ANA_DROME
ID ANA_DROME Reviewed; 474 AA.
AC Q26307; Q9V524;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Protein anachronism;
DE Flags: Precursor;
GN Name=ana; ORFNames=CG8084;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Eye imaginal disk;
RX PubMed=7916657; DOI=10.1016/0092-8674(93)90291-w;
RA Ebens A.J., Garren H., Cheyette B.N.R., Zipursky S.L.;
RT "The Drosophila anachronism locus: a glycoprotein secreted by glia inhibits
RT neuroblast proliferation.";
RL Cell 74:15-27(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Negatively regulates proliferation of neuronal precursor
CC cells, thereby controlling the timing of postembryonic neurogenesis.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Synthesized in some glial cells and secreted.
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DR EMBL; S63815; AAB27582.1; -; mRNA.
DR EMBL; AE013599; AAF58998.1; -; Genomic_DNA.
DR RefSeq; NP_477038.1; NM_057690.3.
DR AlphaFoldDB; Q26307; -.
DR BioGRID; 61751; 2.
DR DIP; DIP-21780N; -.
DR IntAct; Q26307; 4.
DR STRING; 7227.FBpp0289728; -.
DR GlyGen; Q26307; 6 sites.
DR PaxDb; Q26307; -.
DR DNASU; 35913; -.
DR EnsemblMetazoa; FBtr0088559; FBpp0087642; FBgn0011746.
DR GeneID; 35913; -.
DR KEGG; dme:Dmel_CG8084; -.
DR UCSC; CG8084-RA; d. melanogaster.
DR CTD; 35913; -.
DR FlyBase; FBgn0011746; ana.
DR VEuPathDB; VectorBase:FBgn0011746; -.
DR eggNOG; ENOG502SAYS; Eukaryota.
DR HOGENOM; CLU_575240_0_0_1; -.
DR InParanoid; Q26307; -.
DR PhylomeDB; Q26307; -.
DR BioGRID-ORCS; 35913; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35913; -.
DR PRO; PR:Q26307; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0011746; Expressed in oviduct (Drosophila) and 18 other tissues.
DR ExpressionAtlas; Q26307; baseline and differential.
DR Genevisible; Q26307; DM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007406; P:negative regulation of neuroblast proliferation; IMP:FlyBase.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Glycoprotein; Neurogenesis;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..474
FT /note="Protein anachronism"
FT /id="PRO_0000020730"
FT REGION 173..195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 359..474
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 359..377
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 439..474
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 54
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 62
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 73
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 116
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 144
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 342
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 388
FT /note="A -> G (in Ref. 1; AAB27582)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 474 AA; 54015 MW; FE8A43042BB595A1 CRC64;
MASAMRGEKC ERSRIRELVL ILSLITMAGD SRATPFDPSF FIEGVQSEVV NPFNRTILNR
FNLTEEQILS IQNRSNPNMR DDSAQSSNQQ YLQQVATQRL NDIFKRVQKA ISNEPNGSAS
KEKAGFPICN AETTNPEDWS LGNNVTLQFA SSVFISNNDD RLSSALLRLY KTNPGQTREH
NPGQASTQPI STENPGNTAP NCAEQPPVGP QIRVTVSIVH QQRKKQRKKR TCNTAMLSSS
STGWVEIDVK CALAYWEQQH RQQLRQQQPL QPQLTASVVG ILMIEVHDDE ENLLRPGLYF
APPTCDQADI AVPWSVYRTE PFKSHLASWT LPRKPRLDIF FNGSNSMKNS YNTPKSRAFI
ESTTSNSPTI DNQLDESGEY ESQQRVHAPL VHHRRHHHNH QQPESESESA QLEAEIEAEE
LMSSASNSEQ QMEPISNHHR HRTGHHHPHH QLHQHHHHHH RHTKHHRIPA HKQE
