacts one HA-17 molecule (Probable). The stoichiometry of the whole
CC       complex has been modeled as one BoNT/C, one NTNHA, three HA-70, six HA-
CC       33 and three HA-17 (By similarity). {ECO:0000250|UniProtKB:Q9LBR4,
CC       ECO:0000269|PubMed:7802661, ECO:0000305|PubMed:24419620,
CC       ECO:0000305|PubMed:7802661}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:7802661}.
CC   -!- MISCELLANEOUS: This protein can also be encoded on a prophage.
CC       {ECO:0000305}.
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DR   EMBL; X62389; CAA44260.1; -; Genomic_DNA.
DR   EMBL; S74768; AAB32848.1; -; Genomic_DNA.
DR   EMBL; X72793; CAA51310.1; -; Genomic_DNA.
DR   RefSeq; YP_398513.1; NC_007581.1.
DR   SMR; P46083; -.
DR   GeneID; 3772938; -.
DR   KEGG; vg:3772938; -.
DR   EvolutionaryTrace; P46083; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008903; HA-17_C_botulinum.
DR   InterPro; IPR035992; Ricin_B-like_lectins.
DR   PIRSF; PIRSF037660; Botulinum_HA-17; 1.
DR   SUPFAM; SSF50370; SSF50370; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hemagglutinin; Secreted; Virulence.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:7802661"
FT   CHAIN           2..146
FT                   /note="Hemagglutinin component HA-17 type C"
FT                   /id="PRO_0000083885"
SQ   SEQUENCE   146 AA;  16663 MW;  2145CE84C206D76E CRC64;
     MSSERTFLPN GNYKIKSLFS DSLYLTYSSG ALSFSNTSSL DNQKWKLEYI SSSNGFRFSN
     VAEPNKYLAY NDYGFIYLSS SSNNSLWNPI KIAINSYIIC TLSIVNVTDY AWTIYDNNNN
     ITDQPILNLP NFDINNSNQI LKLEKL