HAUS8_MOUSE
ID HAUS8_MOUSE Reviewed; 373 AA.
AC Q99L00; Q9CWT4;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=HAUS augmin-like complex subunit 8;
DE AltName: Full=HEC1/NDC80-interacting centrosome-associated protein 1;
DE AltName: Full=Sarcoma antigen NY-SAR-48 homolog;
GN Name=Haus8; Synonyms=Hice1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Contributes to mitotic spindle assembly, maintenance of
CC centrosome integrity and completion of cytokinesis as part of the HAUS
CC augmin-like complex. {ECO:0000250}.
CC -!- SUBUNIT: Component of the HAUS augmin-like complex. The complex
CC interacts with the gamma-tubulin ring complex and this interaction is
CC required for spindle assembly. Associates with microtubules. The
CC interaction with microtubules is strong during mitosis, while it is
CC weak or absent during interphase. It is unclear whether this
CC interaction is direct or indirect (By similarity). Interacts with EML3
CC (phosphorylated at 'Thr-882') and TUBG1 (By similarity).
CC {ECO:0000250|UniProtKB:Q9BT25}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BT25}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC {ECO:0000250|UniProtKB:Q9BT25}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q9BT25}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000250|UniProtKB:Q9BT25}. Note=During interphase, primarily
CC cytoplasmic and associates with centrosomes and with the mitotic
CC spindles, preferentially at the spindle pole vicinity. During anaphase
CC and telophase, additionally associates with the spindle midzone and
CC midbody, respectively. Localizes to mitotic spindle microtubules (By
CC similarity). {ECO:0000250|UniProtKB:Q9BT25}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q99L00-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q99L00-2; Sequence=VSP_031544;
CC -!- SIMILARITY: Belongs to the HAUS8 family. {ECO:0000305}.
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DR EMBL; AK010398; BAB26909.1; -; mRNA.
DR EMBL; BC003935; AAH03935.1; -; mRNA.
DR CCDS; CCDS22389.1; -. [Q99L00-2]
DR CCDS; CCDS52579.1; -. [Q99L00-1]
DR RefSeq; NP_001156514.1; NM_001163042.1. [Q99L00-1]
DR RefSeq; NP_083897.2; NM_029621.3. [Q99L00-2]
DR AlphaFoldDB; Q99L00; -.
DR SMR; Q99L00; -.
DR BioGRID; 218144; 7.
DR IntAct; Q99L00; 5.
DR STRING; 10090.ENSMUSP00000040802; -.
DR iPTMnet; Q99L00; -.
DR PhosphoSitePlus; Q99L00; -.
DR EPD; Q99L00; -.
DR jPOST; Q99L00; -.
DR MaxQB; Q99L00; -.
DR PaxDb; Q99L00; -.
DR PeptideAtlas; Q99L00; -.
DR PRIDE; Q99L00; -.
DR ProteomicsDB; 269765; -. [Q99L00-1]
DR ProteomicsDB; 269766; -. [Q99L00-2]
DR Antibodypedia; 27511; 207 antibodies from 25 providers.
DR DNASU; 76478; -.
DR Ensembl; ENSMUST00000035960; ENSMUSP00000040802; ENSMUSG00000035439. [Q99L00-1]
DR Ensembl; ENSMUST00000110071; ENSMUSP00000105698; ENSMUSG00000035439. [Q99L00-2]
DR GeneID; 76478; -.
DR KEGG; mmu:76478; -.
DR UCSC; uc009mcg.2; mouse. [Q99L00-2]
DR UCSC; uc009mch.2; mouse. [Q99L00-1]
DR CTD; 93323; -.
DR MGI; MGI:1923728; Haus8.
DR VEuPathDB; HostDB:ENSMUSG00000035439; -.
DR eggNOG; ENOG502S04A; Eukaryota.
DR GeneTree; ENSGT00390000010974; -.
DR HOGENOM; CLU_060977_0_0_1; -.
DR InParanoid; Q99L00; -.
DR OMA; TAKMEHN; -.
DR OrthoDB; 809178at2759; -.
DR PhylomeDB; Q99L00; -.
DR TreeFam; TF332998; -.
DR Reactome; R-MMU-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-MMU-380259; Loss of Nlp from mitotic centrosomes.
DR Reactome; R-MMU-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-MMU-380284; Loss of proteins required for interphase microtubule organization from the centrosome.
DR Reactome; R-MMU-380320; Recruitment of NuMA to mitotic centrosomes.
DR Reactome; R-MMU-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-MMU-8854518; AURKA Activation by TPX2.
DR BioGRID-ORCS; 76478; 14 hits in 72 CRISPR screens.
DR ChiTaRS; Haus8; mouse.
DR PRO; PR:Q99L00; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q99L00; protein.
DR Bgee; ENSMUSG00000035439; Expressed in granulocyte and 192 other tissues.
DR ExpressionAtlas; Q99L00; baseline and differential.
DR Genevisible; Q99L00; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0070652; C:HAUS complex; ISS:UniProtKB.
DR GO; GO:1990498; C:mitotic spindle microtubule; ISS:UniProtKB.
DR GO; GO:0005880; C:nuclear microtubule; IBA:GO_Central.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007098; P:centrosome cycle; ISS:UniProtKB.
DR GO; GO:0051225; P:spindle assembly; ISS:UniProtKB.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cell cycle; Cell division; Coiled coil;
KW Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Phosphoprotein;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9BT25"
FT CHAIN 2..373
FT /note="HAUS augmin-like complex subunit 8"
FT /id="PRO_0000319938"
FT REGION 1..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 130..204
FT /evidence="ECO:0000255"
FT COMPBIAS 28..68
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9BT25"
FT MOD_RES 99
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BT25"
FT VAR_SEQ 71
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_031544"
FT CONFLICT 50
FT /note="G -> V (in Ref. 2; AAH03935)"
FT /evidence="ECO:0000305"
FT CONFLICT 259
FT /note="Q -> R (in Ref. 1; BAB26909)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 373 AA; 41653 MW; 9F9896D057BD885E CRC64;
MADSSERDAG KSAAAGACAV PKTKGRRVQG RRVVESRYLQ YDKKAKKVSG AAKEEKPPEG
RKASTVPRSR EESQVMGTGN LQSTMLEGHG MNPPDLDLSA IDDKILSRKA SWPDREMTDK
AKSTSFISCD KKRILRKKRR DLQETMDMME SQTLLMTLLS VKMENNLALL EERAEKDLAA
MCHEKERLQR QALELRRQLL LRQKHQELAA TLDAQIEVLS PLQPVLERFK EEYMTLGRAL
DTTRHELPMQ AVHMEGSGQE LLDDLEPALR ITLQLLGDLS ICSPYATAQV QGASAQEPGA
STQLSCLLKE LKGLVTEKDL ELRRLVSQVV ELSSQASKEA ALMNQEVWEE AEGALTSSQW
YFSPDACRDD SPS
