AN13B_MOUSE
ID AN13B_MOUSE Reviewed; 626 AA.
AC Q5F259; B9EKW8; Q6IR40;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Ankyrin repeat domain-containing protein 13B;
GN Name=Ankrd13b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 268-626 (ISOFORM 2).
RC STRAIN=129; TISSUE=Brain, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Ubiquitin-binding protein that specifically recognizes and
CC binds 'Lys-63'-linked ubiquitin. Does not bind 'Lys-48'-linked
CC ubiquitin. Positively regulates the internalization of ligand-activated
CC EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell
CC membrane (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with EGFR (ubiquitinated); the interaction is direct
CC and may regulate EGFR internalization. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane. Late endosome. Early endosome.
CC Note=Interaction with EGFR may enhance association with the cell
CC membrane. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5F259-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5F259-2; Sequence=VSP_019405;
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DR EMBL; AL607072; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC071189; AAH71189.1; ALT_SEQ; mRNA.
DR EMBL; BC151158; AAI51159.1; -; mRNA.
DR CCDS; CCDS36233.1; -. [Q5F259-2]
DR CCDS; CCDS88195.1; -. [Q5F259-1]
DR RefSeq; NP_766533.2; NM_172945.2. [Q5F259-2]
DR RefSeq; XP_006533458.1; XM_006533395.2.
DR AlphaFoldDB; Q5F259; -.
DR SMR; Q5F259; -.
DR BioGRID; 234498; 1.
DR STRING; 10090.ENSMUSP00000090568; -.
DR iPTMnet; Q5F259; -.
DR PhosphoSitePlus; Q5F259; -.
DR MaxQB; Q5F259; -.
DR PaxDb; Q5F259; -.
DR PRIDE; Q5F259; -.
DR ProteomicsDB; 296354; -. [Q5F259-1]
DR ProteomicsDB; 296355; -. [Q5F259-2]
DR Antibodypedia; 26768; 59 antibodies from 18 providers.
DR Ensembl; ENSMUST00000037593; ENSMUSP00000073584; ENSMUSG00000037907. [Q5F259-1]
DR Ensembl; ENSMUST00000092892; ENSMUSP00000090568; ENSMUSG00000037907. [Q5F259-2]
DR GeneID; 268445; -.
DR KEGG; mmu:268445; -.
DR UCSC; uc007kgw.1; mouse. [Q5F259-2]
DR UCSC; uc011xzw.1; mouse. [Q5F259-1]
DR CTD; 124930; -.
DR MGI; MGI:2144501; Ankrd13b.
DR VEuPathDB; HostDB:ENSMUSG00000037907; -.
DR eggNOG; KOG0522; Eukaryota.
DR GeneTree; ENSGT00950000182928; -.
DR HOGENOM; CLU_026137_2_0_1; -.
DR InParanoid; Q5F259; -.
DR OMA; GYMKKIF; -.
DR OrthoDB; 425969at2759; -.
DR PhylomeDB; Q5F259; -.
DR TreeFam; TF314176; -.
DR BioGRID-ORCS; 268445; 3 hits in 77 CRISPR screens.
DR ChiTaRS; Ankrd13b; mouse.
DR PRO; PR:Q5F259; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5F259; protein.
DR Bgee; ENSMUSG00000037907; Expressed in embryonic brain and 194 other tissues.
DR ExpressionAtlas; Q5F259; baseline and differential.
DR Genevisible; Q5F259; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005770; C:late endosome; ISS:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0140036; F:ubiquitin-dependent protein binding; ISS:UniProtKB.
DR GO; GO:0002091; P:negative regulation of receptor internalization; ISS:UniProtKB.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR021832; ANKRD13.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR003903; UIM_dom.
DR PANTHER; PTHR12447; PTHR12447; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF11904; GPCR_chapero_1; 1.
DR SMART; SM00248; ANK; 3.
DR SMART; SM00726; UIM; 2.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
DR PROSITE; PS50330; UIM; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Alternative splicing; ANK repeat; Cell membrane; Endosome;
KW Membrane; Reference proteome; Repeat.
FT CHAIN 1..626
FT /note="Ankyrin repeat domain-containing protein 13B"
FT /id="PRO_0000240644"
FT REPEAT 47..76
FT /note="ANK 1"
FT REPEAT 80..109
FT /note="ANK 2"
FT DOMAIN 503..522
FT /note="UIM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00213"
FT DOMAIN 585..604
FT /note="UIM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00213"
FT DOMAIN 610..626
FT /note="UIM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00213"
FT REGION 442..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 534..614
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 449..470
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 556..575
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 597..614
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q86YJ7"
FT VAR_SEQ 552..626
FT /note="SAPPTPQRQPMPPAPVPSPRPSPGPGSSSHVFRSYDEQLRLAMELSAQEQEE
FT RRRRVRQEEEELERILRLSLTEQ -> PREPSVPSTPGIENPLSRWLLPTPFPGTGRVA
FT SAPGMCRDQGPSSLDLQTLNNRQVSQVCRERGALDKSSPPPGAPHPRRSASPCPRLQCP
FT ALGPAQVPAPAAMCSGATTSSCGWLWSCPLRSRRRGGGECARRRKSWSGSCGSR (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019405"
SQ SEQUENCE 626 AA; 70374 MW; 7C37F62EF2B33A55 CRC64;
MIPANASARK GPEGKYPLHY LVWHNRHREL EKEVRAGQVD IEQLDPRGRT PLHLATTLGH
LECARVLLAH GADVGRENRS GWTVLQEAVS TRDLELVQLV LRYRDYQRVV KRLAGIPMLL
EKLRKAQDFY VEMKWEFTSW VPLVSKICPS DTYKVWKSGQ NLRVDTTLLG FDHMTWQRGN
RSFVFRGQDT SAVVMEIDHD RRVVYMETLA LAGQDRELLL AAAQPSEEQV LSRLTAPVVT
TQLDTKNISF ERNKTGILGW RSEKTEMVNG YEAKVYGASN VELITRTRTE HLSEQHKGKV
KGCKTPLQSF LGIAEQHGGP QNGTLITQTL SQANPPAITA EEYFNPNFEL GNRAMGRPME
LTTKTQKFKA KLWLCEEHPL SLCEQVAPII DLMAVSNALF AKLRDFITLR LPPGFPVKIE
IPIFHILNAR ITFGNLNGCD EPVPSVRGSP GSETPSPGSD SSSVSSSSST TSCRACEISP
ALFEAPRGYS VLGGQREAVP RDEDDDLLRF AIQQSLLEAG SEYDQVTIWE ALTNSKPGTH
PMSYEGRRQD RSAPPTPQRQ PMPPAPVPSP RPSPGPGSSS HVFRSYDEQL RLAMELSAQE
QEERRRRVRQ EEEELERILR LSLTEQ
