ANC1_CAEEL
ID ANC1_CAEEL Reviewed; 8545 AA.
AC Q9N4M4; O61841; O61842; Q6IMP3;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2003, sequence version 3.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Nuclear anchorage protein 1;
DE Short=Anchorage 1 protein;
DE AltName: Full=Nesprin homolog;
GN Name=anc-1 {ECO:0000312|WormBase:ZK973.6};
GN ORFNames=ZK973.6 {ECO:0000312|WormBase:ZK973.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-1751, IDENTIFICATION OF COMPLETE SEQUENCE,
RP FUNCTION, INTERACTION WITH F-ACTIN AND UNC-84, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Bristol N2;
RX PubMed=12169658; DOI=10.1126/science.1075119;
RA Starr D.A., Han M.;
RT "Role of ANC-1 in tethering nuclei to the actin cytoskeleton.";
RL Science 298:406-409(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 689-1243.
RX PubMed=12408964; DOI=10.1006/geno.2002.6859;
RA Zhang Q., Ragnauth C., Greener M.J., Shanahan C.M., Roberts R.G.;
RT "The nesprins are giant actin-binding proteins, orthologous to Drosophila
RT melanogaster muscle protein MSP-300.";
RL Genomics 80:473-481(2002).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=6889924; DOI=10.1016/0092-8674(82)90038-1;
RA Hedgecock E.M., Thomson J.N.;
RT "A gene required for nuclear and mitochondrial attachment in the nematode
RT Caenorhabditis elegans.";
RL Cell 30:321-330(1982).
RN [5]
RP FUNCTION.
RX PubMed=10375507; DOI=10.1242/dev.126.14.3171;
RA Malone C.J., Fixsen W.D., Horvitz H.R., Han M.;
RT "UNC-84 localizes to the nuclear envelope and is required for nuclear
RT migration and anchoring during C. elegans development.";
RL Development 126:3171-3181(1999).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15773756;
RA Starr D.A., Han M.;
RT "A genetic approach to study the role of nuclear envelope components in
RT nuclear positioning.";
RL Novartis Found. Symp. 264:208-219(2005).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22298703; DOI=10.1534/genetics.111.136184;
RA Johnson R.P., Kramer J.M.;
RT "Neural maintenance roles for the matrix receptor dystroglycan and the
RT nuclear anchorage complex in Caenorhabditis elegans.";
RL Genetics 190:1365-1377(2012).
CC -!- FUNCTION: Plays a central role in nuclear and mitochondrial anchoring
CC (PubMed:12169658, PubMed:6889924, PubMed:10375507, PubMed:22298703).
CC Probably connects nuclei to the cytoskeleton by interacting with unc-84
CC at the nuclear envelope and with F-actin in the cytoplasm, creating a
CC bridge across the nuclear envelope between the cytoskeleton and the
CC nucleus (PubMed:15773756). Has a role in positioning of the cell body
CC of the PVQ lumbar interneuron (PubMed:22298703).
CC {ECO:0000269|PubMed:10375507, ECO:0000269|PubMed:12169658,
CC ECO:0000269|PubMed:22298703, ECO:0000269|PubMed:6889924,
CC ECO:0000303|PubMed:15773756}.
CC -!- SUBUNIT: Interacts with F-actin via its N-terminal domain. Most likely
CC interacts with unc-84; the interaction is probably required to recruit
CC anc-1 to the nuclear envelope. {ECO:0000269|PubMed:12169658}.
CC -!- SUBCELLULAR LOCATION: Nucleus outer membrane
CC {ECO:0000269|PubMed:15773756, ECO:0000305|PubMed:12169658}; Single-pass
CC type IV membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC Cytoplasm, cytoskeleton {ECO:0000305|PubMed:15773756}. Note=The largest
CC part of the protein is cytoplasmic, while its C-terminal part is
CC associated either with the nuclear envelope, most probably the outer
CC nuclear membrane, or with mitochondrial membrane.
CC {ECO:0000305|PubMed:12169658}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed in all postembryonic cells.
CC {ECO:0000269|PubMed:12169658}.
CC -!- DEVELOPMENTAL STAGE: First expressed in L1 larvae, and thereafter
CC throughout adulthood. {ECO:0000269|PubMed:12169658}.
