ID   HBEAG_WHV2              Reviewed;         217 AA.
AC   P0C6J3;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=External core antigen {ECO:0000255|HAMAP-Rule:MF_04076};
DE   AltName: Full=HBeAg {ECO:0000255|HAMAP-Rule:MF_04076};
DE   AltName: Full=Precore protein {ECO:0000255|HAMAP-Rule:MF_04076};
DE   AltName: Full=p25 {ECO:0000255|HAMAP-Rule:MF_04076};
DE   Flags: Precursor;
GN   Name=C {ECO:0000255|HAMAP-Rule:MF_04076};
OS   Woodchuck hepatitis B virus (isolate 2) (WHV).
OC   Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC   Blubervirales; Hepadnaviridae; Orthohepadnavirus.
OX   NCBI_TaxID=341946;
OH   NCBI_TaxID=9995; Marmota monax (Woodchuck).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3855246; DOI=10.1128/jvi.56.3.978-986.1985;
RA   Kodama K., Ogasawara N., Yoshikawa H., Murakami S.;
RT   "Nucleotide sequence of a cloned woodchuck hepatitis virus genome:
RT   evolutional relationship between hepadnaviruses.";
RL   J. Virol. 56:978-986(1985).
CC   -!- FUNCTION: May regulate immune response to the intracellular capsid in
CC       acting as a T-cell tolerogen, by having an immunoregulatory effect
CC       which prevents destruction of infected cells by cytotoxic T-cells. This
CC       immune regulation may predispose to chronicity during perinatal
CC       infections and prevent severe liver injury during adult infections.
CC       {ECO:0000255|HAMAP-Rule:MF_04076}.
CC   -!- SUBUNIT: Homodimerizes. {ECO:0000255|HAMAP-Rule:MF_04076}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|HAMAP-Rule:MF_04076}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04076}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=External core antigen;
CC         IsoId=P0C6J3-1; Sequence=Displayed;
CC       Name=Capsid protein;
CC         IsoId=P06433-1; Sequence=External;
CC   -!- PTM: Phosphorylated. {ECO:0000255|HAMAP-Rule:MF_04076}.
CC   -!- PTM: Cleaved by host furin. {ECO:0000255|HAMAP-Rule:MF_04076}.
CC   -!- SIMILARITY: Belongs to the orthohepadnavirus precore antigen family.
CC       {ECO:0000255|HAMAP-Rule:MF_04076}.
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DR   EMBL; M11082; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR   SMR; P0C6J3; -.
DR   Proteomes; UP000007632; Genome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039619; C:T=4 icosahedral viral capsid; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0005198; F:structural molecule activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0075521; P:microtubule-dependent intracellular transport of viral material towards nucleus; IEA:UniProtKB-UniRule.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-UniRule.
DR   GO; GO:0075732; P:viral penetration into host nucleus; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.4090.10; -; 1.
DR   HAMAP; MF_04076; HBV_HBEAG; 1.
DR   InterPro; IPR013195; Hepatitis_B_virus_capsid_N.
DR   InterPro; IPR002006; Hepatitis_core.
DR   InterPro; IPR036459; Viral_capsid_core_dom_sf_HBV.
DR   Pfam; PF08290; Hep_core_N; 1.
DR   Pfam; PF00906; Hepatitis_core; 3.
DR   SUPFAM; SSF47852; SSF47852; 1.
PE   3: Inferred from homology;
KW   Alternative initiation; Disulfide bond; Host nucleus;
KW   Host-virus interaction; Repeat; Secreted; Signal; Viral immunoevasion.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04076"
FT   CHAIN           20..217
FT                   /note="External core antigen"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04076"
FT                   /id="PRO_0000324742"
FT   PROPEP          189..217
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000324743"
FT   REPEAT          189..195
FT                   /note="1; half-length"
FT   REPEAT          196..203
FT                   /note="2"
FT   REPEAT          204..211
FT                   /note="3"
FT   REGION          26..28
FT                   /note="HBEAG"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04076"
FT   REGION          180..217
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          189..211
FT                   /note="3 X 8 AA repeats of S-P-R-R-R-R-S-Q"
FT   COMPBIAS        185..207
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            188..189
FT                   /note="Cleavage; by host"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04076"
FT   DISULFID        78
FT                   /note="Interchain"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04076"
FT   DISULFID        91
FT                   /note="Interchain"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04076"
SQ   SEQUENCE   217 AA;  25010 MW;  A5A3040F2DDB5FD2 CRC64;
     MYLFHLCLVF ACVPCPTFQA SKLCLGWLWG MDIDPYKEFG SSYQLLNFLP LDFFPDLNAL
     VDTATALYEE ELTGREHCSP HHTAIRQALV CWDELTKLIA WMSSNITSEQ VRTIIVNHVN
     DTWGLKVRQS LWFHLSCLTF GQHTVQEFLV SFVVWIRTPA PYRPPNAPIL STLPEHTVIR
     RGGARASRSP RRRTPSPRRR RSQSPRRRRS QSPSANC