ID   HAD_AGRTR               Reviewed;         232 AA.
AC   P60527;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=(S)-2-haloacid dehalogenase {ECO:0000305};
DE            EC=3.8.1.2 {ECO:0000269|PubMed:9425247};
DE   AltName: Full=2-haloalkanoic acid dehalogenase;
DE   AltName: Full=Cryptic L-isomer-specific dehalogenase {ECO:0000303|PubMed:9425247};
DE   AltName: Full=DhlS5I {ECO:0000303|PubMed:9425247};
DE   AltName: Full=Halocarboxylic acid halidohydrolase;
DE   AltName: Full=L-2-haloacid dehalogenase;
DE   AltName: Full=L-DEX;
OS   Agrobacterium tumefaciens (strain RS5).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=260551;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=9425247; DOI=10.1007/s002849900286;
RA   Kohler R., Brokamp A., Schwarze R., Reiting R.H., Schmidt F.R.J.;
RT   "Characteristics and DNA-sequence of a cryptic haloalkanoic acid
RT   dehalogenase from Agrobacterium tumefaciens RS5.";
RL   Curr. Microbiol. 36:96-101(1998).
CC   -!- FUNCTION: Catalyzes the hydrolytic dehalogenation of small (S)-2-
CC       haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic
CC       acids (PubMed:9425247). Acts on acids of short chain lengths, C(2) to
CC       C(4), with inversion of configuration at C-2 (PubMed:9425247). Active
CC       with 2-halogenated carboxylic acids and converts only the S-isomer (or
CC       L-isomer) of 2-chloropropionic acid with inversion of configuration to
CC       produce R-lactate (or D-isomer) (PubMed:9425247).
CC       {ECO:0000269|PubMed:9425247}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an (S)-2-haloacid + H2O = a (2R)-2-hydroxycarboxylate + a
CC         halide anion + H(+); Xref=Rhea:RHEA:11192, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:58314,
CC         ChEBI:CHEBI:137405; EC=3.8.1.2;
CC         Evidence={ECO:0000269|PubMed:9425247};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2-chloropropanoate + H2O = (R)-lactate + chloride + H(+);
CC         Xref=Rhea:RHEA:67956, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16004, ChEBI:CHEBI:17996, ChEBI:CHEBI:73934;
CC         Evidence={ECO:0000269|PubMed:9425247};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Optimum temperature is 65 degrees Celsius.
CC         {ECO:0000269|PubMed:9425247};
CC   -!- BIOTECHNOLOGY: (S)-2-haloacid dehalogenases may be used for the
CC       biodegradation of halogenated substances and their derivatives which
CC       are widely used as pesticides, herbicides and other industrial
CC       products. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. S-2-
CC       haloalkanoic acid dehalogenase family. {ECO:0000305}.
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DR   AlphaFoldDB; P60527; -.
DR   SMR; P60527; -.
DR   GO; GO:0018784; F:(S)-2-haloacid dehalogenase activity; IEA:UniProtKB-EC.
DR   CDD; cd02588; HAD_L2-DEX; 1.
DR   Gene3D; 1.10.150.240; -; 1.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR006328; 2-HAD.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR006439; HAD-SF_hydro_IA.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR023198; PGP-like_dom2.
DR   PRINTS; PR00413; HADHALOGNASE.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   TIGRFAMs; TIGR01493; HAD-SF-IA-v2; 1.
DR   TIGRFAMs; TIGR01428; HAD_type_II; 1.
PE   1: Evidence at protein level;
KW   Hydrolase.
FT   CHAIN           1..232
FT                   /note="(S)-2-haloacid dehalogenase"
FT                   /id="PRO_0000079168"
FT   REGION          175..180
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250|UniProtKB:Q53464"
FT   ACT_SITE        10
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q53464"
FT   BINDING         11..12
FT                   /ligand="an (S)-2-haloacid"
FT                   /ligand_id="ChEBI:CHEBI:137405"
FT                   /evidence="ECO:0000250|UniProtKB:Q53464"
FT   BINDING         41
FT                   /ligand="an (S)-2-haloacid"
FT                   /ligand_id="ChEBI:CHEBI:137405"
FT                   /evidence="ECO:0000250|UniProtKB:Q53464"
FT   BINDING         118..119
FT                   /ligand="an (S)-2-haloacid"
FT                   /ligand_id="ChEBI:CHEBI:137405"
FT                   /evidence="ECO:0000250|UniProtKB:Q53464"
FT   SITE            14
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250|UniProtKB:Q53464"
FT   SITE            151
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250|UniProtKB:Q53464"
FT   SITE            157
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250|UniProtKB:Q53464"
SQ   SEQUENCE   232 AA;  26164 MW;  A618DD4ECF22DBC1 CRC64;
     MKHIKAIAFD LYGTLFDVHS VIDQCEKRFP GRGREVSTLW RQKQLEYTWL RSLMNRYVTF
     EQATEDALRY TCRHLGFALD DAACTVLCDA YLRLQAFPEV PPRLRELRNR GLQLAVLSNG
     SPHSIGAVVG NAGIRDEFDH LISVDPVRVY KPHDRAYGLA EEAFGLARTS ILFVSSNGWD
     ATGARYFGFP TCWINRGGNV FEEMGQTPDW DLLGIDEIVR LFDSAEGSAP SV