CC -!- DOMAIN: The large coiled coil domains are composed of 6 nearly exact
CC repeats of 903 residues. The last repeat is partial. These repeats are
CC conserved in Hawai (CB4856), Australia (AB4) and Germany (RC301)
CC strains. The length of the repeat may be maintained because of a
CC selective advantage to keep the protein large and allow a single
CC molecule to extent more than 0,5 micrometers.
CC -!- DOMAIN: The KASH domain, which contains a potential transmembrane
CC domain, is essential for the nuclear envelope targeting.
CC -!- DISRUPTION PHENOTYPE: Nuclear anchorage is lost resulting in free-
CC floating nuclei that often cluster as the animal flexes. An increase in
CC PVQ cell bodies mispositioned to the anterior is apparent between
CC three-fold embryos and L1 larvae. Pharyngeal cells show defects
CC including mispositioned organelles. {ECO:0000269|PubMed:22298703,
CC ECO:0000269|PubMed:6889924}.
CC -!- SIMILARITY: Belongs to the nesprin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY157938; AAN35200.1; -; mRNA.
DR EMBL; BK000642; DAA04553.1; -; mRNA.
DR EMBL; BX284601; CCD73568.1; -; Genomic_DNA.
DR EMBL; AY126454; AAM95163.1; -; mRNA.
DR PIR; T33140; T33140.
DR PIR; T33141; T33141.
DR RefSeq; NP_491353.2; NM_058952.3.
DR SMR; Q9N4M4; -.
DR BioGRID; 37504; 26.
DR STRING; 6239.ZK973.6; -.
DR iPTMnet; Q9N4M4; -.
DR EPD; Q9N4M4; -.
DR PaxDb; Q9N4M4; -.
DR PeptideAtlas; Q9N4M4; -.
DR PRIDE; Q9N4M4; -.
DR EnsemblMetazoa; ZK973.6a.1; ZK973.6a.1; WBGene00000140.
DR GeneID; 172034; -.
DR UCSC; ZK973.6; c. elegans.
DR CTD; 172034; -.
DR WormBase; ZK973.6; CE33588; WBGene00000140; anc-1.
DR eggNOG; KOG0516; Eukaryota.
DR GeneTree; ENSGT00940000173830; -.
DR HOGENOM; CLU_222729_0_0_1; -.
DR InParanoid; Q9N4M4; -.
DR OMA; SDELRHN; -.
DR OrthoDB; 47at2759; -.
DR PhylomeDB; Q9N4M4; -.
DR PRO; PR:Q9N4M4; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00000140; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR ExpressionAtlas; Q9N4M4; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005635; C:nuclear envelope; IDA:WormBase.
DR GO; GO:0005640; C:nuclear outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:WormBase.
DR GO; GO:0003779; F:actin binding; ISS:WormBase.
DR GO; GO:0051087; F:chaperone binding; IEA:InterPro.
DR GO; GO:0007010; P:cytoskeleton organization; IMP:UniProtKB.
DR GO; GO:0051179; P:localization; TAS:WormBase.
DR GO; GO:0051647; P:nucleus localization; IMP:WormBase.
DR GO; GO:0006997; P:nucleus organization; IMP:WormBase.
DR GO; GO:0035046; P:pronuclear migration; IMP:WormBase.
DR CDD; cd00014; CH; 2.
DR Gene3D; 1.10.418.10; -; 2.
DR InterPro; IPR001589; Actinin_actin-bd_CS.
DR InterPro; IPR039906; Anc-1-like.
DR InterPro; IPR003103; BAG_domain.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR012315; KASH.
DR PANTHER; PTHR21524; PTHR21524; 10.
DR Pfam; PF00307; CH; 2.
DR Pfam; PF10541; KASH; 1.
DR SMART; SM00264; BAG; 6.
DR SMART; SM00033; CH; 2.
DR SMART; SM01249; KASH; 1.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS00019; ACTININ_1; 1.
DR PROSITE; PS00020; ACTININ_2; 1.
DR PROSITE; PS50021; CH; 2.
DR PROSITE; PS51049; KASH; 1.
