ID   HBD_CLODI               Reviewed;         281 AA.
AC   P45364;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=3-hydroxybutyryl-CoA dehydrogenase;
DE            EC=1.1.1.157;
DE   AltName: Full=Beta-hydroxybutyryl-CoA dehydrogenase;
DE            Short=BHBD;
GN   Name=hbd;
OS   Clostridioides difficile (Peptoclostridium difficile).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Clostridioides.
OX   NCBI_TaxID=1496;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=E;
RX   PubMed=8001771; DOI=10.1111/j.1574-6968.1994.tb07262.x;
RA   Mullany P., Clayton C.L., Pallen M.J., Slone R., Al-Saleh A.,
RA   Tabaqchali S.;
RT   "Genes encoding homologues of three consecutive enzymes in the
RT   butyrate/butanol-producing pathway of Clostridium acetobutylicum are
RT   clustered on the Clostridium difficile chromosome.";
RL   FEMS Microbiol. Lett. 124:61-67(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3S)-3-hydroxybutanoyl-CoA + NADP(+) = acetoacetyl-CoA + H(+)
CC         + NADPH; Xref=Rhea:RHEA:16197, ChEBI:CHEBI:15378, ChEBI:CHEBI:57286,
CC         ChEBI:CHEBI:57316, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.157;
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism.
CC   -!- SIMILARITY: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; X79899; CAA56272.1; -; Genomic_DNA.
DR   PIR; I40679; I40679.
DR   AlphaFoldDB; P45364; -.
DR   SMR; P45364; -.
DR   PRIDE; P45364; -.
DR   UniPathway; UPA00863; -.
DR   GO; GO:0008691; F:3-hydroxybutyryl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR022694; 3-OHacyl-CoA_DH.
DR   InterPro; IPR006180; 3-OHacyl-CoA_DH_CS.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF00725; 3HCDH; 1.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   PIRSF; PIRSF000105; HCDH; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00067; 3HCDH; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; NADP; Oxidoreductase.
FT   CHAIN           1..281
FT                   /note="3-hydroxybutyryl-CoA dehydrogenase"
FT                   /id="PRO_0000109258"
FT   SITE            137
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   281 AA;  30668 MW;  192357C9B050E98C CRC64;
     MKLAVIGSGT MGSGIVQTFA SCGHDVCLKS RTQGAIDKCL ALLDKNLTKL VTKGKWMKAT
     KAEILSHVSS TTNYEDLKDM DLIIEASVED MNIKKDVFKL LDELCKEDTI LATNTSSLSI
     TEIASSTKRP DKVIGMHFFN PVPMMKLVEV ISGQLTSKVT FDTVFELSKS INKVPVDVSE
     SPGFVVNRIL IPMINEAVGI YADGVASKEE IDEAMKLGAN HPMGPLALGD LIGLDVVLAI
     MNVLYTEFGD TKYTAHPLLA KMVRANQLGR KTKIGFYDYN K