HAT5_ARATH
ID HAT5_ARATH Reviewed; 272 AA.
AC Q02283;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Homeobox-leucine zipper protein HAT5;
DE AltName: Full=HD-ZIP protein ATHB-1;
DE AltName: Full=Homeodomain transcription factor ATHB-1;
DE AltName: Full=Homeodomain-leucine zipper protein HAT5;
DE Short=HD-ZIP protein 5;
GN Name=HAT5; Synonyms=ATHB-1; OrderedLocusNames=At3g01470; ORFNames=F4P13.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=1675603; DOI=10.1002/j.1460-2075.1991.tb07703.x;
RA Ruberti I., Sessa G., Lucchetti S., Morelli G.;
RT "A novel class of plant proteins containing a homeodomain with a closely
RT linked leucine zipper motif.";
RL EMBO J. 10:1787-1791(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 64-163.
RC STRAIN=cv. Columbia;
RX PubMed=1349174; DOI=10.1073/pnas.89.9.3894;
RA Schena M., Davis R.W.;
RT "HD-Zip proteins: members of an Arabidopsis homeodomain protein
RT superfamily.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:3894-3898(1992).
RN [6]
RP DNA-BINDING.
RX PubMed=8253077; DOI=10.1002/j.1460-2075.1993.tb06025.x;
RA Sessa G., Morelli G., Ruberti I.;
RT "The Athb-1 and -2 HD-Zip domains homodimerize forming complexes of
RT different DNA binding specificities.";
RL EMBO J. 12:3507-3517(1993).
RN [7]
RP FUNCTION.
RX PubMed=8535134; DOI=10.2307/3870186;
RA Aoyama T., Dong C.-H., Wu Y., Carabelli M., Sessa G., Ruberti I.,
RA Morelli G., Chua N.-H.;
RT "Ectopic expression of the Arabidopsis transcriptional activator Athb-1
RT alters leaf cell fate in tobacco.";
RL Plant Cell 7:1773-1785(1995).
RN [8]
RP DNA-BINDING, AND MUTAGENESIS OF ARG-119.
RX PubMed=9405140; DOI=10.1006/jmbi.1997.1408;
RA Sessa G., Morelli G., Ruberti I.;
RT "DNA-binding specificity of the homeodomain-leucine zipper domain.";
RL J. Mol. Biol. 274:303-309(1997).
RN [9]
RP GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=16055682; DOI=10.1104/pp.105.063461;
RA Henriksson E., Olsson A.S.B., Johannesson H., Johansson H., Hanson J.,
RA Engstroem P., Soederman E.;
RT "Homeodomain leucine zipper class I genes in Arabidopsis. Expression
RT patterns and phylogenetic relationships.";
RL Plant Physiol. 139:509-518(2005).
RN [10]
RP INTERACTION WITH TBP2.
RX PubMed=24531799; DOI=10.1007/s00299-014-1576-9;
RA Capella M., Re D.A., Arce A.L., Chan R.L.;
RT "Plant homeodomain-leucine zipper I transcription factors exhibit different
RT functional AHA motifs that selectively interact with TBP or/and TFIIB.";
RL Plant Cell Rep. 33:955-967(2014).
CC -!- FUNCTION: Probable transcription activator involved in leaf
CC development. Binds to the DNA sequence 5'-CAAT[AT]ATTG-3'.
CC {ECO:0000269|PubMed:8535134}.
CC -!- SUBUNIT: Interacts with DNA as homodimer (PubMed:9405140). Interacts
CC with TBP2 (PubMed:24531799). {ECO:0000269|PubMed:24531799,
CC ECO:0000269|PubMed:9405140}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:16055682}.
CC -!- SIMILARITY: Belongs to the HD-ZIP homeobox family. Class I subfamily.
CC {ECO:0000305}.
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DR EMBL; X58821; CAA41625.1; -; mRNA.
DR EMBL; AC009325; AAF01532.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE73670.1; -; Genomic_DNA.
DR EMBL; AY058188; AAL25601.1; -; mRNA.
DR EMBL; AY098972; AAM19982.1; -; mRNA.
DR EMBL; M90416; AAA32816.1; -; mRNA.
DR PIR; S16325; S16325.
DR RefSeq; NP_186796.1; NM_111013.4.
DR AlphaFoldDB; Q02283; -.
DR SMR; Q02283; -.
DR BioGRID; 6471; 5.
DR IntAct; Q02283; 1.
DR STRING; 3702.AT3G01470.1; -.
DR PaxDb; Q02283; -.
DR PRIDE; Q02283; -.
DR ProteomicsDB; 230308; -.
DR EnsemblPlants; AT3G01470.1; AT3G01470.1; AT3G01470.
DR GeneID; 821138; -.
DR Gramene; AT3G01470.1; AT3G01470.1; AT3G01470.
DR KEGG; ath:AT3G01470; -.
DR Araport; AT3G01470; -.
DR TAIR; locus:2084228; AT3G01470.
DR eggNOG; KOG0483; Eukaryota.
DR HOGENOM; CLU_060842_2_0_1; -.
DR InParanoid; Q02283; -.
DR OMA; QVPEANP; -.
DR OrthoDB; 1066903at2759; -.
DR PhylomeDB; Q02283; -.
DR PRO; PR:Q02283; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q02283; baseline and differential.
DR Genevisible; Q02283; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR GO; GO:0009965; P:leaf morphogenesis; IMP:TAIR.
DR GO; GO:0017148; P:negative regulation of translation; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0009637; P:response to blue light; IEP:TAIR.
DR GO; GO:0009651; P:response to salt stress; IEP:TAIR.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR045224; HDZip_class_I_plant.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR000047; HTH_motif.
DR InterPro; IPR003106; Leu_zip_homeo.
DR PANTHER; PTHR24326; PTHR24326; 1.
DR Pfam; PF02183; HALZ; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..272
FT /note="Homeobox-leucine zipper protein HAT5"
FT /id="PRO_0000048902"
FT DNA_BIND 65..124
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 125..160
FT /note="Leucine-zipper"
FT REGION 158..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..177
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 119
FT /note="R->K: Abolishes DNA-binding."
FT /evidence="ECO:0000269|PubMed:9405140"
SQ SEQUENCE 272 AA; 30933 MW; B85ACCA20EDFA094 CRC64;
MESNSFFFDP SASHGNSMFF LGNLNPVVQG GGARSMMNME ETSKRRPFFS SPEDLYDDDF
YDDQLPEKKR RLTTEQVHLL EKSFETENKL EPERKTQLAK KLGLQPRQVA VWFQNRRARW
KTKQLERDYD LLKSTYDQLL SNYDSIVMDN DKLRSEVTSL TEKLQGKQET ANEPPGQVPE
PNQLDPVYIN AAAIKTEDRL SSGSVGSAVL DDDAPQLLDS CDSYFPSIVP IQDNSNASDH
DNDRSCFADV FVPTTSPSHD HHGESLAFWG WP
