ID   ANAN_STRCD              Reviewed;          17 AA.
AC   Q7M0J9;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Anantin;
DE   AltName: Full=GC-A blocker;
DE   AltName: Full=NPR-A antagonist;
OS   Streptomyces coerulescens.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=29304;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=1826288; DOI=10.7164/antibiotics.44.172;
RA   Wyss D.F., Lahm H.-W., Manneberg M., Labhardt A.M.;
RT   "Anantin -- a peptide antagonist of the atrial natriuretic factor (ANF).
RT   II. Determination of the primary sequence by NMR on the basis of proton
RT   assignments.";
RL   J. Antibiot. 44:172-180(1991).
RN   [2]
RP   CHARACTERIZATION, AND MASS SPECTROMETRY.
RX   PubMed=1849131; DOI=10.7164/antibiotics.44.164;
RA   Weber W., Fischli W., Hochuli E., Kupfer E., Weibel E.K.;
RT   "Anantin -- a peptide antagonist of the atrial natriuretic factor (ANF). I.
RT   Producing organism, fermentation, isolation and biological activity.";
RL   J. Antibiot. 44:164-171(1991).
RN   [3]
RP   FUNCTION.
RX   PubMed=9486150; DOI=10.1152/ajpendo.1998.274.2.e218;
RA   Rotem R., Zamir N., Keynan N., Barkan D., Breitbart H., Naor Z.;
RT   "Atrial natriuretic peptide induces acrosomal exocytosis of human
RT   spermatozoa.";
RL   Am. J. Physiol. 274:E218-E223(1998).
RN   [4]
RP   FUNCTION.
RX   PubMed=11259543;
RA   Carvajal J.A., Aguan K., Thompson L.P., Buhimschi I.A., Weiner C.P.;
RT   "Natriuretic peptide-induced relaxation of myometrium from the pregnant
RT   guinea pig is not mediated by guanylate cyclase activation.";
RL   J. Pharmacol. Exp. Ther. 297:181-188(2001).
RN   [5]
RP   FUNCTION.
RX   PubMed=14634036; DOI=10.1124/jpet.103.060913;
RA   Moro C., Galitzky J., Sengenes C., Crampes F., Lafontan M., Berlan M.;
RT   "Functional and pharmacological characterization of the natriuretic
RT   peptide-dependent lipolytic pathway in human fat cells.";
RL   J. Pharmacol. Exp. Ther. 308:984-992(2004).
CC   -!- FUNCTION: Competitive antagonist of atrial natriuretic peptide NPR-A
CC       receptors. It binds competitively to atrial natriuretic peptide (ANP)
CC       receptors from bovine adrenal cortex and inhibits the ANP-induced
CC       intracellular cGMP accumulation in bovine aorta smooth muscle cells.
CC       Can also reduce the increase in cGMP produced by ANP or BNP (brain
CC       natriuretic peptide) in pregnant guinea pig myometrium, but has no
CC       effect on relaxation induced by either peptide. Blocks the ANP-induced
CC       human sperm acrosome reaction without affecting the acrosomal
CC       exocytosis or sperm motility. Also exhibits major and deleterious
CC       nonspecific (and even cytotoxic) effects on human fat cells, as it
CC       displays noncompetitive antagonism and exerts an inhibitory action on
CC       basal and beta-adrenergic receptor-induced lipolytic response.
CC       {ECO:0000269|PubMed:11259543, ECO:0000269|PubMed:14634036,
CC       ECO:0000269|PubMed:9486150}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=1869.9; Method=FAB;
CC       Evidence={ECO:0000269|PubMed:1849131};
CC   -!- MISCELLANEOUS: Anantin has been tested for antimicrobial activity
CC       against a broad variety of bacteria and fungi, but no antibiotic
CC       activity has been found.
CC   -!- MISCELLANEOUS: Inhibits the binding of ANP by 50% (IC(50)) at 1.0 uM.
CC   -!- CAUTION: The isopeptide linked residue 8 is shown as Asn rather than
CC       Asp as mentioned in PubMed:1826288, because it is not known whether Asp
CC       or Asn is encoded and the isopeptide bonds are almost always formed
CC       between the amides Asn or Gln and N6-lysine or alpha amino groups, with
CC       the liberation of an ammonia that makes the reaction essentially
CC       irreversible. {ECO:0000305}.
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DR   PIR; A61211; A61211.
DR   AlphaFoldDB; Q7M0J9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Isopeptide bond; Secreted.
FT   PEPTIDE         1..17
FT                   /note="Anantin"
FT                   /id="PRO_0000044106"
FT   CROSSLNK        1..8
FT                   /note="Isoaspartyl glycine isopeptide (Gly-Asn)"
SQ   SEQUENCE   17 AA;  1888 MW;  094752C473CD2902 CRC64;
     GFIGWGNNIF GHYSGDF