PE 1: Evidence at protein level;
KW Actin-binding; Coiled coil; Cytoplasm; Cytoskeleton; Membrane; Nucleus;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..8545
FT /note="Nuclear anchorage protein 1"
FT /id="PRO_0000163593"
FT TOPO_DOM 1..8494
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00385"
FT TRANSMEM 8495..8513
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00385"
FT TOPO_DOM 8514..8545
FT /note="Perinuclear space"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00385"
FT DOMAIN 23..130
FT /note="Calponin-homology (CH) 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT DOMAIN 222..328
FT /note="Calponin-homology (CH) 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT REPEAT 3241..4143
FT /note="1"
FT REPEAT 4144..5097
FT /note="2"
FT REPEAT 5098..6000
FT /note="3"
FT REPEAT 6001..6903
FT /note="4"
FT REPEAT 6904..7806
FT /note="5"
FT REPEAT 7807..8199
FT /note="6"
FT DOMAIN 8486..8545
FT /note="KASH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00385"
FT REGION 1..325
FT /note="Actin-binding"
FT REGION 148..197
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3010..3033
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3241..8199
FT /note="6 X tandem repeat"
FT REGION 3913..3936
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4372..4395
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4867..4890
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 5770..5793
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 6673..6696
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 7576..7599
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 8391..8418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 8449..8480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 754..774
FT /evidence="ECO:0000255"
FT COILED 1072..1101
FT /evidence="ECO:0000255"
FT COILED 1215..1236
FT /evidence="ECO:0000255"
FT COILED 1324..1384
FT /evidence="ECO:0000255"
FT COILED 1574..1629
FT /evidence="ECO:0000255"
FT COILED 1725..1754
FT /evidence="ECO:0000255"
FT COILED 1950..1981
FT /evidence="ECO:0000255"
FT COILED 2103..2580
FT /evidence="ECO:0000255"
FT COILED 2682..2712
FT /evidence="ECO:0000255"
FT COILED 2852..2949
FT /evidence="ECO:0000255"
FT COILED 3002..3119
FT /evidence="ECO:0000255"
