HAK1_ORYSJ
ID HAK1_ORYSJ Reviewed; 801 AA.
AC Q6VVA6; A3ATH1; Q0JDI4; Q7XV89; Q8VXC0;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Potassium transporter 1;
DE AltName: Full=OsHAK1;
GN Name=HAK1; OrderedLocusNames=Os04g0401700, LOC_Os04g32920;
GN ORFNames=OsJ_14662, OSJNBb0012E08.7;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Yao Q., Peng R., Xiong A.;
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 26-801 (ISOFORM 2), FUNCTION, TISSUE
RP SPECIFICITY, NOMENCLATURE, AND INDUCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=12376644; DOI=10.1104/pp.007781;
RA Banuelos M.A., Garciadeblas B., Cubero B., Rodriguez-Navarro A.;
RT "Inventory and functional characterization of the HAK potassium
RT transporters of rice.";
RL Plant Physiol. 130:784-795(2002).
RN [8]
RP GENE FAMILY.
RX PubMed=19302972; DOI=10.1016/s1673-8527(08)60103-4;
RA Yang Z., Gao Q., Sun C., Li W., Gu S., Xu C.;
RT "Molecular evolution and functional divergence of HAK potassium transporter
RT gene family in rice (Oryza sativa L.).";
RL J. Genet. Genomics 36:161-172(2009).
CC -!- FUNCTION: High-affinity potassium transporter. Also transports
CC rubidium, with the same affinity and cesium, with a lower affinity.
CC {ECO:0000269|PubMed:12376644}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=6 uM for K(+) (at pH 5.5);
CC KM=6 uM for Rb(+) (at pH 5.5);
CC KM=11 uM for Cs(+) (at pH 5.5);
CC KM=6000 uM for Na(+) (at pH 5.5);
CC Vmax=10 nmol/min/mg enzyme with K(+) as ion;
CC Vmax=10 nmol/min/mg enzyme with Rb(+) as ion;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6VVA6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6VVA6-2; Sequence=VSP_013570;
CC -!- TISSUE SPECIFICITY: Expressed almost exclusively in roots.
CC {ECO:0000269|PubMed:12376644}.
CC -!- INDUCTION: By potassium starvation in roots of young seedlings.
CC {ECO:0000269|PubMed:12376644}.
CC -!- SIMILARITY: Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.
CC {ECO:0000305}.
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DR EMBL; AY324878; AAQ74384.1; -; mRNA.
DR EMBL; AL606610; CAD40783.1; -; Genomic_DNA.
DR EMBL; AP008210; BAF14603.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS89046.1; -; Genomic_DNA.
DR EMBL; CM000141; EAZ30610.1; -; Genomic_DNA.
DR EMBL; AJ427970; CAD20991.1; -; mRNA.
DR RefSeq; XP_015635766.1; XM_015780280.1. [Q6VVA6-2]
DR RefSeq; XP_015635767.1; XM_015780281.1. [Q6VVA6-2]
DR RefSeq; XP_015635769.1; XM_015780283.1.
DR AlphaFoldDB; Q6VVA6; -.
DR STRING; 4530.OS04T0401700-03; -.
DR TCDB; 2.A.72.3.7; the k(+) uptake permease (kup) family.
DR iPTMnet; Q6VVA6; -.
DR PaxDb; Q6VVA6; -.
DR PRIDE; Q6VVA6; -.
DR EnsemblPlants; Os04t0401700-01; Os04t0401700-01; Os04g0401700. [Q6VVA6-2]
DR EnsemblPlants; Os04t0401700-03; Os04t0401700-03; Os04g0401700. [Q6VVA6-2]
DR GeneID; 4335729; -.
DR Gramene; Os04t0401700-01; Os04t0401700-01; Os04g0401700. [Q6VVA6-2]
DR Gramene; Os04t0401700-03; Os04t0401700-03; Os04g0401700. [Q6VVA6-2]
DR KEGG; osa:4335729; -.
DR eggNOG; ENOG502QPSA; Eukaryota.
DR HOGENOM; CLU_008142_2_0_1; -.
DR InParanoid; Q6VVA6; -.
DR OMA; KRIAVNY; -.
DR OrthoDB; 332035at2759; -.
DR BioCyc; MetaCyc:MON-14582; -.
DR SABIO-RK; Q6VVA6; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000007752; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR Genevisible; Q6VVA6; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015079; F:potassium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006813; P:potassium ion transport; IDA:CACAO.
DR InterPro; IPR003855; K+_transporter.
DR PANTHER; PTHR30540; PTHR30540; 1.
DR Pfam; PF02705; K_trans; 1.
DR TIGRFAMs; TIGR00794; kup; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Ion transport; Membrane; Potassium;
KW Potassium transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..801
FT /note="Potassium transporter 1"
FT /id="PRO_0000209090"
FT TOPO_DOM 1..57
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..80
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..94
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..181
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..219
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..247
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..298
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 320..328
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 350..375
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..398
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..429
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 451..461
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 462..482
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 483..487
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 509..511
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 512..532
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 533..801
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 679..728
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 691..718
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 367..375
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12376644"
FT /id="VSP_013570"
FT CONFLICT 43
FT /note="K -> E (in Ref. 1; AAQ74384)"
FT /evidence="ECO:0000305"
FT CONFLICT 689
FT /note="R -> G (in Ref. 1; AAQ74384)"
FT /evidence="ECO:0000305"
FT CONFLICT 771
FT /note="Y -> F (in Ref. 1; AAQ74384)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 801 AA; 88306 MW; 8BBAC5030CAD8F00 CRC64;
MSSALEVEGS GSPGVEPAAT ATASRLKRHD SLFGDAEKVS GGKHHGGSAV SWAVTLHLAF
QSVGIIYGDI GTSPLYVYSS TFPDGIGHRD DLVGVLSLIL YTLIIIPMLK YVFIVLYAND
NGDGGTFALY SLISRYAKIR MIPNQQAEDA MVSNYSIEAP SSQLRRAQWV KHKLESSRAA
KMALFFLTIL GTSMVMGDGT LTPAISVLSA VSGIREKAPN LTQTQVVLIS VAILFMLFSV
QRFGTDKVGY TFAPIISVWF LLIAGIGLYN LVVHEITILK AFNPWYIVQY FRRNGKKGWV
SLGGVVLCVT GTEGMFADLG HFNIRAVQIS FNCILFPSVA LCYIGQAAYL RKFPENVSDT
FYKSIPGKYR DRLNFGPLFW PTFIVAILAA IIASQAMLSG AFAILSKALS LGCLPRVRVI
HTSKKYEGQV YIPEVNFMMG LASIIVTIAF RTTTSIGNAY GICVVTTFMV TTHLMTVVML
LIWKKHLVFI LLFYCVFGFT EVVYLSSILS KFVDGGYLPF CFAMVLMTMM ATWHYVHVRR
YWYELDHIVP TAELASLLEE NGGVRRVPGV GLLYTELVQG IPPLFPRLVR KIPSVHAVFV
FISIKHLPIP HVAAAERFLF RQVGPRARRV FRCVARYGYT DALEEPREFA AFLVDGLKMF
IQEESAFAPH QEMIDAAADD DDEAAARPRR STSSAVHSEE AIQAASSGRT TASSVQLQAG
GEPPAAMDVE EEKRLIDREV GRGVVYLMGE ANVSAGPNSS ILKRIAVNYI YTFLRKNLTE
GHRALAIPND QLLKVGITYE I
