HATB_DICDI
ID HATB_DICDI Reviewed; 118 AA.
AC P42526; Q54SW6;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Hisactophilin-2;
DE AltName: Full=Histidine-rich actin-binding protein 2;
DE Short=HS II;
GN Name=hatB; ORFNames=DDB_G0282143;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, PHOSPHORYLATION, AND
RP MYRISTOYLATION AT GLY-2.
RC STRAIN=AX2, and AX3;
RX PubMed=7822284; DOI=10.1074/jbc.270.2.596;
RA Hanakam F., Eckerskorn C., Lottspeich F., Mueller-Taubenberger A.,
RA Schaefer W., Gerisch G.;
RT "The pH-sensitive actin-binding protein hisactophilin of Dictyostelium
RT exists in two isoforms which both are myristoylated and distributed between
RT plasma membrane and cytoplasm.";
RL J. Biol. Chem. 270:596-602(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA Soldati T.;
RT "Proteomics fingerprinting of phagosome maturation and evidence for the
RT role of a Galpha during uptake.";
RL Mol. Cell. Proteomics 5:2228-2243(2006).
CC -!- FUNCTION: May act as an intracellular pH sensor that links chemotactic
CC signals to responses in the microfilament system of the cells by
CC nucleating actin polymerization or stabilizing the filaments.
CC -!- SUBUNIT: Homodimer or heterodimer of hatA and hatB, linked by a
CC disulfide bond.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Lipid-anchor;
CC Cytoplasmic side.
CC -!- PTM: Phosphorylated. {ECO:0000269|PubMed:7822284}.
CC -!- SIMILARITY: Belongs to the hisactophilin family. {ECO:0000305}.
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DR EMBL; U13671; AAA66208.1; -; mRNA.
DR EMBL; AAFI02000045; EAL66351.1; -; Genomic_DNA.
DR RefSeq; XP_640343.1; XM_635251.1.
DR AlphaFoldDB; P42526; -.
DR SMR; P42526; -.
DR STRING; 44689.DDB0215336; -.
DR iPTMnet; P42526; -.
DR PaxDb; P42526; -.
DR PRIDE; P42526; -.
DR EnsemblProtists; EAL66351; EAL66351; DDB_G0282143.
DR GeneID; 8623444; -.
DR KEGG; ddi:DDB_G0282143; -.
DR dictyBase; DDB_G0282143; hatB.
DR eggNOG; ENOG502RI2J; Eukaryota.
DR HOGENOM; CLU_2077490_0_0_1; -.
DR InParanoid; P42526; -.
DR OMA; THERGHD; -.
DR PhylomeDB; P42526; -.
DR PRO; PR:P42526; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; TAS:dictyBase.
DR GO; GO:0051015; F:actin filament binding; IDA:dictyBase.
DR GO; GO:0005504; F:fatty acid binding; IDA:dictyBase.
DR GO; GO:0008289; F:lipid binding; IDA:dictyBase.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IEA:InterPro.
DR GO; GO:0010447; P:response to acidic pH; IMP:dictyBase.
DR CDD; cd00257; Fascin; 1.
DR InterPro; IPR008999; Actin-crosslinking.
DR InterPro; IPR022768; Fascin-domain.
DR Pfam; PF06268; Fascin; 1.
DR SUPFAM; SSF50405; SSF50405; 1.
PE 1: Evidence at protein level;
KW Actin-binding; Cell membrane; Cytoplasm; Direct protein sequencing;
KW Disulfide bond; Lipoprotein; Membrane; Myristate; Phosphoprotein;
KW Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..118
FT /note="Hisactophilin-2"
FT /id="PRO_0000083905"
FT REPEAT 34..46
FT /note="1"
FT REPEAT 74..86
FT /note="2"
FT REGION 8..109
FT /note="Contains several HHXH repeats"
FT REGION 34..86
FT /note="2 X 13 AA approximate repeats"
FT REGION 99..118
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000269|PubMed:7822284"
SQ SEQUENCE 118 AA; 13634 MW; 992D50597F8CC413 CRC64;
MGNRAFKAHN GHYLSAEHDH VKTHHGHHDH HTHFHIENHG SKVALRTHCG KYVSIGDHKQ
VYLSHHLHGD HSLFHLEHHH GKVSIKGHHH HYISVDGHGH VSTSHHHDHH ATFEEHIL
