ID   HARB1_RAT               Reviewed;         349 AA.
AC   B0BN95;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Putative nuclease HARBI1;
DE            EC=3.1.-.-;
DE   AltName: Full=Harbinger transposase-derived nuclease;
GN   Name=Harbi1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Spleen;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Transposase-derived protein that may have nuclease activity
CC       (Potential). Does not have transposase activity (By similarity).
CC       {ECO:0000250, ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000305};
CC   -!- SUBUNIT: Interacts with NAIF1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC       Note=Interaction with NAIF1 promotes translocation to the nucleus.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HARBI1 family. {ECO:0000305}.
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DR   EMBL; BC158734; AAI58735.1; -; mRNA.
DR   RefSeq; NP_001107265.2; NM_001113793.3.
DR   AlphaFoldDB; B0BN95; -.
DR   STRING; 10116.ENSRNOP00000061152; -.
DR   PaxDb; B0BN95; -.
DR   GeneID; 690164; -.
DR   KEGG; rno:690164; -.
DR   UCSC; RGD:1584007; rat.
DR   CTD; 283254; -.
DR   RGD; 1584007; Harbi1.
DR   eggNOG; KOG4585; Eukaryota.
DR   HOGENOM; CLU_018552_13_0_1; -.
DR   InParanoid; B0BN95; -.
DR   PhylomeDB; B0BN95; -.
DR   TreeFam; TF327972; -.
DR   PRO; PR:B0BN95; -.
DR   Proteomes; UP000002494; Unplaced.
DR   Genevisible; B0BN95; RN.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR045249; HARBI1-like.
DR   InterPro; IPR026103; HARBI1_animal.
DR   InterPro; IPR027806; HARBI1_dom.
DR   PANTHER; PTHR22930; PTHR22930; 1.
DR   Pfam; PF13359; DDE_Tnp_4; 1.
DR   PRINTS; PR02086; PUTNUCHARBI1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Metal-binding; Nuclease; Nucleus; Reference proteome.
FT   CHAIN           1..349
FT                   /note="Putative nuclease HARBI1"
FT                   /id="PRO_0000345621"
FT   DOMAIN          148..300
FT                   /note="DDE Tnp4"
FT                   /evidence="ECO:0000255"
FT   BINDING         149
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         199
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         225
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
FT   BINDING         261
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   349 AA;  38741 MW;  9E8A53B8F7D9BD86 CRC64;
     MAIPITVLDC DLLLYGRGHR TLDRFKLDDV TDEYLMSMYG FPRQFIYYLV ELLGASLSRP
     TQRSRAISPE TQILAALGFY TSGSFQTRMG DAIGISQASM SRCVANVTEA LVERASQFIH
     FPADEAAIQS LKDEFYGLAG MPGVIGAVDC IHVAIKAPNA EDLSYVNRKG LHSLNCLVVC
     DIRGALMTVE TSWPGSLQDC AVLQQSSLSS QFETGMPKDS WLLGDSSFFL HTWLLTPLHI
     PETPAEYRYN RAHSATHSVI EKTLRTLCCR FRCLDGSKGA LQYSPEKSSH IILACCVLHN
     ISLEHGMDVW SSPVTGPIEQ PPEGEDEQME SLDLEADRIR QELILTHFS