FT COILED 3178..3295
FT /evidence="ECO:0000255"
FT COILED 3346..3417
FT /evidence="ECO:0000255"
FT COILED 3482..3552
FT /evidence="ECO:0000255"
FT COILED 3587..3703
FT /evidence="ECO:0000255"
FT COILED 3781..3839
FT /evidence="ECO:0000255"
FT COILED 3902..4022
FT /evidence="ECO:0000255"
FT COILED 4114..4198
FT /evidence="ECO:0000255"
FT COILED 4249..4320
FT /evidence="ECO:0000255"
FT COILED 4436..4506
FT /evidence="ECO:0000255"
FT COILED 4541..4657
FT /evidence="ECO:0000255"
FT COILED 4735..4793
FT /evidence="ECO:0000255"
FT COILED 4856..4976
FT /evidence="ECO:0000255"
FT COILED 5035..5152
FT /evidence="ECO:0000255"
FT COILED 5203..5274
FT /evidence="ECO:0000255"
FT COILED 5339..5409
FT /evidence="ECO:0000255"
FT COILED 5444..5560
FT /evidence="ECO:0000255"
FT COILED 5638..5696
FT /evidence="ECO:0000255"
FT COILED 5759..5879
FT /evidence="ECO:0000255"
FT COILED 5938..6055
FT /evidence="ECO:0000255"
FT COILED 6106..6177
FT /evidence="ECO:0000255"
FT COILED 6242..6312
FT /evidence="ECO:0000255"
FT COILED 6347..6463
FT /evidence="ECO:0000255"
FT COILED 6541..6599
FT /evidence="ECO:0000255"
FT COILED 6662..6782
FT /evidence="ECO:0000255"
FT COILED 6841..6958
FT /evidence="ECO:0000255"
FT COILED 7009..7080
FT /evidence="ECO:0000255"
FT COILED 7145..7215
FT /evidence="ECO:0000255"
FT COILED 7250..7366
FT /evidence="ECO:0000255"
FT COILED 7444..7502
FT /evidence="ECO:0000255"
FT COILED 7565..7685
FT /evidence="ECO:0000255"
FT COILED 7744..7861
FT /evidence="ECO:0000255"
FT COILED 7912..7983
FT /evidence="ECO:0000255"
FT COILED 8048..8118
FT /evidence="ECO:0000255"
FT COILED 8153..8204
FT /evidence="ECO:0000255"
FT COILED 8273..8329
FT /evidence="ECO:0000255"
FT COILED 8370..8390
FT /evidence="ECO:0000255"
FT COMPBIAS 148..193
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 7849
FT /note="K -> N (in Ref. 1; DAA04553)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 8545 AA; 956482 MW; 02A94D994BEE19E3 CRC64;
MSSSPPARPC CVCFRFRPHE DEKAQKNTFT RWINFHLEEH SSSGRIEDLF EDIRDGVLLC
HLIEVLTGEA LAVHKGRVSK RVHHIANLTT ALTVLRRRGL ELINNNAADI ADGNPRIVLG
LIWQIILHFQ IETNMILLRE WGWAATGTEE PTSSAQPEVV VTAPSPTPSS KKSHSKVSSL
SGSKTSLASG EKAPSSPLRQ RIASFLTPTK KAPKLTAQPV KQSVEQVFLR WINAEIGDLV
GGRRVENMDK QWRDGILFCA LVSRWRPDVI SMREVTNANP RDNLELAFNL AHQHLGVRRL
