HAD_PSEFL
ID HAD_PSEFL Reviewed; 227 AA.
AC Q59666;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 14-AUG-2001, sequence version 2.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=(S)-2-haloacid dehalogenase {ECO:0000305};
DE EC=3.8.1.2 {ECO:0000250|UniProtKB:Q53464};
DE AltName: Full=2-haloalkanoic acid dehalogenase;
DE AltName: Full=Halocarboxylic acid halidohydrolase;
DE AltName: Full=L-2-haloacid dehalogenase;
GN Name=dhl VII;
OS Pseudomonas fluorescens.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=294;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ABVII;
RA Honnens E., Reiting R., Brokamp A., Schmidt F.J.R.;
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the hydrolytic dehalogenation of small (S)-2-
CC haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic
CC acids. Acts on acids of short chain lengths, C(2) to C(4), with
CC inversion of configuration at C-2. Active with 2-halogenated carboxylic
CC acids and converts only the S-isomer (or L-isomer) of 2-chloropropionic
CC acid with inversion of configuration to produce R-lactate (or D-
CC isomer). {ECO:0000250|UniProtKB:Q53464}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an (S)-2-haloacid + H2O = a (2R)-2-hydroxycarboxylate + a
CC halide anion + H(+); Xref=Rhea:RHEA:11192, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:58314,
CC ChEBI:CHEBI:137405; EC=3.8.1.2;
CC Evidence={ECO:0000250|UniProtKB:Q53464};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-2-chloropropanoate + H2O = (R)-lactate + chloride + H(+);
CC Xref=Rhea:RHEA:67956, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16004, ChEBI:CHEBI:17996, ChEBI:CHEBI:73934;
CC Evidence={ECO:0000250|UniProtKB:Q53464};
CC -!- BIOTECHNOLOGY: (S)-2-haloacid dehalogenases may be used for the
CC biodegradation of halogenated substances and their derivatives which
CC are widely used as pesticides, herbicides and other industrial
CC products. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. S-2-
CC haloalkanoic acid dehalogenase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X94147; CAA63861.2; -; Genomic_DNA.
DR AlphaFoldDB; Q59666; -.
DR SMR; Q59666; -.
DR GO; GO:0018784; F:(S)-2-haloacid dehalogenase activity; IEA:UniProtKB-EC.
DR CDD; cd02588; HAD_L2-DEX; 1.
DR Gene3D; 1.10.150.240; -; 1.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR006328; 2-HAD.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006439; HAD-SF_hydro_IA.
DR InterPro; IPR041492; HAD_2.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR023198; PGP-like_dom2.
DR Pfam; PF13419; HAD_2; 1.
DR PRINTS; PR00413; HADHALOGNASE.
DR SUPFAM; SSF56784; SSF56784; 1.
DR TIGRFAMs; TIGR01493; HAD-SF-IA-v2; 1.
DR TIGRFAMs; TIGR01428; HAD_type_II; 1.
PE 3: Inferred from homology;
KW Hydrolase.
FT CHAIN 1..227
FT /note="(S)-2-haloacid dehalogenase"
FT /id="PRO_0000079165"
FT REGION 175..180
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250|UniProtKB:Q53464"
FT ACT_SITE 10
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q53464"
FT BINDING 11..12
FT /ligand="an (S)-2-haloacid"
FT /ligand_id="ChEBI:CHEBI:137405"
FT /evidence="ECO:0000250|UniProtKB:Q53464"
FT BINDING 41
FT /ligand="an (S)-2-haloacid"
FT /ligand_id="ChEBI:CHEBI:137405"
FT /evidence="ECO:0000250|UniProtKB:Q53464"
FT BINDING 118..119
FT /ligand="an (S)-2-haloacid"
FT /ligand_id="ChEBI:CHEBI:137405"
FT /evidence="ECO:0000250|UniProtKB:Q53464"
FT SITE 14
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250|UniProtKB:Q53464"
FT SITE 151
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250|UniProtKB:Q53464"
FT SITE 157
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250|UniProtKB:Q53464"
SQ SEQUENCE 227 AA; 25637 MW; 479D5C29FA91F234 CRC64;
MKNIQGAVFD LYGTLYDVNS VAQVCEEVYS GHGDSISRLW RQKQLEYTWL RSLMGRYVNF
EKATEDALRF TCTHLGLSLD DETHQRLSDA YLHLTPYADT ADALRRLKAA GLPVGIISNG
SHCSIEQVVT NSEMNWAFDQ LISVEDVQVF KPDSRVYSLA EKRMGFPKEN ILFVSSNAWD
ASAASNFGFP VCWINRQNGA FDELDAKPTH VVRNLAEMSN WLVNSLD