HAUS4_HUMAN
ID HAUS4_HUMAN Reviewed; 363 AA.
AC Q9H6D7; B7WP17; D3DS34; Q86T15; Q86T16; Q86U43; Q9NX59;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=HAUS augmin-like complex subunit 4;
GN Name=HAUS4; Synonyms=C14orf94;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Kidney epithelium, and Signet-ring cell carcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
RC TISSUE=Fetal brain, and T-cell;
RA Li W.B., Gruber C., Jessee J., Polayes D.;
RT "Full-length cDNA libraries and normalization.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12508121; DOI=10.1038/nature01348;
RA Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C.,
RA Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A.,
RA Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H.,
RA Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T.,
RA Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B.,
RA Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D.,
RA Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R.,
RA Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S.,
RA Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C.,
RA Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S.,
RA Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C.,
RA Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P.,
RA Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M.,
RA Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V.,
RA Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J.,
RA Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F.,
RA Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F.,
RA Waterston R., Hood L., Weissenbach J.;
RT "The DNA sequence and analysis of human chromosome 14.";
RL Nature 421:601-607(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Choriocarcinoma, and Melanoma;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Lymphoblast;
RX PubMed=14654843; DOI=10.1038/nature02166;
RA Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M.;
RT "Proteomic characterization of the human centrosome by protein correlation
RT profiling.";
RL Nature 426:570-574(2003).
RN [7]
RP IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, FUNCTION, AND SUBCELLULAR
RP LOCATION.
RX PubMed=19427217; DOI=10.1016/j.cub.2009.04.033;
RA Lawo S., Bashkurov M., Mullin M., Ferreria M.G., Kittler R., Habermann B.,
RA Tagliaferro A., Poser I., Hutchins J.R.A., Hegemann B., Pinchev D.,
RA Buchholz F., Peters J.-M., Hyman A.A., Gingras A.-C., Pelletier L.;
RT "HAUS, the 8-subunit human augmin complex, regulates centrosome and spindle
RT integrity.";
RL Curr. Biol. 19:816-826(2009).
RN [8]
RP IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, FUNCTION, AND SUBCELLULAR
RP LOCATION.
RX PubMed=19369198; DOI=10.1073/pnas.0901587106;
RA Uehara R., Nozawa R.-S., Tomioka A., Petry S., Vale R.D., Obuse C.,
RA Goshima G.;
RT "The augmin complex plays a critical role in spindle microtubule generation
RT for mitotic progression and cytokinesis in human cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:6998-7003(2009).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [10]
RP INTERACTION WITH EML3, AND SUBCELLULAR LOCATION.
RX PubMed=30723163; DOI=10.1074/jbc.ra118.007164;
RA Luo J., Yang B., Xin G., Sun M., Zhang B., Guo X., Jiang Q., Zhang C.;
RT "The microtubule-associated protein EML3 regulates mitotic spindle assembly
RT by recruiting the Augmin complex to spindle microtubules.";
RL J. Biol. Chem. 294:5643-5656(2019).
CC -!- FUNCTION: Contributes to mitotic spindle assembly, maintenance of
CC centrosome integrity and completion of cytokinesis as part of the HAUS
CC augmin-like complex. {ECO:0000269|PubMed:19369198,
CC ECO:0000269|PubMed:19427217}.
CC -!- SUBUNIT: Component of the HAUS augmin-like complex. The complex
CC interacts with the gamma-tubulin ring complex and this interaction is
CC required for spindle assembly. Interacts with EML3 (phosphorylated at
CC 'Thr-881') (PubMed:30723163). {ECO:0000269|PubMed:19369198,
CC ECO:0000269|PubMed:19427217, ECO:0000269|PubMed:30723163}.
CC -!- INTERACTION:
CC Q9H6D7; Q96CS2: HAUS1; NbExp=11; IntAct=EBI-2558168, EBI-2514791;
CC Q9H6D7; Q7Z4H7: HAUS6; NbExp=3; IntAct=EBI-2558168, EBI-2558196;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:14654843,
CC ECO:0000269|PubMed:19369198, ECO:0000269|PubMed:19427217}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:19369198,
CC ECO:0000269|PubMed:19427217, ECO:0000269|PubMed:30723163}.
