ID   HADD_RHISH              Reviewed;         257 AA.
AC   Q8KLS9; Q53186;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=(R)-2-haloacid dehalogenase;
DE            EC=3.8.1.9;
DE   AltName: Full=D-2-haloacid dehalogenase;
DE   AltName: Full=D-DEX;
DE   AltName: Full=D-specific mono chloro propionoic acid dehalogenase;
DE   AltName: Full=DehIII;
GN   Name=dehI; Synonyms=dehD;
OS   Rhizobium sp. (strain NHG3).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=196607;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-8.
RX   PubMed=8654424; DOI=10.1111/j.1432-1033.1996.t01-1-00744.x;
RA   Cairns S.S., Cornish A., Cooper R.A.;
RT   "Cloning, sequencing and expression in Escherichia coli of two Rhizobium
RT   sp. genes encoding haloalkanoate dehalogenases of opposite
RT   stereospecificity.";
RL   Eur. J. Biochem. 235:744-749(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15865161; DOI=10.1007/s10532-004-5670-5;
RA   Higgins T.P., Hope S.J., Effendi A.J., Dawson S.S., Dancer B.N.;
RT   "Biochemical and molecular characterisation of the 2,3-dichloro-1-propanol
RT   dehalogenase and stereospecific haloalkanoic dehalogenases from a versatile
RT   Agrobacterium sp.";
RL   Biodegradation 16:485-492(2005).
CC   -!- FUNCTION: Catalyzes the hydrolytic dehalogenation of small (R)-2-
CC       haloalkanoic acids to yield the corresponding (S)-2-hydroxyalkanoic
CC       acids. Acts on acids of short chain lengths, C(2) to C(4), with
CC       inversion of configuration at C-2.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an (R)-2-haloacid + H2O = a (2S)-2-hydroxycarboxylate + a
CC         halide anion + H(+); Xref=Rhea:RHEA:22188, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:58123,
CC         ChEBI:CHEBI:137406; EC=3.8.1.9;
CC   -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. S-2-
CC       haloalkanoic acid dehalogenase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA63793.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors.; Evidence={ECO:0000305};
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DR   EMBL; X93597; CAA63793.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AJ488572; CAD56483.1; -; Genomic_DNA.
DR   PIR; S62363; S62363.
DR   AlphaFoldDB; Q8KLS9; -.
DR   SMR; Q8KLS9; -.
DR   BRENDA; 3.8.1.9; 206.
DR   GO; GO:0033975; F:(R)-2-haloacid dehalogenase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.20.1290.10; -; 2.
DR   InterPro; IPR029032; AhpD-like.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   CHAIN           1..257
FT                   /note="(R)-2-haloacid dehalogenase"
FT                   /id="PRO_0000079170"
SQ   SEQUENCE   257 AA;  28838 MW;  407B32108A8D08F0 CRC64;
     MIDLPRHPPS MLPVIRTVPE HAATGELKRR YDAVKSAFDV PWMGVVAMAH TQYPRFFDAL
     WEGFEPIAGT RAFQDACRAM RAATEAGVER SLGISPLAHR LQDLGYDPRE IGEIRTIIEV
     FSHGNYPYIL LATVSRYLLS GGDLSGEPQV FETSPRSPHI FHQPILMEPH HADEHTRGIF
     ADIQATLALP ILNTDYRALA RWPSYFHLAW AELRPLIRTP SHAALSQQLH EQAIAVLRTL
     PNPARLKGDM VTRGCGR