LAVEDMMIEK PDKRSVITYV SQFVRMFGER SPMQGREQHE IFLAWLEATY LLCTRHELNS
QECSRIRREF IEHRPLFNTI IVTKVNYDVE ELVEIEKKWD CIRETLEKYA RRSERDLPEP
FASIATWIAG AEHILSRPLD LDATDAKKTV TMLQKLISEH QKYMEDLPKR QEDFEEAVKH
GGLGGRPVAP EFSEPLRARF AQIEEENEPR ISTLRILTTH YILLQYLQHI DEKIILWRTA
DSVTLLLRWI KEYTQLNAEN PQAKCASYIN KITLTMANDQ NSKLDKEAIL NTSNEKTAET
LKRFESLWIE LKLLKVEWVE WETHVSQLEE IIEERRRNGV PPTPEDEQAL AVITAGADQL
APKLGASARL SNNQRLDVLT HSFKKLTKTT IKIGGRLVVE LEPSTSEQAS KISYSWQASD
ELLKVEMQLR DRIQRDADSS DREQLEYRAE TFRNIRDKLI ELERLNEIYD NHSRDQVDTP
NRNLIRHDMG TIIYGLQAGQ YANFVDLSCY AFVYDEYNQV PVNLTENVLS EDYVREIVNR
KKAILTRREN NEDEIRESIR DMEECDRIIE GWQSTEIEEL RAEWNIKLSE FESWHEMMQQ
VEVLSQTIQT RLDVTVIQTI WILKERSYDI KHSELGGTLR ESLEQLATTS ETSVNRHLQN
LELSNEQDCP DAIEFLEEVG RESVSKLSAA VDDRYIYTLH VLRTKMELFR RLQNFCDAVK
ILRSQNTKWN GIKISQIDQV QSEIDTLIVR LDEEWTQDAN QLRAELASIH GSFFQLEFDR
LNEKLNMLIH DKDKLRELMV HRRHYLTAAN ELITDSKTDL AQRATSSDHP DDILRATDEV
TKALDIKGEE LRRLGELAEM NITDLVVVAL ILSFRRRQLG SEGEPEVEEL RRALREIIAR
PITEPRDVSP DAIVADILRM KDEKKRDEKT IDEIQATTLT DEQRASFAPL IEDYRRRADR
HRIVFEHLVM IYLDWLSRQF DELEDEIGMT IQTSRADDLR RMNSTEWNKW KTDLANIERQ
VGPDTKKALS AELADLHRKR DSMEARINKY LTHSAKINAK LTQFEKWLNA IEEDIEQTER
QFEDPERSYR FGSLHEVALA KQRLVAKLER LNVANKEEVL RLCERYHTIM HKLTPFQTAV
GLPLHVSTNL DRNGPFQSQI SVSSIASSEL ERPESVMSLT SSIGVIPADV AELSPFEAKI
NKLLQKLHII EDSYLKGPKP IDTVREDVKQ LEKYRNRGAE ILQQLSTSNI EDAEKEGLKH
RFVLMLNNYD DILRSIENEI RDDNELTAKN QEILAELSNA EQTLQNSPLE DLDISAELDR
LQMQLDLVKV MCNKPRKYVE CELIDSSREG SPQERRRRKK KVMVMVSNTI TTIIHVVEER
LEASDISRNP DVQQKLVAVK ENLRELDTTT VTPHPPSIMS PLGTENRNDL EEVKRLAAEI
DRAIDTASSM YEDAPTDEDA LKSAIHLLDD QKVTLNHLHV VLEGIPEKNE QDRTDAIDIA
SSVGEKLGNL KSAVEEVYEE VLASTNPVKE DQPLQHIQEV QTTPAEQSNW DTDEFSRQPP
VLSDERIELK TDPSAIDKFE VRSDEDPVPK IIDLFGQLQT AVNEASPLAC EGTDDVDALQ
VASDKLTKQD RTIRKIHAIL DTIDDPVQKP AIIESLDKIK DQINNARDNI NRQIDNLNYN
QTPVVVPKES TKTPLNEIED AVRQASTVVS DELCNTEKLL SARQTLANVK PQVDSVEANV
WSNPETIETV TPILEEYTTL VDNIEKKLAN EIEVPTNDPS RQDLVQQLQD VILECEEVVV
NCDNIEKLEE SKLKLEKARP LLDQIGDNVE KLSREQSPDT SDAIDALSNV HQQYNATIMS
IDDKIDELKN PEEDTSAADQ LISELHVISE MPAVTIDLSM LNAIEEGLST LPAHQAENVQ
AKIDELRQKK EVADQTEQIL SDLNAFGDMP AITLDLDLLK SVEDGIAVLP VEDSERIKAK
IVDLRKKKED ADQAEALLQE LSVISDMPIS TLDLNMLQGI EDNLNSLPAE ESDEIREKLN
