HBL1_ORYSJ
ID HBL1_ORYSJ Reviewed; 166 AA.
AC O04986; Q10PH5; Q7G715;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Non-symbiotic hemoglobin 1;
DE AltName: Full=ORYsa GLB1a;
DE AltName: Full=rHb1;
GN Name=HB1; Synonyms=GLB1A; OrderedLocusNames=Os03g0233900, LOC_Os03g13140;
GN ORFNames=OJ1175C11.5, OsJ_10047 {ECO:0000312|EMBL:EAZ26180.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], CHARACTERIZATION, AND MUTAGENESIS
RP OF HIS-74.
RC STRAIN=cv. Jackson;
RX PubMed=9390447; DOI=10.1104/pp.115.3.1259;
RA Arredondo-Peter R., Hargrove M.S., Sarath G., Moran J.F., Lohrman J.,
RA Olson J.S., Klucas R.V.;
RT "Rice hemoglobins. Gene cloning, analysis, and O2-binding kinetics of a
RT recombinant protein synthesized in Escherichia coli.";
RL Plant Physiol. 115:1259-1266(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RA Lira-Ruan V., Ross E.J.H., Sarath G., Klucas R.V., Arredondo-Peter R.;
RT "Mapping and analysis of a hemoglobin gene family from rice (Oryza
RT sativa).";
RL Plant Physiol. Biochem. 40:199-202(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [9]
RP CHARACTERIZATION, AND MUTAGENESIS OF VAL-47; SER-50; GLU-120 AND VAL-121.
RX PubMed=11106662; DOI=10.1074/jbc.m009254200;
RA Goodman M.D., Hargrove M.S.;
RT "Quaternary structure of rice nonsymbiotic hemoglobin.";
RL J. Biol. Chem. 276:6834-6839(2001).
RN [10]
RP INDUCTION.
RC STRAIN=cv. Jackson;
RX PubMed=11448759; DOI=10.1016/s0168-9452(01)00411-3;
RA Lira-Ruan V., Sarath G., Klucas R.V., Arredondo-Peter R.;
RT "Synthesis of hemoglobins in rice (Oryza sativa var. Jackson) plants
RT growing in normal and stress conditions.";
RL Plant Sci. 161:279-287(2001).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
RX PubMed=10986467; DOI=10.1016/s0969-2126(00)00194-5;
RA Hargrove M.S., Brucker E.A., Stec B., Sarath G., Arredondo-Peter R.,
RA Klucas R.V., Olson J.S., Phillips G.N. Jr.;
RT "Crystal structure of a nonsymbiotic plant hemoglobin.";
RL Structure 8:1005-1014(2000).
CC -!- FUNCTION: May not function as an oxygen storage or transport protein,
CC but might act as an oxygen sensor or play a role in electron transfer,
CC possibly to a bound oxygen molecule. Has an unusually high affinity for
CC O(2) because of a very low dissociation constant.
CC -!- SUBUNIT: Homodimer.
CC -!- TISSUE SPECIFICITY: Expressed in coleoptiles, embryos, leaves and
CC roots.
CC -!- INDUCTION: By flooding and etiolating but not by oxidative, nitrosative
CC or hormonal stresses. {ECO:0000269|PubMed:11448759}.
CC -!- SIMILARITY: Belongs to the plant globin family. {ECO:0000305}.
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DR EMBL; U76030; AAC49883.1; -; mRNA.
DR EMBL; U76029; AAC49882.1; -; Genomic_DNA.
DR EMBL; AF335504; AAK72229.1; -; Genomic_DNA.
DR EMBL; AC103891; AAM19125.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF94821.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11390.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS83133.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ26180.1; -; Genomic_DNA.
DR EMBL; AK064054; BAG88986.1; -; mRNA.
DR PIR; T04163; T04163.
DR RefSeq; XP_015629794.1; XM_015774308.1.
DR PDB; 1D8U; X-ray; 2.35 A; A/B=1-166.
DR PDB; 2GNV; X-ray; 2.30 A; A/B=2-166.
DR PDB; 2GNW; X-ray; 2.40 A; A/B=2-166.
DR PDBsum; 1D8U; -.
DR PDBsum; 2GNV; -.
DR PDBsum; 2GNW; -.
