HAUS1_MOUSE
ID HAUS1_MOUSE Reviewed; 278 AA.
AC Q8BHX1; Q3UIH2; Q8R1M1;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=HAUS augmin-like complex subunit 1;
DE AltName: Full=Coiled-coil domain-containing protein 5;
GN Name=Haus1; Synonyms=Ccdc5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Kidney, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Contributes to mitotic spindle assembly, maintenance of
CC centrosome integrity and completion of cytokinesis as part of the HAUS
CC augmin-like complex. {ECO:0000250}.
CC -!- SUBUNIT: Component of the HAUS augmin-like complex. The complex
CC interacts with the gamma-tubulin ring complex and this interaction is
CC required for spindle assembly. Associates with microtubules. The
CC interaction with microtubules is strong during mitosis, while it is
CC weak or absent during interphase. It is unclear whether this
CC interaction is direct or indirect (By similarity). Interacts with EML3
CC (phosphorylated at 'Thr-882') (By similarity).
CC {ECO:0000250|UniProtKB:Q96CS2}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q96CS2}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC {ECO:0000250|UniProtKB:Q96CS2}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q96CS2}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000250|UniProtKB:Q96CS2}. Note=Localizes with the spindle poles
CC in mitotic cells. In interphase, localized at the centrosome and
CC diffusely in the cytoplasm. Localizes to mitotic spindle microtubules
CC (By similarity). {ECO:0000250|UniProtKB:Q96CS2}.
CC -!- SIMILARITY: Belongs to the HAUS1 family. {ECO:0000305}.
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DR EMBL; AK076912; BAC36526.1; -; mRNA.
DR EMBL; AK146923; BAE27534.1; -; mRNA.
DR EMBL; BC024400; AAH24400.1; -; mRNA.
DR CCDS; CCDS29357.1; -.
DR RefSeq; NP_666201.1; NM_146089.2.
DR AlphaFoldDB; Q8BHX1; -.
DR SMR; Q8BHX1; -.
DR BioGRID; 230424; 43.
DR IntAct; Q8BHX1; 42.
DR MINT; Q8BHX1; -.
DR STRING; 10090.ENSMUSP00000035826; -.
DR iPTMnet; Q8BHX1; -.
DR PhosphoSitePlus; Q8BHX1; -.
DR EPD; Q8BHX1; -.
DR MaxQB; Q8BHX1; -.
DR PaxDb; Q8BHX1; -.
DR PeptideAtlas; Q8BHX1; -.
DR PRIDE; Q8BHX1; -.
DR ProteomicsDB; 270937; -.
DR Antibodypedia; 22454; 103 antibodies from 18 providers.
DR DNASU; 225745; -.
DR Ensembl; ENSMUST00000048192; ENSMUSP00000035826; ENSMUSG00000041840.
DR GeneID; 225745; -.
DR KEGG; mmu:225745; -.
DR UCSC; uc008frs.1; mouse.
DR CTD; 115106; -.
DR MGI; MGI:2385076; Haus1.
DR VEuPathDB; HostDB:ENSMUSG00000041840; -.
DR eggNOG; ENOG502QSQA; Eukaryota.
DR GeneTree; ENSGT00390000006029; -.
DR HOGENOM; CLU_063322_0_0_1; -.
DR InParanoid; Q8BHX1; -.
DR OrthoDB; 1350537at2759; -.
DR PhylomeDB; Q8BHX1; -.
DR TreeFam; TF331717; -.
DR Reactome; R-MMU-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-MMU-380259; Loss of Nlp from mitotic centrosomes.
DR Reactome; R-MMU-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-MMU-380284; Loss of proteins required for interphase microtubule organization from the centrosome.
DR Reactome; R-MMU-380320; Recruitment of NuMA to mitotic centrosomes.
DR Reactome; R-MMU-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-MMU-8854518; AURKA Activation by TPX2.
DR BioGRID-ORCS; 225745; 25 hits in 71 CRISPR screens.
DR ChiTaRS; Haus1; mouse.
DR PRO; PR:Q8BHX1; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q8BHX1; protein.
DR Bgee; ENSMUSG00000041840; Expressed in spermatid and 77 other tissues.
DR ExpressionAtlas; Q8BHX1; baseline and differential.
DR Genevisible; Q8BHX1; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0070652; C:HAUS complex; ISS:UniProtKB.
DR GO; GO:1990498; C:mitotic spindle microtubule; ISS:UniProtKB.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007098; P:centrosome cycle; ISS:UniProtKB.
DR GO; GO:0051225; P:spindle assembly; ISS:UniProtKB.
DR InterPro; IPR026243; HAUS1.
DR PANTHER; PTHR31570; PTHR31570; 1.
DR PRINTS; PR02087; HAUSAUGMINL1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Reference proteome.
FT CHAIN 1..278
FT /note="HAUS augmin-like complex subunit 1"
FT /id="PRO_0000089396"
FT COILED 49..79
FT /evidence="ECO:0000255"
FT COILED 128..178
FT /evidence="ECO:0000255"
FT COILED 249..277
FT /evidence="ECO:0000255"
FT CONFLICT 159
FT /note="E -> K (in Ref. 1; BAC36526)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 278 AA; 31379 MW; EE77CE588774E92A CRC64;
MAAPEEKALQ VAEWLKKVFG DHPIPQYEMN SRTTEILYHL SERNRVRDRD ISLVIEDLKQ
KASEYESEAK RLEDFLMESV NFSPANLSKS GSRFLNALVD SAIALEIKDT SLASFIPAVN
DLTSDLFRTK SKSEEMKLEL GKLEKNLTAT LVLEKCLRED LKKAELQLSA EKAKVDSRLQ
NMDFLKAKAA EFRFGIKAAE EQLSARGMDA SLSHRSLAAL SEKLSELKEQ TIPLKKKLES
YLDLMPSPSL AQLKIEEAKR ELDAIEAELT KKVDMMGL