ELRRRKQESD QAEALLQELS VISDMPISTL DLNMLQGVED NLNSLPTEEA DKIREKINDL
RRRKQESDLA EALLQELSVI YDMPTSTIDL NMLQGIEDNL NSLPAEESDK IREKINDLRR
RKQESDLAEA LLQELSVIYD MPTSTIDLNM LQGIEDNLNS LPAEESDKIR EKINDLRRRK
QESDQAEALL QELSVVSDMP ASTIDINMLQ SIEDGLSTLL SEDRSKIQQA IDSLRKKKSD
SDLAQHALEA LSVQSKLPSV SINLEELKKL EETLSTVPVE DSKVIRDKIA ELKTEKALAD
HAENYLVELK KIEDMPISAV GSDVLATIED QILQMPVQYQ PSVKETLDKL KQAKEEDDKL
AGVYDELEKI AKLPARDYDN KLLAKIDEKL NSLPKDQIAE THRKVEDIKV TKADIVAQID
VLDKLPAKDI DEHLLNSIEE KLPTIPSDSS DQLQIAIGKL RDRKQANIDE GKKILNELAE
IQKMPADSLN EHALNLLATE SDKFGSEISD KIMQEIDVLR EKQNNHEVAR LNAESVLQQL
DKISEEPHLS LTEERLAPFL QNIDTVPACF VDKIRNKINE VQKLHDEAVQ DEKDELKEKL
VAKVQNIGKT SIDDVNVSDF EEIEREINGS LEAFEAEPLL AKIQELREAK RVGDEARSAA
HDQIVALEKE AEDVTAKESA KKKKKDKKKS PQEMIDELSA KVVEAKALIP KIEEAAKNEN
LPADDKPKAE QLVSNLEAFV KDVETQVSEK QDELDKLNNA NDAIKRLGDA LDDAEKTVVP
SSVPALSEFK DRIAPHLATL VEAVNDVPAS VEPSAVALRD RAAKFVSDLE KNIQKTGDDE
KRADELKNDV GNAVKNVEDV VSKYQNQPQP LDVAKDDANK LKATVEQLTK LAESSDKIDP
QVAKDIKDSK TKAKELLQAL EKAIPQEDAI RREQAEINDR LNNLEKELTK VDEFKPEDAL
PIVDQLAANT NTLKTATDSN NEKAVAPSSL ISHDDLVVGL PEKVFQLQHA IDDKKQALNK
AAAVNEIAPK LQLVSQQLQS VPQEVPASLD EQKQLLEDVE NQKHNLENLL ANLPENDPTA
DELRQKSQWD LSRLKDLLKQ LGSAVGDKLA ALAAFNAARK NAEDALLDIT REDGGDDNKS
PDELIDDLAK KEETVAKLLD TVSGVKPDEL DDKERAEYND LLARLATAAD VLKNKRAELE
QAVKAKADEK SLHDSVDRIV SRLVPLVRES DELRHNAEAV PTQYAPKAEE LKKEVEAAKA
VIANAPSSDA HVQQLEQAVA TAETLIPDLE ERARLWNEFL AARNDIDALI EQLQQPLDAV
LAQPKRSAEE AAQDVENLRN NSQQLSDLDN KIANLQRISE LLDPLESAYA DVRFFDVDAE
QTRHQYDDVL NDVAAELEDE TLLKQSASQV ANEIDDISKM IDSTDPERSI LDTIAKSDIP
ALKAQINRIK DRIVNADASR KHVTTDPKIA EDLDNKLAKL QTELDDAIKT SDEHDKEQLI
LSLKLNISQF EQIPLDQLKS DDLKTAEKEI TNSLKPEEAE PLLAKIQELR EAKRVGDEAR
SAAHDQIVAL EKEAEDVTAK ESAKKKKKDK KKSPQEMIDE LSAKVVEAKA LIPKIEEAAK
NENLPADDKP KAEQLVSNLE AFVKDVETQV SEKQDELDKL NNANDAIKRL GDALDDAEKT
VVPSSVPALS EFKDRIAPHL ATLVEAVNDV PASVEPSAVA LRDRAAKFVS DLEKNIQKTG
DDEKRADELK NDDGNAVKNV EDVVSKYQNQ PQPLDVAKDD ANKLKATVEQ LTKLAESSDK
IDPQVAKDIK DSKTKAKELL QALEKAIPQE DAIRREQAEI NDRLNNLEKE LTKVDEFKPE
DALPIVDQLA ANTNTLKTAT DSNNEKAVAP SSLISHDDLV VGLPEKVFQL QHAIDDKKQA
LNKAAAVNEI APKLQLVSQQ LQSVPQEVPA SLDEQKQLLE DVENQKHNLE NLLANLPEND
PTADELRQKS QWDLSRLKDL LKQLGSAVGD KLAALAAFNA ARKNAEDALL DITREDGGDD
NKSPDELIDD RGRSTGSAVG DKLAALAAFN AARKNAEDAL LDITREDGGD DNKSPDELID