CC Note=Localizes to interphase centrosomes and to mitotic spindle
CC microtubules. {ECO:0000269|PubMed:19369198,
CC ECO:0000269|PubMed:19427217, ECO:0000269|PubMed:30723163}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q9H6D7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9H6D7-2; Sequence=VSP_007853;
CC Name=3;
CC IsoId=Q9H6D7-3; Sequence=VSP_007851;
CC Name=4;
CC IsoId=Q9H6D7-4; Sequence=VSP_007852;
CC -!- SIMILARITY: Belongs to the HAUS4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAD62584.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK026016; BAB15322.1; -; mRNA.
DR EMBL; AK000431; BAA91161.1; -; mRNA.
DR EMBL; BX247981; CAD62315.1; -; mRNA.
DR EMBL; BX247995; CAD62328.1; -; mRNA.
DR EMBL; BX248256; CAD62584.1; ALT_INIT; mRNA.
DR EMBL; AL132780; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471078; EAW66207.1; -; Genomic_DNA.
DR EMBL; CH471078; EAW66206.1; -; Genomic_DNA.
DR EMBL; CH471078; EAW66209.1; -; Genomic_DNA.
DR EMBL; CH471078; EAW66210.1; -; Genomic_DNA.
DR EMBL; CH471078; EAW66213.1; -; Genomic_DNA.
DR EMBL; BC001916; AAH01916.1; -; mRNA.
DR EMBL; BC002554; AAH02554.1; -; mRNA.
DR CCDS; CCDS53886.1; -. [Q9H6D7-4]
DR CCDS; CCDS9580.1; -. [Q9H6D7-1]
DR RefSeq; NP_001159741.1; NM_001166269.1. [Q9H6D7-1]
DR RefSeq; NP_001159742.1; NM_001166270.1. [Q9H6D7-4]
DR RefSeq; NP_060285.2; NM_017815.2. [Q9H6D7-1]
DR AlphaFoldDB; Q9H6D7; -.
DR SMR; Q9H6D7; -.
DR BioGRID; 120270; 108.
DR ComplexPortal; CPX-1847; HAUS complex.
DR DIP; DIP-48837N; -.
DR IntAct; Q9H6D7; 50.
DR MINT; Q9H6D7; -.
DR STRING; 9606.ENSP00000206474; -.
DR iPTMnet; Q9H6D7; -.
DR PhosphoSitePlus; Q9H6D7; -.
DR BioMuta; HAUS4; -.
DR DMDM; 33301114; -.
DR EPD; Q9H6D7; -.
DR jPOST; Q9H6D7; -.
DR MassIVE; Q9H6D7; -.
DR MaxQB; Q9H6D7; -.
DR PaxDb; Q9H6D7; -.
DR PeptideAtlas; Q9H6D7; -.
DR PRIDE; Q9H6D7; -.
DR ProteomicsDB; 80977; -. [Q9H6D7-1]
DR ProteomicsDB; 80978; -. [Q9H6D7-2]
DR ProteomicsDB; 80979; -. [Q9H6D7-3]
DR ProteomicsDB; 80980; -. [Q9H6D7-4]
DR Antibodypedia; 22337; 126 antibodies from 21 providers.
DR DNASU; 54930; -.
DR Ensembl; ENST00000206474.11; ENSP00000206474.7; ENSG00000092036.19. [Q9H6D7-1]
DR Ensembl; ENST00000342454.12; ENSP00000342776.8; ENSG00000092036.19. [Q9H6D7-4]
DR Ensembl; ENST00000347758.6; ENSP00000343473.2; ENSG00000092036.19. [Q9H6D7-2]
DR Ensembl; ENST00000490506.5; ENSP00000452260.1; ENSG00000092036.19. [Q9H6D7-3]
DR Ensembl; ENST00000541587.6; ENSP00000441026.1; ENSG00000092036.19. [Q9H6D7-1]
DR Ensembl; ENST00000555367.5; ENSP00000452239.1; ENSG00000092036.19. [Q9H6D7-4]
DR Ensembl; ENST00000555986.5; ENSP00000451018.1; ENSG00000092036.19. [Q9H6D7-4]
DR GeneID; 54930; -.
DR KEGG; hsa:54930; -.
