ID   HBB_POTCF               Reviewed;          43 AA.
AC   P0DUJ9;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=Antimicrobial protein PcfHb {ECO:0000303|PubMed:22683678};
DE   Flags: Fragments;
OS   Potamotrygon cf. henlei (Freshwater stingray).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
OC   Elasmobranchii; Batoidea; Myliobatiformes; Potamotrygonidae; Potamotrygon.
OX   NCBI_TaxID=2805830;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RX   PubMed=22683678; DOI=10.1016/j.toxicon.2012.05.025;
RA   Conceicao K., Monteiro-dos-Santos J., Seibert C.S., Silva P.I. Jr.,
RA   Marques E.E., Richardson M., Lopes-Ferreira M.;
RT   "Potamotrygon cf. henlei stingray mucus: biochemical features of a novel
RT   antimicrobial protein.";
RL   Toxicon 60:821-829(2012).
CC   -!- FUNCTION: Shows antimicrobial activity against M.luteus (MIC=4 uM) and
CC       E.coli (MIC=12 uM), as well as against the yeast C.tropicalis (MIC=4
CC       uM) (PubMed:22683678). Shows a pro-inflammatory effect, since the
CC       topical application of the protein induces an increase of cellular
CC       recruitment characterized by an increase in the number of leukocyte
CC       rolling (PubMed:22683678). Does not show hemolytic activity on human
CC       erythrocytes (at doses up to 100 uM) (PubMed:22683678).
CC       {ECO:0000269|PubMed:22683678}.
CC   -!- SUBUNIT: Possible monomer. {ECO:0000305|PubMed:22683678}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22683678}.
CC       Note=Secreted in cutaneous mucus. {ECO:0000269|PubMed:22683678}.
CC   -!- TISSUE SPECIFICITY: Expressed in mucus-secreting tissues.
CC       {ECO:0000305|PubMed:22683678}.
CC   -!- MASS SPECTROMETRY: Mass=16072.8; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:22683678};
CC   -!- SIMILARITY: Belongs to the globin family. {ECO:0000305}.
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DR   AlphaFoldDB; P0DUJ9; -.
DR   SMR; P0DUJ9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide; Immunity;
KW   Innate immunity; Secreted.
FT   CHAIN           1..43
FT                   /note="Antimicrobial protein PcfHb"
FT                   /evidence="ECO:0000305|PubMed:22683678"
FT                   /id="PRO_0000452542"
FT   NON_CONS        32..33
FT                   /evidence="ECO:0000305|PubMed:22683678"
SQ   SEQUENCE   43 AA;  4970 MW;  B268CE3A03A687B7 CRC64;
     GKLTDSQEDY IRHVWDDVNR KLITAKALER VNLVAEALSS NYH