HAUS3_HUMAN
ID HAUS3_HUMAN Reviewed; 603 AA.
AC Q68CZ6; B4DF64; O43606; Q8TAZ5; Q9BTJ9;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=HAUS augmin-like complex subunit 3;
GN Name=HAUS3; Synonyms=C4orf15;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Pribill I., Barnes G.T., Chen J., Church D., Buckler A., Baxendale S.,
RA Bates G.P., Lehrach H., Gusella M.J., Duyao M.P., Ambrose C.M.,
RA MacDonald M.E., Gusella J.F.;
RT "Comparison of exon trapping and sequence-based methods of gene finding.";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Endometrial adenocarcinoma;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lung carcinoma, and Rhabdomyosarcoma;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Lymphoblast;
RX PubMed=14654843; DOI=10.1038/nature02166;
RA Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M.;
RT "Proteomic characterization of the human centrosome by protein correlation
RT profiling.";
RL Nature 426:570-574(2003).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [9]
RP IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, AND FUNCTION.
RX PubMed=19427217; DOI=10.1016/j.cub.2009.04.033;
RA Lawo S., Bashkurov M., Mullin M., Ferreria M.G., Kittler R., Habermann B.,
RA Tagliaferro A., Poser I., Hutchins J.R.A., Hegemann B., Pinchev D.,
RA Buchholz F., Peters J.-M., Hyman A.A., Gingras A.-C., Pelletier L.;
RT "HAUS, the 8-subunit human augmin complex, regulates centrosome and spindle
RT integrity.";
RL Curr. Biol. 19:816-826(2009).
RN [10]
RP IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, FUNCTION, AND SUBCELLULAR
RP LOCATION.
RX PubMed=19369198; DOI=10.1073/pnas.0901587106;
RA Uehara R., Nozawa R.-S., Tomioka A., Petry S., Vale R.D., Obuse C.,
RA Goshima G.;
RT "The augmin complex plays a critical role in spindle microtubule generation
RT for mitotic progression and cytokinesis in human cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:6998-7003(2009).
RN [11]
RP INTERACTION WITH EML3, AND SUBCELLULAR LOCATION.
RX PubMed=30723163; DOI=10.1074/jbc.ra118.007164;
RA Luo J., Yang B., Xin G., Sun M., Zhang B., Guo X., Jiang Q., Zhang C.;
RT "The microtubule-associated protein EML3 regulates mitotic spindle assembly
RT by recruiting the Augmin complex to spindle microtubules.";
RL J. Biol. Chem. 294:5643-5656(2019).
CC -!- FUNCTION: Contributes to mitotic spindle assembly, maintenance of
CC centrosome integrity and completion of cytokinesis as part of the HAUS
CC augmin-like complex. {ECO:0000269|PubMed:19369198,
CC ECO:0000269|PubMed:19427217}.
CC -!- SUBUNIT: Component of the HAUS augmin-like complex. The complex
CC interacts with the gamma-tubulin ring complex and this interaction is
CC required for spindle assembly. Interacts with EML3 (phosphorylated at
CC 'Thr-881') (PubMed:30723163). {ECO:0000269|PubMed:19369198,
CC ECO:0000269|PubMed:19427217, ECO:0000269|PubMed:30723163}.
CC -!- INTERACTION:
CC Q68CZ6; X5D778: ANKRD11; NbExp=3; IntAct=EBI-2558217, EBI-17183751;
CC Q68CZ6; Q0VDD7: BRME1; NbExp=3; IntAct=EBI-2558217, EBI-741210;
CC Q68CZ6; O76071: CIAO1; NbExp=3; IntAct=EBI-2558217, EBI-725145;
CC Q68CZ6; Q9P2W3: GNG13; NbExp=3; IntAct=EBI-2558217, EBI-11427343;
CC Q68CZ6; Q7Z4H7: HAUS6; NbExp=4; IntAct=EBI-2558217, EBI-2558196;
CC Q68CZ6; O14901: KLF11; NbExp=3; IntAct=EBI-2558217, EBI-948266;
CC Q68CZ6; Q9P2K3-2: RCOR3; NbExp=3; IntAct=EBI-2558217, EBI-1504830;
CC Q68CZ6; P40222: TXLNA; NbExp=9; IntAct=EBI-2558217, EBI-359793;
CC Q68CZ6; Q8N3L3: TXLNB; NbExp=3; IntAct=EBI-2558217, EBI-6116822;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:14654843,
CC ECO:0000269|PubMed:19427217}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:19427217, ECO:0000269|PubMed:30723163}.
