ID   HAH4_HALMT              Reviewed;         359 AA.
AC   Q48236; I3RA36;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2012, sequence version 3.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Halocin-H4;
DE   Flags: Precursor;
GN   Name=halH4; OrderedLocusNames=HFX_5264;
OS   Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC
OS   14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei).
OG   Plasmid pHM300.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=523841;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX   PubMed=8990311; DOI=10.1128/jb.179.2.548-551.1997;
RA   Cheung J., Danna K.J., O'Connor E.M., Price L.B., Shand R.F.;
RT   "Isolation, sequence, and expression of the gene encoding halocin H4, a
RT   bacteriocin from the halophilic archaeon Haloferax mediterranei R4.";
RL   J. Bacteriol. 179:548-551(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX   PubMed=22843593; DOI=10.1128/jb.00880-12;
RA   Han J., Zhang F., Hou J., Liu X., Li M., Liu H., Cai L., Zhang B., Chen Y.,
RA   Zhou J., Hu S., Xiang H.;
RT   "Complete genome sequence of the metabolically versatile halophilic
RT   archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-
RT   hydroxyvalerate) producer.";
RL   J. Bacteriol. 194:4463-4464(2012).
RN   [3]
RP   CHARACTERIZATION.
RA   Meseguer I., Rodriguez-Valera F.;
RT   "Production and purification of halocin H4.";
RL   FEMS Microbiol. Lett. 28:177-182(1985).
RN   [4]
RP   CHARACTERIZATION.
RA   Meseguer I., Rodriguez-Valera F.;
RT   "Effect of halocin H4 on cells of Halobacterium halobium.";
RL   J. Gen. Microbiol. 132:3061-3068(1986).
CC   -!- FUNCTION: Has antibacterial activity against other haloarchaeons.
CC       Interacts with the membrane of the target cells where it causes
CC       permeability changes that result in an ionic imbalance leading to cell
CC       lysis and death.
CC   -!- SUBCELLULAR LOCATION: Secreted.
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DR   EMBL; U16389; AAB58316.1; -; Genomic_DNA.
DR   EMBL; CP001870; AFK21096.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q48236; -.
DR   TCDB; 9.A.44.1.1; the archaeocin/halocin h4 (halh4) family.
DR   EnsemblBacteria; AFK21096; AFK21096; HFX_5264.
DR   KEGG; hme:HFX_5264; -.
DR   HOGENOM; CLU_770753_0_0_2; -.
DR   Proteomes; UP000006469; Plasmid pHM300.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing; Plasmid;
KW   Secreted; Signal.
FT   SIGNAL          1..46
FT   CHAIN           47..359
FT                   /note="Halocin-H4"
FT                   /id="PRO_0000013169"
FT   REGION          40..59
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          340..359
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        171
FT                   /note="E -> D (in Ref. 1; AAB58316)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   359 AA;  39639 MW;  61B4D0B564409B3A CRC64;
     MSKDRDGRRT SRRGTLKKIG GFSLGALSFG AVGRTQAATG SSVTTADIAP PGPNGDPKSV
     QIDDKYTGAE MYGEGDFRVG LGTDLTMYPP VYRESLGNGS GGWEFDFTVC GSTACRFVDS
     NGDVKEDDKA KEMWWQEINF NDINQDLYSR NDSDWVGSTP ADTQPEFDYT EFALARDGVT
     LALTALNPAM GSLALGATYF LSDMVNWIAS QHEDDSSLKR KWDYDGLSGP LYADSSTYLL
     ARDEMTSNSY ESFTIDNIAV AFPEFPVRTK YYVTFTAPDD PSTQSISTLE EEGIYRVPAT
     EVAAARPPGS RRSKSAADEM VYVADPKKFI EVEPVKNPSI PDRIYEEIEQ KKKQRSRKQ