ID   HADD_PSEPU              Reviewed;         301 AA.
AC   Q52086;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 2.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=(R)-2-haloacid dehalogenase;
DE            EC=3.8.1.9;
DE   AltName: Full=D-2-haloacid dehalogenase;
DE   AltName: Full=D-DEX;
GN   Name=hadD;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AJ1;
RX   PubMed=1556080; DOI=10.1128/jb.174.8.2612-2619.1992;
RA   Barth P.T., Bolton L., Thomson J.C.;
RT   "Cloning and partial sequencing of an operon encoding two Pseudomonas
RT   putida haloalkanoate dehalogenases of opposite stereospecificity.";
RL   J. Bacteriol. 174:2612-2619(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10198020; DOI=10.1128/jb.181.8.2535-2547.1999;
RA   Hill K.E., Marchesi J.R., Weightman A.J.;
RT   "Investigation of two evolutionarily unrelated halocarboxylic acid
RT   dehalogenase gene families.";
RL   J. Bacteriol. 181:2535-2547(1999).
CC   -!- FUNCTION: Catalyzes the hydrolytic dehalogenation of small (R)-2-
CC       haloalkanoic acids to yield the corresponding (S)-2-hydroxyalkanoic
CC       acids. Acts on acids of short chain lengths, C(2) to C(4), with
CC       inversion of configuration at C-2.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an (R)-2-haloacid + H2O = a (2S)-2-hydroxycarboxylate + a
CC         halide anion + H(+); Xref=Rhea:RHEA:22188, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:58123,
CC         ChEBI:CHEBI:137406; EC=3.8.1.9;
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. S-2-
CC       haloalkanoic acid dehalogenase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA25831.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M81841; AAA25831.1; ALT_FRAME; Genomic_DNA.
DR   PIR; B41840; B41840.
DR   PDB; 5GZX; X-ray; 2.35 A; A/B/C/D=1-301.
DR   PDB; 5GZY; X-ray; 2.18 A; A/B/C/D=1-301.
DR   PDB; 5H00; X-ray; 2.64 A; A/B/C/D=1-301.
DR   PDB; 5H01; X-ray; 2.19 A; A/B/C/D=1-301.
DR   PDBsum; 5GZX; -.
DR   PDBsum; 5GZY; -.
DR   PDBsum; 5H00; -.
DR   PDBsum; 5H01; -.
DR   AlphaFoldDB; Q52086; -.
DR   SMR; Q52086; -.
DR   BRENDA; 3.8.1.9; 5092.
DR   GO; GO:0033975; F:(R)-2-haloacid dehalogenase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.20.1290.10; -; 2.
DR   InterPro; IPR019714; 2-haloacid_dehalogenase_DehI.
DR   InterPro; IPR029032; AhpD-like.
DR   Pfam; PF10778; DehI; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase.
FT   CHAIN           1..301
FT                   /note="(R)-2-haloacid dehalogenase"
FT                   /id="PRO_0000079169"
FT   TURN            26..28
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           31..44
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           51..56
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           60..74
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           77..97
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           104..110
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           114..147
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           158..161
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   TURN            179..181
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           184..197
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           204..209
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           213..223
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           224..226
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           230..248
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           258..264
FT                   /evidence="ECO:0007829|PDB:5GZY"
FT   HELIX           268..298
FT                   /evidence="ECO:0007829|PDB:5GZY"
SQ   SEQUENCE   301 AA;  33977 MW;  DA76926AFFFB7E51 CRC64;
     MNLPDNSIHL QLPRPVCEAI IRPVPEHRAD QELSEIYRDL KATFGVPWVG VITQAVAYYR
     PFFAEAWRRF APSAKTHFFE RASDDIRIRS WELMGQSFVI EGQTDRLREM GYSVREIGQI
     RAVLDIFDYG NPKYLIFATA IKEGLLSGRT FGGAAGDARC HFPRSPICQI DPIPVMVEEH
     HAGGTLSQVY ADIKQTLQLP FINSDYKAMA RWPSYLEQAW GALKPCIDTP AYQAGRFDIN
     ARALAALDAL PTAYRMSRDD ALQAGLSEAQ TDELIQVISL FQWMLSGLVL NVTHFKQQAL
     K