CC Note=Localizes to interphase centrosomes and to mitotic spindle
CC microtubules. {ECO:0000269|PubMed:19427217,
CC ECO:0000269|PubMed:30723163}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q68CZ6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q68CZ6-2; Sequence=VSP_057022, VSP_057023;
CC -!- SIMILARITY: Belongs to the HAUS3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB97010.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH03648.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH03648.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAH25356.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF040964; AAB97010.1; ALT_INIT; mRNA.
DR EMBL; AK293948; BAG57325.1; -; mRNA.
DR EMBL; CR749640; CAH18434.1; -; mRNA.
DR EMBL; AL158068; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471131; EAW82537.1; -; Genomic_DNA.
DR EMBL; BC003648; AAH03648.1; ALT_FRAME; mRNA.
DR EMBL; BC025356; AAH25356.1; ALT_INIT; mRNA.
DR CCDS; CCDS33941.1; -. [Q68CZ6-1]
DR RefSeq; NP_001290072.1; NM_001303143.1. [Q68CZ6-1]
DR RefSeq; NP_078787.2; NM_024511.6. [Q68CZ6-1]
DR PDB; 6PTB; X-ray; 2.15 A; C/F=154-162.
DR PDB; 6PTE; X-ray; 1.90 A; C/D/G/J=154-162.
DR PDBsum; 6PTB; -.
DR PDBsum; 6PTE; -.
DR AlphaFoldDB; Q68CZ6; -.
DR SMR; Q68CZ6; -.
DR BioGRID; 122667; 111.
DR ComplexPortal; CPX-1847; HAUS complex.
DR CORUM; Q68CZ6; -.
DR DIP; DIP-48832N; -.
DR IntAct; Q68CZ6; 68.
DR MINT; Q68CZ6; -.
DR STRING; 9606.ENSP00000243706; -.
DR iPTMnet; Q68CZ6; -.
DR PhosphoSitePlus; Q68CZ6; -.
DR BioMuta; HAUS3; -.
DR DMDM; 74708788; -.
DR EPD; Q68CZ6; -.
DR jPOST; Q68CZ6; -.
DR MassIVE; Q68CZ6; -.
DR MaxQB; Q68CZ6; -.
DR PaxDb; Q68CZ6; -.
DR PeptideAtlas; Q68CZ6; -.
DR PRIDE; Q68CZ6; -.
DR ProteomicsDB; 4011; -.
DR ProteomicsDB; 66040; -. [Q68CZ6-1]
DR Antibodypedia; 43178; 134 antibodies from 23 providers.
DR DNASU; 79441; -.
DR Ensembl; ENST00000243706.8; ENSP00000243706.4; ENSG00000214367.9. [Q68CZ6-1]
DR Ensembl; ENST00000443786.3; ENSP00000392903.2; ENSG00000214367.9. [Q68CZ6-1]
DR Ensembl; ENST00000506763.5; ENSP00000427350.1; ENSG00000214367.9. [Q68CZ6-2]
DR Ensembl; ENST00000672739.1; ENSP00000500512.1; ENSG00000214367.9. [Q68CZ6-1]
DR GeneID; 79441; -.
DR KEGG; hsa:79441; -.
DR MANE-Select; ENST00000443786.3; ENSP00000392903.2; NM_001303143.2; NP_001290072.1.
DR UCSC; uc003ges.2; human. [Q68CZ6-1]
DR CTD; 79441; -.
DR DisGeNET; 79441; -.
DR GeneCards; HAUS3; -.
DR HGNC; HGNC:28719; HAUS3.
DR HPA; ENSG00000214367; Low tissue specificity.
DR MIM; 613430; gene.
DR neXtProt; NX_Q68CZ6; -.
DR OpenTargets; ENSG00000214367; -.
DR PharmGKB; PA165664132; -.
DR VEuPathDB; HostDB:ENSG00000214367; -.
DR eggNOG; ENOG502R4I5; Eukaryota.
DR GeneTree; ENSGT00390000011904; -.
DR HOGENOM; CLU_031795_0_0_1; -.
