HBD_THETC
ID HBD_THETC Reviewed; 281 AA.
AC P77851; D9TPZ7; O08502;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=3-hydroxybutyryl-CoA dehydrogenase;
DE EC=1.1.1.157;
DE AltName: Full=Beta-hydroxybutyryl-CoA dehydrogenase;
DE Short=BHBD;
GN Name=hbd; OrderedLocusNames=Tthe_1657;
OS Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 /
OS NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) (Clostridium
OS thermosaccharolyticum).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacterales Family III. Incertae Sedis; Thermoanaerobacterium.
OX NCBI_TaxID=580327;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM
RC 00135 / 2032;
RA van Rinsum A., Bronnenmeier K., Staudenbauer W.;
RL Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM
RC 00135 / 2032;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA Pitluck S., Teshima H., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA Chang Y.-J., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N.,
RA Hemme C.L., Woyke T.;
RT "Complete sequence of Thermoanaerobacterium thermosaccharolyticum DSM
RT 571.";
RL Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3S)-3-hydroxybutanoyl-CoA + NADP(+) = acetoacetyl-CoA + H(+)
CC + NADPH; Xref=Rhea:RHEA:16197, ChEBI:CHEBI:15378, ChEBI:CHEBI:57286,
CC ChEBI:CHEBI:57316, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.1.1.157;
CC -!- PATHWAY: Lipid metabolism; butanoate metabolism.
CC -!- SIMILARITY: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
CC {ECO:0000305}.
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DR EMBL; Z82038; CAB04792.1; -; Genomic_DNA.
DR EMBL; Z92974; CAB07499.1; -; Genomic_DNA.
DR EMBL; CP002171; ADL69166.1; -; Genomic_DNA.
DR PIR; T45289; T45289.
DR RefSeq; WP_013298133.1; NC_014410.1.
DR AlphaFoldDB; P77851; -.
DR SMR; P77851; -.
DR STRING; 580327.Tthe_1657; -.
DR PRIDE; P77851; -.
DR EnsemblBacteria; ADL69166; ADL69166; Tthe_1657.
DR KEGG; ttm:Tthe_1657; -.
DR eggNOG; COG1250; Bacteria.
DR HOGENOM; CLU_009834_2_0_9; -.
DR OMA; SVMEVFY; -.
DR OrthoDB; 862072at2; -.
DR UniPathway; UPA00863; -.
DR Proteomes; UP000001626; Chromosome.
DR GO; GO:0008691; F:3-hydroxybutyryl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.1040.10; -; 1.
DR InterPro; IPR022694; 3-OHacyl-CoA_DH.
DR InterPro; IPR006180; 3-OHacyl-CoA_DH_CS.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR013328; 6PGD_dom2.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR PIRSF; PIRSF000105; HCDH; 1.
DR SUPFAM; SSF48179; SSF48179; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00067; 3HCDH; 1.
PE 3: Inferred from homology;
KW Fatty acid metabolism; Lipid metabolism; NADP; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..281
FT /note="3-hydroxybutyryl-CoA dehydrogenase"
FT /id="PRO_0000109260"
FT SITE 138
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250"
FT CONFLICT 47..53
FT /note="NLQRNVD -> IYKEMLT (in Ref. 1; CAB04792/CAB07499)"
FT /evidence="ECO:0000305"
FT CONFLICT 57..64
FT /note="ITADEKNE -> LQQMRKR (in Ref. 1; CAB04792/CAB07499)"
FT /evidence="ECO:0000305"
FT CONFLICT 204..211
FT /note="DGVATAED -> VVLATDKS (in Ref. 1; CAB04792/CAB07499)"
FT /evidence="ECO:0000305"
FT CONFLICT 231
FT /note="D -> S (in Ref. 1; CAB04792/CAB07499)"
FT /evidence="ECO:0000305"
FT CONFLICT 263
FT /note="M -> V (in Ref. 1; CAB04792/CAB07499)"
FT /evidence="ECO:0000305"
FT CONFLICT 281
FT /note="K -> KINLLRRRIS (in Ref. 1; CAB04792/CAB07499)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 281 AA; 30954 MW; 8A24C291165723DC CRC64;
MQKICVIGAG TMGSGIAQVF AQNGFEVILR DIDMKFVEKG FGTIEKNLQR NVDKGKITAD
EKNEILSRIR GTTNLEDAKE ADFVVEAAIE NMDLKKQIFK ELDEICKMET ILASNTSSLS
ITEIASATKR PEKVIGMHFF NPVPVMKLVE VIKGLKTSEQ TFNVVRELAL KVDKTPIEVK
EAPGFVVNRI LIPMINEAIG ILADGVATAE DIDEAMKLGA NHPIGPLALS DLIGNDVVLA
IMNVLYEEYG DSKYRPHPLL KKMVRGGLLG RKTGKGFFEY K
