HAIR_DROVI
ID HAIR_DROVI Reviewed; 379 AA.
AC P29303; B4LEM2;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2018, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Protein hairy {ECO:0000303|PubMed:1588951};
GN Name=h {ECO:0000303|PubMed:1588951};
OS Drosophila virilis (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila.
OX NCBI_TaxID=7244;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND WRPW MOTIF.
RX PubMed=1588951; DOI=10.1128/mcb.12.6.2475-2483.1992;
RA Wainwright S.M., Ish-Horowicz D.;
RT "Point mutations in the Drosophila hairy gene demonstrate in vivo
RT requirements for basic, helix-loop-helix, and WRPW domains.";
RL Mol. Cell. Biol. 12:2475-2483(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tucson 15010-1051.87;
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- FUNCTION: Pair-rule protein that regulates embryonic segmentation and
CC adult bristle patterning. Transcriptional repressor of genes that
CC require a bHLH protein for their transcription (e.g. ftz).
CC {ECO:0000250|UniProtKB:P14003}.
CC -!- SUBUNIT: Transcription repression requires formation of a complex with
CC a corepressor protein (Groucho). {ECO:0000250|UniProtKB:P14003}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00380,
CC ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- DOMAIN: Has a particular type of basic domain (presence of a helix-
CC interrupting proline) that binds to the N-box (CACNAG), rather than the
CC canonical E-box (CANNTG). {ECO:0000250|UniProtKB:Q26263}.
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC domain necessary for the interaction with Groucho, a transcriptional
CC corepressor recruited to specific target DNA by Hairy-related proteins.
CC {ECO:0000250|UniProtKB:P14003}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M87885; AAA28602.1; -; mRNA.
DR EMBL; CH940647; EDW69107.1; -; Genomic_DNA.
DR PIR; A44443; A44443.
DR RefSeq; XP_002046765.1; XM_002046729.2.
DR AlphaFoldDB; P29303; -.
DR SMR; P29303; -.
DR ELM; P29303; -.
DR STRING; 7244.FBpp0227481; -.
DR EnsemblMetazoa; FBtr0228989; FBpp0227481; FBgn0013115.
DR GeneID; 6623507; -.
DR KEGG; dvi:6623507; -.
DR eggNOG; KOG4304; Eukaryota.
DR HOGENOM; CLU_068550_2_1_1; -.
DR OMA; EQPWRPW; -.
DR OrthoDB; 1427802at2759; -.
DR ChiTaRS; h; fly.
DR Proteomes; UP000008792; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IEA:EnsemblMetazoa.
DR GO; GO:0070888; F:E-box binding; IEA:EnsemblMetazoa.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000902; P:cell morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0007424; P:open tracheal system development; IEA:EnsemblMetazoa.
DR GO; GO:0007366; P:periodic partitioning by pair rule gene; IEA:UniProtKB-KW.
DR GO; GO:0001666; P:response to hypoxia; IEA:EnsemblMetazoa.
DR GO; GO:0007435; P:salivary gland morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0035239; P:tube morphogenesis; IEA:EnsemblMetazoa.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR Pfam; PF07527; Hairy_orange; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00511; ORANGE; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Nucleus; Pair-rule protein;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..379
FT /note="Protein hairy"
FT /id="PRO_0000127183"
FT DOMAIN 38..95
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 114..143
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT REGION 20..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 36..55
FT /note="Interaction with Topors"
FT /evidence="ECO:0000250|UniProtKB:P14003"
FT REGION 167..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 298..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 376..379
FT /note="WRPW motif"
FT /evidence="ECO:0000269|PubMed:1588951"
FT COMPBIAS 32..50
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 186..208
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 298..330
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 29
FT /note="Missing (in Ref. 1; AAA28602)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 379 AA; 41512 MW; AA29E479FAD9096C CRC64;
MVTGITTTMS PNVLGTAVVS TQQQQQQQQH KEAPIKSDRR SNKPIMEKRR RARINNCLNE
LKTLILDATK KDPARHSKLE KADILEKTVK HLQELQRQQA AMQQAADPKI INKFKAGFAD
CANEVSRFPG LDSTQRRRLL QHLSNCINGV KTELHHQQRQ QALAQAQSLH AQVVLPSPPS
SPEQEPSVTP VAASGNNNSS SNNTNTTAPY LFGQIQQNAN GYFLPNGMQV IPTKLPNGSI
ALVLPQSLPQ QQQQQLLQQH QHHQQQQQLA AAAAAAAAAA AAVAQQHQQS PLLVAMPQRT
ASTGSASSHS SAGYESAPSS SSSRGSYAPP SPANSAYEPM DVKPSVIQRV PHMHLEQQPL
SLVIKKQIKV EEEQPWRPW
