HAUS5_MOUSE
ID HAUS5_MOUSE Reviewed; 619 AA.
AC Q9D786; Q08EB3; Q0VF86; Q5HZI7; Q6ZQ35; Q8CIK2;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=HAUS augmin-like complex subunit 5;
GN Name=Haus5; Synonyms=Kiaa0841;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 71-619.
RC TISSUE=Embryonic tail;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [5]
RP SEQUENCE REVISION.
RA Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.;
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Contributes to mitotic spindle assembly, maintenance of
CC centrosome integrity and completion of cytokinesis as part of the HAUS
CC augmin-like complex. {ECO:0000250}.
CC -!- SUBUNIT: Component of the HAUS augmin-like complex. The complex
CC interacts with the gamma-tubulin ring complex and this interaction is
CC required for spindle assembly (By similarity). Interacts with EML3
CC (phosphorylated at 'Thr-882') (By similarity).
CC {ECO:0000250|UniProtKB:O94927}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250|UniProtKB:O94927}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000250|UniProtKB:O94927}. Note=Localizes to
CC interphase centrosomes and to mitotic spindle microtubules.
CC {ECO:0000250|UniProtKB:O94927}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9D786-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9D786-2; Sequence=VSP_013929, VSP_013930;
CC -!- SIMILARITY: Belongs to the HAUS5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH23723.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH89002.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK009476; BAB26314.1; -; mRNA.
DR EMBL; CH466593; EDL24000.1; -; Genomic_DNA.
DR EMBL; BC023723; AAH23723.1; ALT_INIT; mRNA.
DR EMBL; BC089002; AAH89002.1; ALT_INIT; mRNA.
DR EMBL; BC118934; AAI18935.1; -; mRNA.
DR EMBL; BC118935; AAI18936.1; -; mRNA.
DR EMBL; AK129228; BAC98038.2; -; Transcribed_RNA.
DR CCDS; CCDS52180.1; -. [Q9D786-1]
DR RefSeq; NP_082275.1; NM_027999.1. [Q9D786-1]
DR AlphaFoldDB; Q9D786; -.
DR SMR; Q9D786; -.
DR BioGRID; 215022; 4.
DR STRING; 10090.ENSMUSP00000019697; -.
DR iPTMnet; Q9D786; -.
DR PhosphoSitePlus; Q9D786; -.
DR EPD; Q9D786; -.
DR MaxQB; Q9D786; -.
DR PaxDb; Q9D786; -.
DR PeptideAtlas; Q9D786; -.
DR PRIDE; Q9D786; -.
DR ProteomicsDB; 270890; -. [Q9D786-1]
DR ProteomicsDB; 270891; -. [Q9D786-2]
DR Antibodypedia; 54102; 93 antibodies from 18 providers.
DR Ensembl; ENSMUST00000019697; ENSMUSP00000019697; ENSMUSG00000078762. [Q9D786-1]
DR Ensembl; ENSMUST00000132862; ENSMUSP00000121739; ENSMUSG00000078762. [Q9D786-2]
DR GeneID; 71909; -.
DR KEGG; mmu:71909; -.
DR UCSC; uc009gfs.2; mouse. [Q9D786-1]
DR CTD; 23354; -.
DR MGI; MGI:1919159; Haus5.
DR VEuPathDB; HostDB:ENSMUSG00000078762; -.
DR eggNOG; ENOG502QVVA; Eukaryota.
DR GeneTree; ENSGT00390000012508; -.
DR HOGENOM; CLU_433414_0_0_1; -.
DR InParanoid; Q9D786; -.
DR OMA; PHEDHFG; -.
DR OrthoDB; 277862at2759; -.
DR PhylomeDB; Q9D786; -.
DR TreeFam; TF333977; -.
DR Reactome; R-MMU-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-MMU-380259; Loss of Nlp from mitotic centrosomes.
DR Reactome; R-MMU-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-MMU-380284; Loss of proteins required for interphase microtubule organization from the centrosome.
DR Reactome; R-MMU-380320; Recruitment of NuMA to mitotic centrosomes.
DR Reactome; R-MMU-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-MMU-8854518; AURKA Activation by TPX2.
DR BioGRID-ORCS; 71909; 25 hits in 72 CRISPR screens.
DR ChiTaRS; Haus5; mouse.
DR PRO; PR:Q9D786; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q9D786; protein.
DR Bgee; ENSMUSG00000078762; Expressed in bone marrow and 67 other tissues.
DR Genevisible; Q9D786; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0070652; C:HAUS complex; ISS:UniProtKB.
DR GO; GO:1990498; C:mitotic spindle microtubule; ISS:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007098; P:centrosome cycle; ISS:UniProtKB.
DR GO; GO:0051225; P:spindle assembly; ISS:UniProtKB.
DR InterPro; IPR029131; HAUS5.
DR InterPro; IPR026215; HAUS5_metazoa.
DR Pfam; PF14817; HAUS5; 1.
DR PRINTS; PR02091; HAUSAUGMINL5.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cell cycle; Cell division; Coiled coil;
KW Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Reference proteome.
FT CHAIN 1..619
FT /note="HAUS augmin-like complex subunit 5"
FT /id="PRO_0000050777"
FT COILED 73..108
FT /evidence="ECO:0000255"
FT COILED 310..395
FT /evidence="ECO:0000255"
FT COILED 550..590
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:O94927"
FT VAR_SEQ 507..517
FT /note="ASELLLPRAAS -> TSWVSEEEISV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_013929"
FT VAR_SEQ 518..619
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_013930"
FT CONFLICT 71..73
FT /note="NPK -> SYQ (in Ref. 4; BAC98038)"
FT /evidence="ECO:0000305"
FT CONFLICT 156
FT /note="H -> R (in Ref. 2; EDL24000, 3; AAI18935 and 4;
FT BAC98038)"
FT /evidence="ECO:0000305"
FT CONFLICT 321
FT /note="R -> H (in Ref. 2; EDL24000 and 3; AAI18935)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 619 AA; 69572 MW; 2D1201F6CB8CA28E CRC64;
MELTQKEREL SRWAAEEMEV PLAARPREST LRRLCLSQGA DIWAYIVQHV RSQRNIKKIQ
GNLLWHAYQD NPKIHRKLEL EATVARLRAE NQELDQSLEL MDQESEAQDV AMTQTLQSLK
DTQHRALLLQ AQAGAVRRQQ RGLQDPMQRL QNQLKHLQDM QRKAKVDVTF GPVVSAAPAL
EPEVLGDVRA ACTLRTQFLQ NLLTPRARGG SILSPCDDHV GTSYQQWLTS VETLLTNHPA
GHVLAALEYL AAERESEIRS LCYGDGLKEE ELSRPQAPES SNSSQVLPST VHLIQEGWQA
VGALVTQRSA LLSERQVLTG RLQRLVEEVK RLLLGSSERK VLLLGLRHSG LLAELKALHA
QSQELESAVG QRHLLLRELQ AKRQRILQWR QLVEDRQEQI RLLIKGNSAS KTRLSRGPEE
VLALIDQKLV PTSEAVAPQS QELLRCLKEE AKHLPRVLLG PLLPYHVKGL KPLSRILPSI
HQLHPTNPRA SSLILLSHTL GLPVGKASEL LLPRAASLQQ DLLFLQDQLG LRRGNLCVKT
SLPPGPSTQE LLQMQVSQEK EQNENVGQTL KKLSNLLKQA LEQIPELQGI VQDWWEQPSQ
AALPEEICQG LSLPQCQLR
