HAUS2_HUMAN
ID HAUS2_HUMAN Reviewed; 235 AA.
AC Q9NVX0; C9JH36; Q9H9B3;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=HAUS augmin-like complex subunit 2;
DE AltName: Full=Centrosomal protein of 27 kDa;
DE Short=Cep27;
GN Name=HAUS2; Synonyms=C15orf25, CEP27;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human chromosome
RT 15.";
RL Nature 440:671-675(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Lymphoblast;
RX PubMed=14654843; DOI=10.1038/nature02166;
RA Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M.;
RT "Proteomic characterization of the human centrosome by protein correlation
RT profiling.";
RL Nature 426:570-574(2003).
RN [5]
RP IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, FUNCTION, AND SUBCELLULAR
RP LOCATION.
RX PubMed=19427217; DOI=10.1016/j.cub.2009.04.033;
RA Lawo S., Bashkurov M., Mullin M., Ferreria M.G., Kittler R., Habermann B.,
RA Tagliaferro A., Poser I., Hutchins J.R.A., Hegemann B., Pinchev D.,
RA Buchholz F., Peters J.-M., Hyman A.A., Gingras A.-C., Pelletier L.;
RT "HAUS, the 8-subunit human augmin complex, regulates centrosome and spindle
RT integrity.";
RL Curr. Biol. 19:816-826(2009).
RN [6]
RP IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, FUNCTION, AND SUBCELLULAR
RP LOCATION.
RX PubMed=19369198; DOI=10.1073/pnas.0901587106;
RA Uehara R., Nozawa R.-S., Tomioka A., Petry S., Vale R.D., Obuse C.,
RA Goshima G.;
RT "The augmin complex plays a critical role in spindle microtubule generation
RT for mitotic progression and cytokinesis in human cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:6998-7003(2009).
RN [7]
RP INTERACTION WITH EML3, AND SUBCELLULAR LOCATION.
RX PubMed=30723163; DOI=10.1074/jbc.ra118.007164;
RA Luo J., Yang B., Xin G., Sun M., Zhang B., Guo X., Jiang Q., Zhang C.;
RT "The microtubule-associated protein EML3 regulates mitotic spindle assembly
RT by recruiting the Augmin complex to spindle microtubules.";
RL J. Biol. Chem. 294:5643-5656(2019).
CC -!- FUNCTION: Contributes to mitotic spindle assembly, maintenance of
CC centrosome integrity and completion of cytokinesis as part of the HAUS
CC augmin-like complex. {ECO:0000269|PubMed:19369198,
CC ECO:0000269|PubMed:19427217}.
CC -!- SUBUNIT: Component of the HAUS augmin-like complex. The complex
CC interacts with the gamma-tubulin ring complex and this interaction is
CC required for spindle assembly. Interacts with EML3 (phosphorylated at
CC 'Thr-881') (PubMed:30723163). {ECO:0000269|PubMed:19369198,
CC ECO:0000269|PubMed:19427217, ECO:0000269|PubMed:30723163}.
CC -!- INTERACTION:
CC Q9NVX0; Q7Z4H7: HAUS6; NbExp=10; IntAct=EBI-720080, EBI-2558196;
CC Q9NVX0; Q13426: XRCC4; NbExp=3; IntAct=EBI-720080, EBI-717592;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:14654843,
CC ECO:0000269|PubMed:19369198, ECO:0000269|PubMed:19427217}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:19369198,
CC ECO:0000269|PubMed:19427217, ECO:0000269|PubMed:30723163}.
CC Note=Localizes to interphase centrosomes and to mitotic spindle
CC microtubules. {ECO:0000269|PubMed:19369198,
CC ECO:0000269|PubMed:19427217, ECO:0000269|PubMed:30723163}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9NVX0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NVX0-2; Sequence=VSP_012223;
CC Name=3;
CC IsoId=Q9NVX0-3; Sequence=VSP_040918;
CC -!- SIMILARITY: Belongs to the HAUS2 family. {ECO:0000305}.
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DR EMBL; AK001322; BAA91623.1; -; mRNA.
DR EMBL; AK022939; BAB14319.1; -; mRNA.
DR EMBL; AC018362; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC010903; AAH10903.1; -; mRNA.
DR CCDS; CCDS10090.1; -. [Q9NVX0-1]
DR CCDS; CCDS45247.1; -. [Q9NVX0-3]
DR RefSeq; NP_001123919.1; NM_001130447.1. [Q9NVX0-3]
DR RefSeq; NP_001310560.1; NM_001323631.1. [Q9NVX0-2]
DR RefSeq; NP_001310561.1; NM_001323632.1. [Q9NVX0-2]
DR RefSeq; NP_060567.1; NM_018097.2. [Q9NVX0-1]
DR RefSeq; XP_016877885.1; XM_017022396.1. [Q9NVX0-2]
DR RefSeq; XP_016877886.1; XM_017022397.1. [Q9NVX0-2]
DR AlphaFoldDB; Q9NVX0; -.
DR SMR; Q9NVX0; -.
DR BioGRID; 120445; 130.
DR ComplexPortal; CPX-1847; HAUS complex.
DR CORUM; Q9NVX0; -.
DR DIP; DIP-48836N; -.