DLAKKEETVA KLLDTVSGVK PDELDDKERA EYNDLLARLA TAADVLKNKR AELEQAVKAK
ADEKSLHDSV DRIVSRLVPL VRESDELRHN AEAVPTQYAP KAEELKKEVE AAKAVIANAP
SSDAHVQQLE QAVATAETLI PDLEERARLW NEFLAARNDI DALIEQLQQP LDAVLAQPKR
SAEEAAQDVE NLRNNSQQLS DLDNKIANLQ RISELLDPLE SAYADVRFFD VDAEQTRHQY
DDVLNDVAAE LEDETLLKQS ASQVANEIDD ISKMIDSTDP ERSILDTIAK SDIPALKAQI
NRIKDRIVNA DASRKHVTTD PKIAEDLDNK LAKLQTELDD AIKTSDEHDK EQLILSLKLN
ISQFEQIPLD QLKSDDLKTA EKEITNSLKP EEAEPLLAKI QELREAKRVG DEARSAAHDQ
IVALEKEAED VTAKESAKKK KKDKKKSPQE MIDELSAKVV EAKALIPKIE EAAKNENLPA
DDKPKAEQLV SNLEAFVKDV ETQVSEKQDE LDKLNNANDA IKRLGDALDD AEKTVVPSSV
PALSEFKDRI APHLATLVEA VNDVPASVEP SAVALRDRAA KFVSDLEKNI QKTGDDEKRA
DELKNDVGNA VKNVEDVVSK YQNQPQPLDV AKDDANKLKA TVEQLTKLAE SSDKIDPQVA
KDIKDSKTKA KELLQALEKA IPQEDAIRRE QAEINDRLNN LEKELTKVDE FKPEDALPIV
DQLAANTNTL KTATDSNNEK AVAPSSLISH DDLVVGLPEK VFQLQHAIDD KKQALNKAAA
VNEIAPKLQL VSQQLQSVPQ EVPASLDEQK QLLEDVENQK HNLENLLANL PENDPTADEL
RQKSQWDLSR LKDLLKQLGS AVGDKLAALA AFNAARKNAE DALLDITRED GGDDNKSPDE
LIDDLAKKEE TVAKLLDTVS GVKPDELDDK ERAEYNDLLA RLATAADVLK NKRAELEQAV
KAKADEKSLH DSVDRIVSRL VPLVRESDEL RHNAEAVPTQ YAPKAEELKK EVEAAKAVIA
NAPSSDAHVQ QLEQAVATAE TLIPDLEERA RLWNEFLAAR NDIDALIEQL QQPLDAVLAQ
PKRSAEEAAQ DVENLRNNSQ QLSDLDNKIA NLQRISELLD PLESAYADVR FFDVDAEQTR
HQYDDVLNDV AAELEDETLL KQSASQVANE IDDISKMIDS TDPERSILDT IAKSDIPALK
AQINRIKDRI VNADASRKHV TTDPKIAEDL DNKLAKLQTE LDDAIKTSDE HDKEQLILSL
KLNISQFEQI PLDQLKSDDL KTAEKEITNS LKPEEAEPLL AKIQELREAK RVGDEARSAA
HDQIVALEKE AEDVTAKESA KKKKKDKKKS PQEMIDELSA KVVEAKALIP KIEEAAKNEN
LPADDKPKAE QLVSNLEAFV KDVETQVSEK QDELDKLNNA NDAIKRLGDA LDDAEKTVVP
SSVPALSEFK DRIAPHLATL VEAVNDVPAS VEPSAVALRD RAAKFVSDLE KNIQKTGDDE
KRADELKNDV GNAVKNVEDV VSKYQNQPQP LDVAKDDANK LKATVEQLTK LAESSDKIDP
QVAKDIKDSK TKAKELLQAL EKAIPQEDAI RREQAEINDR LNNLEKELTK VDEFKPEDAL
PIVDQLAANT NTLKTATDSN NEKAVAPSSL ISHDDLVVGL PEKVFQLQHA IDDKKQALNK
AAAVNEIAPK LQLVSQQLQS VPQEVPASLD EQKQLLEDVE NQKHNLENLL ANLPENDPTA
DELRQKSQWD LSRLKDLLKQ LGSAVGDKLA ALAAFNAARK NAEDALLDIT REDGGDDNKS
PDELIDDLAK KEETVAKLLD TVSGVKPDEL DDKERAEYND LLARLATAAD VLKNKRAELE
QAVKAKADEK SLHDSVDRIV SRLVPLVRES DELRHNAEAV PTQYAPKAEE LKKEVEAAKA
VIANAPSSDA HVQQLEQAVA TAETLIPDLE ERARLWNEFL AARNDIDALI EQLQQPLDAV
LAQPKRSAEE AAQDVENLRN NSQQLSDLDN KIANLQRISE LLDPLESAYA DVRFFDVDAE