DR AlphaFoldDB; O04986; -.
DR SMR; O04986; -.
DR STRING; 4530.OS03T0233900-01; -.
DR PaxDb; O04986; -.
DR PRIDE; O04986; -.
DR EnsemblPlants; Os03t0233900-01; Os03t0233900-01; Os03g0233900.
DR GeneID; 4332166; -.
DR Gramene; Os03t0233900-01; Os03t0233900-01; Os03g0233900.
DR KEGG; osa:4332166; -.
DR eggNOG; KOG3378; Eukaryota.
DR HOGENOM; CLU_003827_11_2_1; -.
DR InParanoid; O04986; -.
DR OMA; QRMEEAC; -.
DR OrthoDB; 1379813at2759; -.
DR BioCyc; MetaCyc:MON-19374; -.
DR EvolutionaryTrace; O04986; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; O04986; OS.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019825; F:oxygen binding; IEA:InterPro.
DR Gene3D; 1.10.490.10; -; 1.
DR InterPro; IPR000971; Globin.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012292; Globin/Proto.
DR InterPro; IPR001032; Leghaemoglobin.
DR InterPro; IPR019824; Leghaemoglobin_Fe_BS.
DR PANTHER; PTHR22924; PTHR22924; 1.
DR Pfam; PF00042; Globin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR PROSITE; PS01033; GLOBIN; 1.
DR PROSITE; PS00208; PLANT_GLOBIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Heme; Iron; Metal-binding; Reference proteome.
FT CHAIN 1..166
FT /note="Non-symbiotic hemoglobin 1"
FT /id="PRO_0000193021"
FT BINDING 74
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="distal binding residue"
FT BINDING 109
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="proximal binding residue"
FT MUTAGEN 47
FT /note="V->N: Prevents subunit association, but has little
FT effect on ligand binding."
FT /evidence="ECO:0000269|PubMed:11106662"
FT MUTAGEN 50
FT /note="S->A: Prevents subunit association, but has little
FT effect on ligand binding."
FT /evidence="ECO:0000269|PubMed:11106662"
FT MUTAGEN 74
FT /note="H->L: Increases 1000 times the dissociation constant
FT of O(2)."
FT /evidence="ECO:0000269|PubMed:9390447"
FT MUTAGEN 120
FT /note="E->V: Prevents subunit association, but has little
FT effect on ligand binding."
FT /evidence="ECO:0000269|PubMed:11106662"
FT MUTAGEN 121
FT /note="V->N: Prevents subunit association, but has little
FT effect on ligand binding."
FT /evidence="ECO:0000269|PubMed:11106662"
FT STRAND 7..9
FT /evidence="ECO:0007829|PDB:1D8U"
FT HELIX 16..29
FT /evidence="ECO:0007829|PDB:2GNV"
FT HELIX 30..32
FT /evidence="ECO:0007829|PDB:2GNV"
FT HELIX 33..47
FT /evidence="ECO:0007829|PDB:2GNV"
FT HELIX 49..53
FT /evidence="ECO:0007829|PDB:2GNV"
FT TURN 58..60
FT /evidence="ECO:0007829|PDB:2GNV"
FT HELIX 65..67
FT /evidence="ECO:0007829|PDB:2GNV"
FT HELIX 69..92
FT /evidence="ECO:0007829|PDB:2GNV"
FT HELIX 99..111
FT /evidence="ECO:0007829|PDB:2GNV"
FT HELIX 116..133
FT /evidence="ECO:0007829|PDB:2GNV"
FT TURN 136..138
FT /evidence="ECO:0007829|PDB:2GNV"
FT HELIX 141..160
FT /evidence="ECO:0007829|PDB:2GNV"
SQ SEQUENCE 166 AA; 18444 MW; C1FE2CA4701682EF CRC64;
MALVEDNNAV AVSFSEEQEA LVLKSWAILK KDSANIALRF FLKIFEVAPS ASQMFSFLRN
SDVPLEKNPK LKTHAMSVFV MTCEAAAQLR KAGKVTVRDT TLKRLGATHL KYGVGDAHFE
VVKFALLDTI KEEVPADMWS PAMKSAWSEA YDHLVAAIKQ EMKPAE
