HARB1_HUMAN
ID HARB1_HUMAN Reviewed; 349 AA.
AC Q96MB7; D3DQP9;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Putative nuclease HARBI1;
DE EC=3.1.-.-;
DE AltName: Full=Harbinger transposase-derived nuclease;
GN Name=HARBI1; Synonyms=C11orf77;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, COFACTOR, AND TISSUE SPECIFICITY.
RX PubMed=15169610; DOI=10.1089/104454904323090949;
RA Kapitonov V.V., Jurka J.;
RT "Harbinger transposons and an ancient HARBI1 gene derived from a
RT transposase.";
RL DNA Cell Biol. 23:311-324(2004).
RN [5]
RP FUNCTION, INTERACTION WITH NAIF1, AND SUBCELLULAR LOCATION.
RX PubMed=18339812; DOI=10.1073/pnas.0707746105;
RA Sinzelle L., Kapitonov V.V., Grzela D.P., Jursch T., Jurka J., Izsvak Z.,
RA Ivics Z.;
RT "Transposition of a reconstructed Harbinger element in human cells and
RT functional homology with two transposon-derived cellular genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:4715-4720(2008).
CC -!- FUNCTION: Transposase-derived protein that may have nuclease activity
CC (Potential). Does not have transposase activity.
CC {ECO:0000269|PubMed:15169610, ECO:0000269|PubMed:18339812,
CC ECO:0000305}.
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000305};
CC -!- SUBUNIT: Interacts with NAIF1. {ECO:0000269|PubMed:18339812}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18339812}. Cytoplasm
CC {ECO:0000269|PubMed:18339812}. Note=Interaction with NAIF1 promotes
CC translocation to the nucleus.
CC -!- TISSUE SPECIFICITY: Detected in brain, eye, nerve tissue, kidney and
CC lung. {ECO:0000269|PubMed:15169610}.
CC -!- SIMILARITY: Belongs to the HARBI1 family. {ECO:0000305}.
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DR EMBL; AK057237; BAB71391.1; -; mRNA.
DR EMBL; CH471064; EAW67992.1; -; Genomic_DNA.
DR EMBL; CH471064; EAW67993.1; -; Genomic_DNA.
DR EMBL; CH471064; EAW67994.1; -; Genomic_DNA.
DR EMBL; BC036925; AAH36925.1; -; mRNA.
DR CCDS; CCDS7920.1; -.
DR RefSeq; NP_776172.1; NM_173811.3.
DR RefSeq; XP_016873093.1; XM_017017604.1.
DR RefSeq; XP_016873094.1; XM_017017605.1.
DR RefSeq; XP_016873095.1; XM_017017606.1.
DR AlphaFoldDB; Q96MB7; -.
DR SMR; Q96MB7; -.
DR BioGRID; 129511; 1.
DR IntAct; Q96MB7; 1.
DR STRING; 9606.ENSP00000317743; -.
DR iPTMnet; Q96MB7; -.
DR PhosphoSitePlus; Q96MB7; -.
DR BioMuta; HARBI1; -.
DR DMDM; 74732341; -.
DR jPOST; Q96MB7; -.
DR MassIVE; Q96MB7; -.
DR MaxQB; Q96MB7; -.
DR PaxDb; Q96MB7; -.
DR PeptideAtlas; Q96MB7; -.
DR PRIDE; Q96MB7; -.
DR ProteomicsDB; 77328; -.
DR Antibodypedia; 42871; 98 antibodies from 19 providers.
DR DNASU; 283254; -.
DR Ensembl; ENST00000326737.3; ENSP00000317743.3; ENSG00000180423.4.
DR GeneID; 283254; -.
DR KEGG; hsa:283254; -.
DR MANE-Select; ENST00000326737.3; ENSP00000317743.3; NM_173811.4; NP_776172.1.
DR UCSC; uc001ncy.4; human.
DR CTD; 283254; -.
DR GeneCards; HARBI1; -.
DR HGNC; HGNC:26522; HARBI1.
DR HPA; ENSG00000180423; Low tissue specificity.
DR MIM; 615086; gene.
DR neXtProt; NX_Q96MB7; -.
DR OpenTargets; ENSG00000180423; -.
DR PharmGKB; PA162390577; -.
DR VEuPathDB; HostDB:ENSG00000180423; -.
DR eggNOG; KOG4585; Eukaryota.
DR GeneTree; ENSGT00940000154348; -.
DR HOGENOM; CLU_018552_13_0_1; -.
DR InParanoid; Q96MB7; -.
DR OMA; SAETHWP; -.
DR OrthoDB; 698280at2759; -.
DR PhylomeDB; Q96MB7; -.
DR TreeFam; TF327972; -.
DR PathwayCommons; Q96MB7; -.
DR SignaLink; Q96MB7; -.
DR BioGRID-ORCS; 283254; 15 hits in 1083 CRISPR screens.
DR ChiTaRS; HARBI1; human.
DR GenomeRNAi; 283254; -.
DR Pharos; Q96MB7; Tdark.
DR PRO; PR:Q96MB7; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q96MB7; protein.
DR Bgee; ENSG00000180423; Expressed in sperm and 114 other tissues.
DR ExpressionAtlas; Q96MB7; baseline and differential.
DR Genevisible; Q96MB7; HS.
DR GO; GO:0034451; C:centriolar satellite; IDA:HPA.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR InterPro; IPR045249; HARBI1-like.
DR InterPro; IPR026103; HARBI1_animal.
DR InterPro; IPR027806; HARBI1_dom.
DR PANTHER; PTHR22930; PTHR22930; 1.
DR Pfam; PF13359; DDE_Tnp_4; 1.
DR PRINTS; PR02086; PUTNUCHARBI1.
PE 1: Evidence at protein level;
KW Cytoplasm; Hydrolase; Metal-binding; Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..349
FT /note="Putative nuclease HARBI1"
FT /id="PRO_0000263614"
FT DOMAIN 148..300
FT /note="DDE Tnp4"
FT /evidence="ECO:0000255"
FT BINDING 149
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
FT BINDING 199
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
FT BINDING 225
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
FT BINDING 261
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
SQ SEQUENCE 349 AA; 39146 MW; C424FDE6825A69F2 CRC64;
MAIPITVLDC DLLLYGRGHR TLDRFKLDDV TDEYLMSMYG FPRQFIYYLV ELLGANLSRP
TQRSRAISPE TQVLAALGFY TSGSFQTRMG DAIGISQASM SRCVANVTEA LVERASQFIR
FPADEASIQA LKDEFYGLAG MPGVMGVVDC IHVAIKAPNA EDLSYVNRKG LHSLNCLMVC
DIRGTLMTVE TNWPGSLQDC AVLQQSSLSS QFEAGMHKDS WLLGDSSFFL RTWLMTPLHI
PETPAEYRYN MAHSATHSVI EKTFRTLCSR FRCLDGSKGA LQYSPEKSSH IILACCVLHN
ISLEHGMDVW SSPMTGPMEQ PPEEEYEHME SLDLEADRIR QELMLTHFS
