ID   HAP3_KLULA              Reviewed;         205 AA.
AC   P40914;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 124.
DE   RecName: Full=Transcriptional activator HAP3;
GN   Name=HAP3; OrderedLocusNames=KLLA0E10417g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=7845362; DOI=10.1007/bf00279755;
RA   Mulder W., Scholten I.H.J.M., de Boer R.W., Grivell L.A.;
RT   "Sequence of the HAP3 transcription factor of Kluyveromyces lactis predicts
RT   the presence of a novel 4-cysteine zinc-finger motif.";
RL   Mol. Gen. Genet. 245:96-106(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Binds to the upstream activation site (UAS) of the CYC1 gene
CC       and other genes involved in mitochondrial electron transport and
CC       activates their expression. Recognizes the sequence CCAAT.
CC   -!- SUBUNIT: Two complexes bind CCAAT; complex I, that consists of HAP2/3/5
CC       and complex II, that consists of HAP2/3/5/4.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the NFYB/HAP3 subunit family. {ECO:0000305}.
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DR   EMBL; L25779; AAC41662.1; -; Genomic_DNA.
DR   EMBL; CR382125; CAG99508.1; -; Genomic_DNA.
DR   PIR; S51565; S51565.
DR   RefSeq; XP_454421.1; XM_454421.1.
DR   AlphaFoldDB; P40914; -.
DR   SMR; P40914; -.
DR   STRING; 28985.XP_454421.1; -.
DR   EnsemblFungi; CAG99508; CAG99508; KLLA0_E10429g.
DR   GeneID; 2894288; -.
DR   KEGG; kla:KLLA0_E10429g; -.
DR   eggNOG; KOG0869; Eukaryota.
DR   HOGENOM; CLU_066247_9_0_1; -.
DR   InParanoid; P40914; -.
DR   Proteomes; UP000000598; Chromosome E.
DR   GO; GO:0016602; C:CCAAT-binding factor complex; IEA:InterPro.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   Gene3D; 1.10.20.10; -; 1.
DR   InterPro; IPR003958; CBFA_NFYB_domain.
DR   InterPro; IPR009072; Histone-fold.
DR   InterPro; IPR027113; Transc_fact_NFYB/HAP3.
DR   InterPro; IPR003956; Transcrpt_fac_NFYB/HAP3_CS.
DR   PANTHER; PTHR11064; PTHR11064; 1.
DR   Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR   SUPFAM; SSF47113; SSF47113; 1.
DR   PROSITE; PS00685; NFYB_HAP3; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..205
FT                   /note="Transcriptional activator HAP3"
FT                   /id="PRO_0000204631"
FT   DNA_BIND        27..33
FT                   /evidence="ECO:0000250"
FT   REGION          54..65
FT                   /note="Important for subunit interactions"
FT                   /evidence="ECO:0000250"
FT   REGION          126..205
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        126..146
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        149..199
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   205 AA;  23123 MW;  B33C7B6FBE1D7E1A CRC64;
     MDTETEAEKQ ERHNNYLNEL AEQDRWLPIN NVARLMKNTL PATTKVSKDA KECMQECVSE
     FISFVTSEAC DRCTSGKRKT INGEDILLSL HALGFENYAE VLKIYLAKYR QQQAIKNQMM
     YPKEDVEGSY TEEESRAEDA DHVTQERQQP LHPVPSSAST TTSTEVSASE HVQSSSKSTH
     NPAQAQPRIV VQQPLGSRHS TADHL