ID   HARB1_DANRE             Reviewed;         349 AA.
AC   Q6AZB8; Q498Q5;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Putative nuclease HARBI1;
DE            EC=3.1.-.-;
DE   AltName: Full=Harbinger transposase-derived nuclease;
GN   Name=harbi1; ORFNames=zgc:91866;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=SJD;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   TISSUE SPECIFICITY.
RX   PubMed=15169610; DOI=10.1089/104454904323090949;
RA   Kapitonov V.V., Jurka J.;
RT   "Harbinger transposons and an ancient HARBI1 gene derived from a
RT   transposase.";
RL   DNA Cell Biol. 23:311-324(2004).
CC   -!- FUNCTION: Transposase-derived protein that may have nuclease activity
CC       (Potential). Does not have transposase activity (By similarity).
CC       {ECO:0000250, ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HARBI1 family. {ECO:0000305}.
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DR   EMBL; BC078390; AAH78390.1; -; mRNA.
DR   EMBL; BC100116; AAI00117.1; -; mRNA.
DR   RefSeq; NP_001003734.1; NM_001003734.1.
DR   AlphaFoldDB; Q6AZB8; -.
DR   STRING; 7955.ENSDARP00000108392; -.
DR   PaxDb; Q6AZB8; -.
DR   PRIDE; Q6AZB8; -.
DR   Ensembl; ENSDART00000052323; ENSDARP00000052322; ENSDARG00000036038.
DR   Ensembl; ENSDART00000190979; ENSDARP00000145132; ENSDARG00000036038.
DR   GeneID; 445279; -.
DR   KEGG; dre:445279; -.
DR   CTD; 283254; -.
DR   ZFIN; ZDB-GENE-040608-1; harbi1.
DR   eggNOG; KOG4585; Eukaryota.
DR   GeneTree; ENSGT00940000154348; -.
DR   HOGENOM; CLU_018552_13_0_1; -.
DR   InParanoid; Q6AZB8; -.
DR   OMA; SAETHWP; -.
DR   OrthoDB; 698280at2759; -.
DR   PhylomeDB; Q6AZB8; -.
DR   TreeFam; TF327972; -.
DR   PRO; PR:Q6AZB8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 7.
DR   Bgee; ENSDARG00000036038; Expressed in mature ovarian follicle and 26 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR045249; HARBI1-like.
DR   InterPro; IPR026103; HARBI1_animal.
DR   InterPro; IPR027806; HARBI1_dom.
DR   PANTHER; PTHR22930; PTHR22930; 1.
DR   Pfam; PF13359; DDE_Tnp_4; 1.
DR   PRINTS; PR02086; PUTNUCHARBI1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Metal-binding; Nuclease; Nucleus; Reference proteome.
FT   CHAIN           1..349
FT                   /note="Putative nuclease HARBI1"
FT                   /id="PRO_0000263616"
FT   DOMAIN          148..301
FT                   /note="DDE Tnp4"
FT                   /evidence="ECO:0000255"
FT   BINDING         149
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         199
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         226
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         262
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        43
FT                   /note="R -> H (in Ref. 1; AAI00117)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        243
FT                   /note="E -> A (in Ref. 1; AAI00117)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   349 AA;  39329 MW;  D8D9D97A2C4CE50E CRC64;
     MALSIAILDC DLLLHGRGHK TLDRFDIETV SDDFLLNTFG FPREFIYYLV ELLKDSLLRR
     TQRSRAISPD VQILAALGFY TSGSFQSKMG DAIGISQASM SRCVSNVTKA LIEKAPEFIG
     FTRDEATKQQ FKDEFYRIAG IPNVTGVVDC AHIAIKAPNA DDSSYVNKKG FHSINCQLVC
     DARGLLLSAE THWPGSLTDR AVFKQSNVAK LFEEQENDDE GWLLGDNRYP LKKWLMTPVQ
     SPESPADYRY NLAHTTTHEI VDRTFRAIQT RFRCLDGAKG YLQYSPEKCS HIIQACCVLH
     NISLQSGLDA WTFERTEATD QSGEDIDPSD TDDPEALRVR QELIQNHFS