ID   HARB1_BOVIN             Reviewed;         349 AA.
AC   Q17QR8;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Putative nuclease HARBI1;
DE            EC=3.1.-.-;
DE   AltName: Full=Harbinger transposase-derived nuclease;
GN   Name=HARBI1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Thalamus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   TISSUE SPECIFICITY.
RX   PubMed=15169610; DOI=10.1089/104454904323090949;
RA   Kapitonov V.V., Jurka J.;
RT   "Harbinger transposons and an ancient HARBI1 gene derived from a
RT   transposase.";
RL   DNA Cell Biol. 23:311-324(2004).
CC   -!- FUNCTION: Transposase-derived protein that may have nuclease activity
CC       (Potential). Does not have transposase activity (By similarity).
CC       {ECO:0000250, ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000305};
CC   -!- SUBUNIT: Interacts with NAIF1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC       Note=Interaction with NAIF1 promotes translocation to the nucleus.
CC       {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Detected in brain. {ECO:0000269|PubMed:15169610}.
CC   -!- SIMILARITY: Belongs to the HARBI1 family. {ECO:0000305}.
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DR   EMBL; BC118217; AAI18218.1; -; mRNA.
DR   RefSeq; NP_001069136.1; NM_001075668.1.
DR   RefSeq; XP_005216510.1; XM_005216453.3.
DR   RefSeq; XP_005216511.1; XM_005216454.3.
DR   AlphaFoldDB; Q17QR8; -.
DR   STRING; 9913.ENSBTAP00000006085; -.
DR   PaxDb; Q17QR8; -.
DR   PRIDE; Q17QR8; -.
DR   Ensembl; ENSBTAT00000006085; ENSBTAP00000006085; ENSBTAG00000004639.
DR   GeneID; 514442; -.
DR   KEGG; bta:514442; -.
DR   CTD; 283254; -.
DR   VEuPathDB; HostDB:ENSBTAG00000004639; -.
DR   VGNC; VGNC:29752; HARBI1.
DR   eggNOG; KOG4585; Eukaryota.
DR   GeneTree; ENSGT00940000154348; -.
DR   HOGENOM; CLU_018552_13_0_1; -.
DR   InParanoid; Q17QR8; -.
DR   OMA; SAETHWP; -.
DR   OrthoDB; 698280at2759; -.
DR   TreeFam; TF327972; -.
DR   Proteomes; UP000009136; Chromosome 15.
DR   Bgee; ENSBTAG00000004639; Expressed in caput epididymis and 105 other tissues.
DR   GO; GO:0034451; C:centriolar satellite; IEA:Ensembl.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR045249; HARBI1-like.
DR   InterPro; IPR026103; HARBI1_animal.
DR   InterPro; IPR027806; HARBI1_dom.
DR   PANTHER; PTHR22930; PTHR22930; 1.
DR   Pfam; PF13359; DDE_Tnp_4; 1.
DR   PRINTS; PR02086; PUTNUCHARBI1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Metal-binding; Nuclease; Nucleus; Reference proteome.
FT   CHAIN           1..349
FT                   /note="Putative nuclease HARBI1"
FT                   /id="PRO_0000263613"
FT   DOMAIN          148..300
FT                   /note="DDE Tnp4"
FT                   /evidence="ECO:0000255"
FT   BINDING         149
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         199
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         225
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         261
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   349 AA;  39003 MW;  BD618E2CAE4DBC1D CRC64;
     MAIPITVLDC DLLLYGRGHR TLDRFKLDDV TDEYLMSMYG FPRQFIYYLV ELLGASLSRP
     TQRSRAISPE TQILAALGFY TSGSFQTRMG DAIGISQASM SRCVANVTEA LVERASQFIH
     FPADEASVQA LKDEFYGLAG IPGVIGVVDC MHVAIKAPNA EDLSYVNRKG LHSLNCLMVC
     DIRGALMTVE TSWPGSLQDC VVLQQSSLSS QFEAGMHKES WLLGDSSFFL RTWLMTPLHI
     PETPAEYRYN MAHSATHSVI EKTFRTLCSR FRCLDGSKGA LQYSPEKSSH IILACCVLHN
     ISLEHGMDVW SSPVTGPVEQ PPEEEYEHME SLDLEADRIR QELMLTHFS