DR MANE-Select; ENST00000541587.6; ENSP00000441026.1; NM_001166269.2; NP_001159741.1.
DR UCSC; uc001whq.4; human. [Q9H6D7-1]
DR CTD; 54930; -.
DR DisGeNET; 54930; -.
DR GeneCards; HAUS4; -.
DR HGNC; HGNC:20163; HAUS4.
DR HPA; ENSG00000092036; Low tissue specificity.
DR MIM; 613431; gene.
DR neXtProt; NX_Q9H6D7; -.
DR OpenTargets; ENSG00000092036; -.
DR PharmGKB; PA165478984; -.
DR VEuPathDB; HostDB:ENSG00000092036; -.
DR eggNOG; ENOG502QW4I; Eukaryota.
DR GeneTree; ENSGT00390000014634; -.
DR HOGENOM; CLU_065166_1_0_1; -.
DR InParanoid; Q9H6D7; -.
DR OMA; NWALKEF; -.
DR OrthoDB; 1168055at2759; -.
DR PhylomeDB; Q9H6D7; -.
DR TreeFam; TF330353; -.
DR PathwayCommons; Q9H6D7; -.
DR Reactome; R-HSA-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-HSA-380259; Loss of Nlp from mitotic centrosomes.
DR Reactome; R-HSA-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-HSA-380284; Loss of proteins required for interphase microtubule organization from the centrosome.
DR Reactome; R-HSA-380320; Recruitment of NuMA to mitotic centrosomes.
DR Reactome; R-HSA-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-HSA-8854518; AURKA Activation by TPX2.
DR SignaLink; Q9H6D7; -.
DR SIGNOR; Q9H6D7; -.
DR BioGRID-ORCS; 54930; 729 hits in 1084 CRISPR screens.
DR ChiTaRS; HAUS4; human.
DR GenomeRNAi; 54930; -.
DR Pharos; Q9H6D7; Tbio.
DR PRO; PR:Q9H6D7; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q9H6D7; protein.
DR Bgee; ENSG00000092036; Expressed in blood and 102 other tissues.
DR ExpressionAtlas; Q9H6D7; baseline and differential.
DR Genevisible; Q9H6D7; HS.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0070652; C:HAUS complex; IDA:UniProtKB.
DR GO; GO:1990498; C:mitotic spindle microtubule; IDA:UniProtKB.
DR GO; GO:0051011; F:microtubule minus-end binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007098; P:centrosome cycle; IMP:UniProtKB.
DR GO; GO:0051225; P:spindle assembly; IMP:UniProtKB.
DR InterPro; IPR029327; HAUS4.
DR InterPro; IPR026214; HAUS4_met.
DR PANTHER; PTHR16219; PTHR16219; 1.
DR Pfam; PF14735; HAUS4; 1.
DR PRINTS; PR02090; HAUSAUGMINL4.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Reference proteome.
FT CHAIN 1..363
FT /note="HAUS augmin-like complex subunit 4"
FT /id="PRO_0000089901"
FT VAR_SEQ 1..124
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_007851"
FT VAR_SEQ 111..236
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_007853"
FT VAR_SEQ 111..155
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_007852"
FT CONFLICT 154
FT /note="S -> T (in Ref. 1; BAA91161)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 363 AA; 42400 MW; AAAFA1FFC25189C0 CRC64;
MASGDFCSPG EGMEILQQVC SKQLPPCNLS KEDLLQNPYF SKLLLNLSQH VDESGLSLTL
AKEQAQAWKE VRLHKTTWLR SEILHRVIQE LLVDYYVKIQ DTNVTSEDKK FHETLEQRLL
VTELMRLLGP SQEREIPPLL GLEKADLLEL MPLSEDFVWM RARLQQEVEE QLKKKCFTLL
CYYDPNSDAD SETVKAAKVW KLAEVLVGEQ QQCQDAKSQQ KEQMLLLEKK SAAYSQVLLR
CLTLLQRLLQ EHRLKTQSEL DRINAQYLEV KCGAMILKLR MEELKILSDT YTVEKVEVHR
LIRDRLEGAI HLQEQDMENS RQVLNSYEVL GEEFDRLVKE YTVLKQATEN KRWALQEFSK
VYR
