HARB1_MOUSE
ID HARB1_MOUSE Reviewed; 349 AA.
AC Q8BR93; A2AH19; B7ZCF8; Q8BNA8; Q8BNT1; Q8C9N1;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Putative nuclease HARBI1;
DE EC=3.1.-.-;
DE AltName: Full=Harbinger transposase-derived nuclease;
GN Name=Harbi1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Amnion, Brain cortex, Eye, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=15169610; DOI=10.1089/104454904323090949;
RA Kapitonov V.V., Jurka J.;
RT "Harbinger transposons and an ancient HARBI1 gene derived from a
RT transposase.";
RL DNA Cell Biol. 23:311-324(2004).
CC -!- FUNCTION: Transposase-derived protein that may have nuclease activity
CC (Potential). Does not have transposase activity (By similarity).
CC {ECO:0000250, ECO:0000305}.
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000305};
CC -!- SUBUNIT: Interacts with NAIF1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC Note=Interaction with NAIF1 promotes translocation to the nucleus.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8BR93-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BR93-2; Sequence=VSP_021877, VSP_021878;
CC Name=3;
CC IsoId=Q8BR93-3; Sequence=VSP_021876, VSP_021879;
CC -!- TISSUE SPECIFICITY: Detected in adult brain, eye, nerve tissue and
CC lung. Detected in embryo. {ECO:0000269|PubMed:15169610}.
CC -!- SIMILARITY: Belongs to the HARBI1 family. {ECO:0000305}.
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DR EMBL; AK041747; BAC31051.1; -; mRNA.
DR EMBL; AK045343; BAC32318.1; -; mRNA.
DR EMBL; AK080671; BAC37974.1; -; mRNA.
DR EMBL; AK084226; BAC39145.1; -; mRNA.
DR EMBL; AK147045; BAE27631.1; -; mRNA.
DR EMBL; AL714023; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC094315; AAH94315.1; -; mRNA.
DR CCDS; CCDS50642.1; -. [Q8BR93-1]
DR CCDS; CCDS89518.1; -. [Q8BR93-2]
DR RefSeq; NP_848839.2; NM_178724.4. [Q8BR93-1]
DR RefSeq; XP_006499533.1; XM_006499470.1.
DR RefSeq; XP_006499534.1; XM_006499471.2. [Q8BR93-1]
DR RefSeq; XP_017173596.1; XM_017318107.1. [Q8BR93-2]
DR AlphaFoldDB; Q8BR93; -.
DR STRING; 10090.ENSMUSP00000088098; -.
DR PhosphoSitePlus; Q8BR93; -.
DR EPD; Q8BR93; -.
DR MaxQB; Q8BR93; -.
DR PaxDb; Q8BR93; -.
DR PRIDE; Q8BR93; -.
DR ProteomicsDB; 270886; -. [Q8BR93-1]
DR ProteomicsDB; 270887; -. [Q8BR93-2]
DR ProteomicsDB; 270888; -. [Q8BR93-3]
DR Antibodypedia; 42871; 98 antibodies from 19 providers.
DR DNASU; 241547; -.
DR Ensembl; ENSMUST00000090608; ENSMUSP00000088098; ENSMUSG00000027243. [Q8BR93-1]
DR Ensembl; ENSMUST00000111322; ENSMUSP00000106954; ENSMUSG00000027243. [Q8BR93-2]
DR Ensembl; ENSMUST00000142692; ENSMUSP00000121420; ENSMUSG00000027243. [Q8BR93-3]
DR GeneID; 241547; -.
DR KEGG; mmu:241547; -.
DR UCSC; uc008kwo.1; mouse. [Q8BR93-3]
DR UCSC; uc008kwp.1; mouse. [Q8BR93-1]
DR CTD; 283254; -.
DR MGI; MGI:2443194; Harbi1.
DR VEuPathDB; HostDB:ENSMUSG00000027243; -.
DR eggNOG; KOG4585; Eukaryota.
DR GeneTree; ENSGT00940000154348; -.
DR HOGENOM; CLU_018552_13_0_1; -.
DR InParanoid; Q8BR93; -.
DR OMA; SAETHWP; -.
DR OrthoDB; 698280at2759; -.
DR PhylomeDB; Q8BR93; -.
DR TreeFam; TF327972; -.
DR BioGRID-ORCS; 241547; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Harbi1; mouse.
DR PRO; PR:Q8BR93; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8BR93; protein.
DR Bgee; ENSMUSG00000027243; Expressed in dorsal pancreas and 217 other tissues.
DR ExpressionAtlas; Q8BR93; baseline and differential.
DR Genevisible; Q8BR93; MM.
DR GO; GO:0034451; C:centriolar satellite; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR InterPro; IPR045249; HARBI1-like.
DR InterPro; IPR026103; HARBI1_animal.
DR InterPro; IPR027806; HARBI1_dom.
DR PANTHER; PTHR22930; PTHR22930; 1.
DR Pfam; PF13359; DDE_Tnp_4; 1.
DR PRINTS; PR02086; PUTNUCHARBI1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Hydrolase; Metal-binding; Nuclease;
KW Nucleus; Reference proteome.
FT CHAIN 1..349
FT /note="Putative nuclease HARBI1"
FT /id="PRO_0000263615"
FT DOMAIN 149..300
FT /note="DDE Tnp4"
FT /evidence="ECO:0000255"
FT BINDING 149
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
FT BINDING 199
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
FT BINDING 225
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
FT BINDING 261
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000255"
FT VAR_SEQ 225..246
FT /note="DSSFFLRSWLLTPLPIPETAAE -> KFASINFQFMSIEKLCRRVGEI (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021876"
FT VAR_SEQ 225..245
FT /note="DSSFFLRSWLLTPLPIPETAA -> CATQALLYDCSVRESSAPES (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021877"
FT VAR_SEQ 246..349
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021878"
FT VAR_SEQ 247..349
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021879"
FT CONFLICT 272
FT /note="R -> Q (in Ref. 1; BAC37974)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 349 AA; 38713 MW; BA1CCEB600A46010 CRC64;
MAIPITVLDC DLLLYGRGHR TLDRFKLDDV TDEYLMSMYG FPRQFIYFLV ELLGASLSRP
TQRSRAISPE TQILAALGFY TSGSFQTRMG DAIGISQASM SRCVANVTEA LVERASQFIH
FPVDEAAVQS LKDEFYGLAG MPGVIGVADC IHVAIKAPNA EDLSYVNRKG LHSLNCLVVC
DIRGALMTVE TSWPGSLQDC AVLQRSSLTS QFETGMPKDS WLLGDSSFFL RSWLLTPLPI
PETAAEYRYN RAHSATHSVI ERTLQTLCCR FRCLDGSKGA LQYSPEKCSH IILACCVLHN
ISLDHGMDVW SSPVPGPIDQ PPEGEDEHME SLDLEADRIR QELILTHFS