DR IntAct; Q9NVX0; 49.
DR MINT; Q9NVX0; -.
DR STRING; 9606.ENSP00000260372; -.
DR iPTMnet; Q9NVX0; -.
DR PhosphoSitePlus; Q9NVX0; -.
DR BioMuta; HAUS2; -.
DR DMDM; 56748883; -.
DR EPD; Q9NVX0; -.
DR jPOST; Q9NVX0; -.
DR MassIVE; Q9NVX0; -.
DR MaxQB; Q9NVX0; -.
DR PaxDb; Q9NVX0; -.
DR PeptideAtlas; Q9NVX0; -.
DR PRIDE; Q9NVX0; -.
DR ProteomicsDB; 82873; -. [Q9NVX0-1]
DR ProteomicsDB; 82874; -. [Q9NVX0-2]
DR ProteomicsDB; 82875; -. [Q9NVX0-3]
DR Antibodypedia; 23697; 67 antibodies from 19 providers.
DR DNASU; 55142; -.
DR Ensembl; ENST00000260372.8; ENSP00000260372.3; ENSG00000137814.12. [Q9NVX0-1]
DR Ensembl; ENST00000568876.5; ENSP00000456578.1; ENSG00000137814.12. [Q9NVX0-3]
DR GeneID; 55142; -.
DR KEGG; hsa:55142; -.
DR MANE-Select; ENST00000260372.8; ENSP00000260372.3; NM_018097.3; NP_060567.1.
DR UCSC; uc001zqe.4; human. [Q9NVX0-1]
DR CTD; 55142; -.
DR GeneCards; HAUS2; -.
DR HGNC; HGNC:25530; HAUS2.
DR HPA; ENSG00000137814; Tissue enhanced (bone).
DR MIM; 613429; gene.
DR neXtProt; NX_Q9NVX0; -.
DR OpenTargets; ENSG00000137814; -.
DR PharmGKB; PA165479051; -.
DR VEuPathDB; HostDB:ENSG00000137814; -.
DR eggNOG; ENOG502RY4E; Eukaryota.
DR GeneTree; ENSGT00390000004927; -.
DR InParanoid; Q9NVX0; -.
DR OMA; HQWDLSP; -.
DR PhylomeDB; Q9NVX0; -.
DR TreeFam; TF333194; -.
DR PathwayCommons; Q9NVX0; -.
DR Reactome; R-HSA-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-HSA-380259; Loss of Nlp from mitotic centrosomes.
DR Reactome; R-HSA-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-HSA-380284; Loss of proteins required for interphase microtubule organization from the centrosome.
DR Reactome; R-HSA-380320; Recruitment of NuMA to mitotic centrosomes.
DR Reactome; R-HSA-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-HSA-8854518; AURKA Activation by TPX2.
DR SignaLink; Q9NVX0; -.
DR SIGNOR; Q9NVX0; -.
DR BioGRID-ORCS; 55142; 283 hits in 1081 CRISPR screens.
DR ChiTaRS; HAUS2; human.
DR GenomeRNAi; 55142; -.
DR Pharos; Q9NVX0; Tbio.
DR PRO; PR:Q9NVX0; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q9NVX0; protein.
DR Bgee; ENSG00000137814; Expressed in renal medulla and 214 other tissues.
DR ExpressionAtlas; Q9NVX0; baseline and differential.
DR Genevisible; Q9NVX0; HS.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0070652; C:HAUS complex; IDA:UniProtKB.
DR GO; GO:1990498; C:mitotic spindle microtubule; IDA:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007098; P:centrosome cycle; IMP:UniProtKB.
DR GO; GO:0007020; P:microtubule nucleation; IBA:GO_Central.
DR GO; GO:0051225; P:spindle assembly; IMP:UniProtKB.
DR InterPro; IPR028346; HAUS2.
DR InterPro; IPR026242; HAUS2_metazoa.
DR PANTHER; PTHR16039; PTHR16039; 1.
DR Pfam; PF15003; HAUS2; 1.
DR PRINTS; PR02088; HAUSAUGMINL2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Coiled coil; Cytoplasm;
KW Cytoskeleton; Microtubule; Mitosis; Reference proteome.
FT CHAIN 1..235
FT /note="HAUS augmin-like complex subunit 2"
FT /id="PRO_0000089484"
FT COILED 51..115
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..94
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_012223"
FT VAR_SEQ 32..62
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_040918"
FT VARIANT 76
FT /note="T -> P (in dbSNP:rs34678957)"
FT /id="VAR_050793"
SQ SEQUENCE 235 AA; 26933 MW; E1475D00313FCA7F CRC64;
MAAANPWDPA SAPNGAGLVL GHFIASGMVN QEMLNMSKKT VSCFVNFTRL QQITNIQAEI
YQKNLEIELL KLEKDTADVV HPFFLAQKCH TLQSMNNHLE AVLKEKRSLR QRLLKPMCQE
NLPIEAVYHR YMVHLLELAV TFIERLETHL ETIRNIPHLA ANLKKMNQAL AKMDILVTET
EELAENILKW RKQQNEVSSC IPKILAEESY LYKHDIIMPP LPFTSKVHVQ TINAK
