ID   HAAF_LIMPO              Reviewed;         172 AA.
AC   Q01528;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Hemagglutinin/amebocyte aggregation factor;
DE   AltName: Full=18K-LAF;
DE   Flags: Precursor;
OS   Limulus polyphemus (Atlantic horseshoe crab).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Merostomata;
OC   Xiphosura; Limulidae; Limulus.
OX   NCBI_TaxID=6850;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 20-82.
RX   PubMed=1429596; DOI=10.1016/s0021-9258(18)41693-6;
RA   Fujii N., Minetti C.A.S.A., Nakhasi H.L., Chen S.W., Barbehenn E.,
RA   Nunes P.H., Nguyen N.;
RT   "Isolation, cDNA cloning, and characterization of an 18-kDa hemagglutinin
RT   and amebocyte aggregation factor from Limulus polyphemus.";
RL   J. Biol. Chem. 267:22452-22459(1992).
CC   -!- FUNCTION: Possesses the property of inducing both aggregation of
CC       amebocytes and agglutination of erythrocytes.
CC   -!- SUBCELLULAR LOCATION: Secreted. Note=Secreted from amebocyte large
CC       secretory granules.
CC   -!- SIMILARITY: Belongs to the dermatopontin family. {ECO:0000305}.
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DR   EMBL; M96983; AAA28272.1; -; mRNA.
DR   PIR; A44312; A44312.
DR   RefSeq; XP_013791404.1; XM_013935950.1.
DR   AlphaFoldDB; Q01528; -.
DR   GeneID; 106475248; -.
DR   OrthoDB; 1271391at2759; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR026645; Dermatopontin_fam.
DR   PANTHER; PTHR15040; PTHR15040; 1.
DR   Pfam; PF14704; DERM; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Repeat; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:1429596"
FT   CHAIN           20..172
FT                   /note="Hemagglutinin/amebocyte aggregation factor"
FT                   /id="PRO_0000007370"
FT   REPEAT          21..25
FT                   /note="1-1"
FT   REPEAT          50..54
FT                   /note="2-1"
FT   REPEAT          73..77
FT                   /note="1-2"
FT   REPEAT          102..106
FT                   /note="2-2"
FT   REPEAT          129..133
FT                   /note="1-3"
FT   REPEAT          158..162
FT                   /note="2-3"
FT   DISULFID        32..58
FT                   /evidence="ECO:0000250"
FT   DISULFID        67..172
FT                   /evidence="ECO:0000250"
FT   DISULFID        84..110
FT                   /evidence="ECO:0000250"
FT   DISULFID        111..117
FT                   /evidence="ECO:0000250"
FT   DISULFID        123..167
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   172 AA;  20559 MW;  0434E4DCF870B9B5 CRC64;
     MNSPAIVIII FSTLTFSEAW VNDWDGALNF QCQLKDSIKT ISSIHSNHHE DRRWNFGCER
     TLRDPSCYFT NYVNDWDKLL HFTCKSGEAI AGFNSYHDNR REDRRWKIYC CKDKNKCTDY
     RTCAWTGYVN SWDGDLHYTV PKDYVLTGVI SEHDNHREDR RWKFQHCRLK NC