ID   HACT1_HELAT             Reviewed;          69 AA.
AC   P0DUS1;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   23-FEB-2022, entry version 2.
DE   RecName: Full=Peptide Hact-1 {ECO:0000303|PubMed:33166137};
DE   Flags: Precursor;
OS   Heliofungia actiniformis (Mushroom coral).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Scleractinia;
OC   Fungiina; Fungiidae; Heliofungia.
OX   NCBI_TaxID=75303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 58-69, MASS
RP   SPECTROMETRY, DISULFIDE BOND, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP   SYNTHESIS OF 58-69, AND STRUCTURE BY NMR.
RC   TISSUE=Tentacle;
RX   PubMed=33166137; DOI=10.1021/acs.jnatprod.0c00981;
RA   Schmidt C.A., Wilson D.T., Cooke I., Potriquet J., Tungatt K.,
RA   Muruganandah V., Boote C., Kuek F., Miles J.J., Kupz A., Ryan S.,
RA   Loukas A., Bansal P.S., Takjoo R., Miller D.J., Peigneur S., Tytgat J.,
RA   Daly N.L.;
RT   "Identification and characterization of a peptide from the stony coral
RT   Heliofungia actiniformis.";
RL   J. Nat. Prod. 83:3454-3463(2020).
CC   -!- FUNCTION: Peptide with unknown function. Has a limited effect on human
CC       peripheral blood mononuclear cells. Does not show activity against both
CC       Gram-positive and Gram-negative bacteria nor is it active on the 26
CC       voltage-gated ion channels tested. {ECO:0000269|PubMed:33166137}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33166137}.
CC       Nematocyst {ECO:0000305|PubMed:33166137}.
CC   -!- TISSUE SPECIFICITY: Tentacle (ecto and/or endoderm tissue), and
CC       possibly also nematoblasts. {ECO:0000269|PubMed:33166137}.
CC   -!- MASS SPECTROMETRY: Mass=1354.65; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:33166137};
CC   -!- MISCELLANEOUS: Shows a propensity to bind albumin in aqueous solution,
CC       which opens up the possibility of using it as a carrier for directing
CC       albumin binding as well as potentially increasing half-life in serum.
CC       {ECO:0000305|PubMed:33166137}.
CC   -!- MISCELLANEOUS: The peptide is found in two different conformations
CC       which could be explained by a cis-trans isomerization at Pro-63.
CC       {ECO:0000269|PubMed:33166137}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Nematocyst; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..57
FT                   /evidence="ECO:0000305|PubMed:33166137"
FT                   /id="PRO_0000453391"
FT   PEPTIDE         58..69
FT                   /note="Peptide Hact-1"
FT                   /evidence="ECO:0000269|PubMed:33166137"
FT                   /id="PRO_0000453392"
FT   DISULFID        59..68
FT                   /evidence="ECO:0000269|PubMed:33166137"
SQ   SEQUENCE   69 AA;  7925 MW;  5206C1C1E34F6020 CRC64;
     MDRKFHLCLL LVILGTIIVQ GAPLENENDA DPDKPQKYRY YLKRATTEKK DNDPAKPGCH
     YTPFGLICF