DR InParanoid; Q68CZ6; -.
DR OMA; SFLEWFC; -.
DR OrthoDB; 380555at2759; -.
DR PhylomeDB; Q68CZ6; -.
DR TreeFam; TF331151; -.
DR PathwayCommons; Q68CZ6; -.
DR Reactome; R-HSA-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-HSA-380259; Loss of Nlp from mitotic centrosomes.
DR Reactome; R-HSA-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-HSA-380284; Loss of proteins required for interphase microtubule organization from the centrosome.
DR Reactome; R-HSA-380320; Recruitment of NuMA to mitotic centrosomes.
DR Reactome; R-HSA-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-HSA-8854518; AURKA Activation by TPX2.
DR SignaLink; Q68CZ6; -.
DR SIGNOR; Q68CZ6; -.
DR BioGRID-ORCS; 79441; 751 hits in 1089 CRISPR screens.
DR GeneWiki; HAUS3; -.
DR GenomeRNAi; 79441; -.
DR Pharos; Q68CZ6; Tbio.
DR PRO; PR:Q68CZ6; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q68CZ6; protein.
DR Bgee; ENSG00000214367; Expressed in secondary oocyte and 178 other tissues.
DR ExpressionAtlas; Q68CZ6; baseline and differential.
DR Genevisible; Q68CZ6; HS.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0070652; C:HAUS complex; IDA:UniProtKB.
DR GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR GO; GO:1990498; C:mitotic spindle microtubule; IDA:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007098; P:centrosome cycle; IMP:UniProtKB.
DR GO; GO:0051225; P:spindle assembly; IMP:UniProtKB.
DR InterPro; IPR026206; HAUS3.
DR InterPro; IPR032733; HAUS3_N.
DR PANTHER; PTHR19378; PTHR19378; 1.
DR Pfam; PF14932; HAUS-augmin3; 1.
DR PRINTS; PR02089; HAUSAUGMINL3.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Cell cycle; Cell division;
KW Coiled coil; Cytoplasm; Cytoskeleton; Microtubule; Mitosis;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:19413330"
FT CHAIN 2..603
FT /note="HAUS augmin-like complex subunit 3"
FT /id="PRO_0000301951"
FT COILED 93..177
FT /evidence="ECO:0000255"
FT COILED 305..336
FT /evidence="ECO:0000255"
FT COILED 389..426
FT /evidence="ECO:0000255"
FT COILED 458..495
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:19413330"
FT VAR_SEQ 477..489
FT /note="KLKQNISLVQDQL -> AGEWHEPRRRGLQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_057022"
FT VAR_SEQ 490..603
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_057023"
FT VARIANT 586
FT /note="I -> T (in dbSNP:rs11937432)"
FT /id="VAR_034912"
SQ SEQUENCE 603 AA; 69650 MW; C65B80CC58F326E9 CRC64;
MSCGNEFVET LKKIGYPKAD NLNGEDFDWL FEGVEDESFL KWFCGNVNEQ NVLSERELEA
FSILQKSGKP ILEGAALDEA LKTCKTSDLK TPRLDDKELE KLEDEVQTLL KLKNLKIQRR
NKCQLMASVT SHKSLRLNAK EEEATKKLKQ SQGILNAMIT KISNELQALT DEVTQLMMFF
RHSNLGQGTN PLVFLSQFSL EKYLSQEEQS TAALTLYTKK QFFQGIHEVV ESSNEDNFQL
LDIQTPSICD NQEILEERRL EMARLQLAYI CAQHQLIHLK ASNSSMKSSI KWAEESLHSL
TSKAVDKENL DAKISSLTSE IMKLEKEVTQ IKDRSLPAVV RENAQLLNMP VVKGDFDLQI
AKQDYYTARQ ELVLNQLIKQ KASFELLQLS YEIELRKHRD IYRQLENLVQ ELSQSNMMLY
KQLEMLTDPS VSQQINPRNT IDTKDYSTHR LYQVLEGENK KKELFLTHGN LEEVAEKLKQ
NISLVQDQLA VSAQEHSFFL SKRNKDVDML CDTLYQGGNQ LLLSDQELTE QFHKVESQLN
KLNHLLTDIL ADVKTKRKTL ANNKLHQMER EFYVYFLKDE DYLKDIVENL ETQSKIKAVS
LED
