AN34A_HUMAN
ID AN34A_HUMAN Reviewed; 496 AA.
AC Q69YU3; B3KSU3;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Ankyrin repeat domain-containing protein 34A;
GN Name=ANKRD34A; Synonyms=ANKRD34;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Cerebellum, and Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 343-496.
RC TISSUE=Amygdala;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP METHYLATION AT GLN-15, AND MUTAGENESIS OF GLN-15.
RX PubMed=26797129; DOI=10.1074/jbc.m115.711952;
RA Kusevic D., Kudithipudi S., Jeltsch A.;
RT "Substrate specificity of the HEMK2 protein glutamine methyltransferase and
RT identification of novel substrates.";
RL J. Biol. Chem. 291:6124-6133(2016).
CC -!- PTM: Methylated at Gln-15 by N6AMT1. {ECO:0000269|PubMed:26797129}.
CC -!- SIMILARITY: Belongs to the ANKRD34 family. {ECO:0000305}.
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DR EMBL; AK094282; BAG52855.1; -; mRNA.
DR EMBL; AK128050; BAG54622.1; -; mRNA.
DR EMBL; AL160282; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL359622; CAH10676.1; -; mRNA.
DR CCDS; CCDS72874.1; -.
DR RefSeq; NP_001034977.1; NM_001039888.3.
DR AlphaFoldDB; Q69YU3; -.
DR SMR; Q69YU3; -.
DR BioGRID; 129917; 47.
DR IntAct; Q69YU3; 1.
DR STRING; 9606.ENSP00000475189; -.
DR iPTMnet; Q69YU3; -.
DR PhosphoSitePlus; Q69YU3; -.
DR BioMuta; ANKRD34A; -.
DR DMDM; 172045718; -.
DR jPOST; Q69YU3; -.
DR MassIVE; Q69YU3; -.
DR PaxDb; Q69YU3; -.
DR PeptideAtlas; Q69YU3; -.
DR PRIDE; Q69YU3; -.
DR ProteomicsDB; 66169; -.
DR Antibodypedia; 75647; 16 antibodies from 7 providers.
DR DNASU; 284615; -.
DR Ensembl; ENST00000606888.3; ENSP00000475189.1; ENSG00000272031.3.
DR GeneID; 284615; -.
DR KEGG; hsa:284615; -.
DR MANE-Select; ENST00000606888.3; ENSP00000475189.1; NM_001039888.4; NP_001034977.1.
DR UCSC; uc021ouy.3; human.
DR CTD; 284615; -.
DR DisGeNET; 284615; -.
DR GeneCards; ANKRD34A; -.
DR HGNC; HGNC:27639; ANKRD34A.
DR HPA; ENSG00000272031; Tissue enhanced (brain, skeletal muscle).
DR neXtProt; NX_Q69YU3; -.
DR OpenTargets; ENSG00000272031; -.
DR PharmGKB; PA162376563; -.
DR VEuPathDB; HostDB:ENSG00000272031; -.
DR eggNOG; ENOG502QT4A; Eukaryota.
DR GeneTree; ENSGT00390000012355; -.
DR HOGENOM; CLU_042805_0_0_1; -.
DR InParanoid; Q69YU3; -.
DR OMA; PGNLCPD; -.
DR OrthoDB; 466691at2759; -.
DR PhylomeDB; Q69YU3; -.
DR TreeFam; TF331155; -.
DR PathwayCommons; Q69YU3; -.
DR SignaLink; Q69YU3; -.
DR BioGRID-ORCS; 284615; 7 hits in 1061 CRISPR screens.
DR GenomeRNAi; 284615; -.
DR Pharos; Q69YU3; Tdark.
DR PRO; PR:Q69YU3; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q69YU3; protein.
DR Bgee; ENSG00000272031; Expressed in prefrontal cortex and 106 other tissues.
DR ExpressionAtlas; Q69YU3; baseline and differential.
DR Genevisible; Q69YU3; HS.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR042637; AN34A/B/C.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR PANTHER; PTHR24156; PTHR24156; 1.
DR Pfam; PF12796; Ank_2; 1.
DR SMART; SM00248; ANK; 4.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 2.
PE 1: Evidence at protein level;
KW ANK repeat; Methylation; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..496
FT /note="Ankyrin repeat domain-containing protein 34A"
FT /id="PRO_0000319100"
FT REPEAT 4..33
FT /note="ANK 1"
FT REPEAT 37..72
FT /note="ANK 2"
FT REPEAT 76..106
FT /note="ANK 3"
FT REPEAT 110..139
FT /note="ANK 4"
FT REGION 147..473
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..163
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 202..218
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 219..235
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 376..390
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 427..458
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 15
FT /note="N5-methylglutamine"
FT /evidence="ECO:0000269|PubMed:26797129"
FT MOD_RES 316
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q5BJT1"
FT MUTAGEN 15
FT /note="Q->R: Abolishes methylation by N6AMT1."
FT /evidence="ECO:0000269|PubMed:26797129"
SQ SEQUENCE 496 AA; 52636 MW; F13B4F866CD60AC2 CRC64;
MLHTEGHALL RAVGQGKLRL ARLLLEGGAY VNEGDAQGET ALMAACRARY DDPQNKARMV
RYLLEQGADP NIADRLGRTA LMHACAGGGG AAVASLLLAH GADPSVRDHA GASALVHALD
RGDRETLATL LDACKAKGTE VIIITTDTSP SGTKKTRQYL NSPPSPGVED PAPASPSPGF
CTSPSEIQLQ TAGGGGRGML SPRAQEEEEK RDVFEFPLPK PPDDPSPSEP LPKPPRHPPK
PLKRLNSEPW GLVAPPQPVP PTEGRPGIER LTAEFNGLTL TGRPRLSRRH STEGPEDPPP
WAEKVTSGGP LSRRNTAPEA QESGPPSGLR QKLSRMEPVE LDTPGHLCPD SPESSRLSLE
RRRYSASPLT LPPAGSAPSP RQSQESLPGA VSPLSGRRRS PGLLERRGSG TLLLDHISQT
RPGFLPPLNV SPHPPIPDIR PQPGGRAPSL PAPPYAGAPG SPRTKRKLVR RHSMQTEQIR
LLGGFQSLGG PGEPGR