QTRHQYDDVL NDVAAELEDE TLLKQSASQV ANEIDDISKM IDSTDPERSI LDTIAKSDIP
ALKAQINRIK DRIVNADASR KHVTTDPKIA EDLDNKLAKL QTELDDAIKT SDEHDKEQLI
LSLKLNISQF EQIPLDQLKS DDLKTAEKEI TNSLKPEEAE PLLAKIQELR EAKRVGDEAR
SAAHDQIVAL EKEAEDVTAK ESAKKKKKDK KKSPQEMIDE LSAKVVEAKA LIPKIEEAAK
NENLPADDKP KAEQLVSNLE AFVKDVETQV SEKQDELDKL NNANDAIKRL GDALDDAEKT
VVPSSVPALS EFKDRIAPHL ATLVEAVNDV PASVEPSAVA LRDRAAKFVS DLEKNIQKTG
DDEKRADELK NDVGNAVKNV EDVVSKYQNQ PQPLDVAKDD ANKLKATVEQ LTKLAESSDK
IDPQVAKDIK DSKTKAKELL QALEKAIPQE DAIRREQAEI NDRLNNLEKE LTKVDEFKPE
DALPIVDQLA ANTNTLKTAT DSNNEKAVAP SSLISHDDLV VGLPEKVFQL QHAIDDKKQA
LNKAAAVNEI APKLQLVSQQ LQSVPQEVPA SLDEQKQLLE DVENQKHNLE NLLANLPEND
PTADELRQKS QWDLSRLKDL LKQLGSAVGD KLAALAAFNA ARKNAEDALL DITREDGGDD
NKSPDELIDD LAKKEETVAK LLDTVSGVKP DELDDKERAE YNDLLARLAT AADVLKNKRA
ELEQAVKAKA DEKSLHDSVD RIVSRLVPLV RESDELRHNA EAVPTQYAPK AEELKKEVEA
AKAVIANAPS SDAHVQQLEQ AVATAETLIP DLEERARLWN EFLAARNDID ALIEQLQQPL
DAVLAQPKRS AEEAAQDVEN LRNNSQQLSD LDNKIANLQR ISELLDPLES AYADVRFFDV
DAEQTRHQYD DVLNDVAAEL EDETLLKQSA SQVANEIDDI SKMIDSTDPE RSILDTIAKS
DIPALKAQIN RIKDRIVNAD ASRKHVTTDP KIAEDLDNKL AKLQTELDDA IKTSDEHDKE
QLILSLKLNI SQFEQIPLDQ LKSDDLKTAE KEITNSLKPE EAEPLLAKIQ ELREAKRVGD
EARSAAHDQI VALEKEAEDV TAKESAKKKK KDKKKSPQEM IDELSAKVVE AKALIPKIEE
AAKNENLPAD DKPKAEQLVS NLEAFVKDVE TQVSEKQDEL DKLNNANDAI KRLGDALDDA
EKTVVPSSVP ALSEFKDRIA PHLATLVEAV NDVPASVEPS AVALRDRAAK FVSDLEKNIQ
KTGDDEKRAD ELKNDVGNAV KNVEDVVSKY QNQPQPLDVA KDDANKLKAT VEQLTKLAES
SDKIDPQVAK DIKDSKTKAK ELLQALEKAI PQEDAIRREQ AEINDRLNKL EKELTKVDEF
KPEDALPIVD QLAANTNTLK TATDSNNEKA VAPSSLISHD DLVVGLPEKV FQLQHAIDDK
KQALNKAAAV NEIAPKLQLV SQQLQSVPQE VPASLDEQKQ LLEDVENQKH NLENLLANLP
ENDPTADELR QKSQWDLSRL KDLLKQLGSA VGEKLAALAA FNAARKNAED ALLDITREDG
GDDNKSPDEL IDDLAKKEET VAKLLDTVSG VKPDELDDKE RAEYNDLLAR LATAADVLKN
KRAELEQAVK AKADEKSLHD SVDRIVSRLV PLVRESDELR HNAEAVPTQY APKAEELKKE
VEAAKAVIAN APSSDAHVQQ LEQAVATAET LIPDLEERAS IWERFVKAKD DLYDYLEKLE
NNVSDVLNRP RLPVSQAQQR FNKLKEQSYL LDRIRDLKID FDDLGEALLP LTVAEDELRF
MHVHVESIER QYEDTMDKLN AEITAEVELL RTLDILSNEL SQCKEDINNP SVDVDELSRA
TMLNDAIAHL ENQKVVVARS EKDRKFVESS TSIDLDQLLA EAKRLLKEIE PRLQLAQPDH
DNEDDEDEEK GSDEKPYDVR AAAEVLSALY PDEHPHNVLR NIGFEELPSD SESRSEFDSL
DSRSDGLLSP IPDDSTLSEE QLRRQRSRWR RVLRTALPLQ ALLVLLMGAA CLVPHCDDEY
CCQLLNNFAK SFDPSLEFVN GPPPF
