HBL2_ORYSJ
ID HBL2_ORYSJ Reviewed; 169 AA.
AC O04985; Q10PQ0; Q7G6W7;
DT 05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Non-symbiotic hemoglobin 2;
DE AltName: Full=ORYsa GLB1b;
DE AltName: Full=rHb2;
GN Name=HB2; Synonyms=GLB1B; OrderedLocusNames=Os03g0226200, LOC_Os03g12510;
GN ORFNames=OsJ_09991 {ECO:0000312|EMBL:EAZ26128.1}, OSJNBa0081P02.19;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Jackson;
RX PubMed=9390447; DOI=10.1104/pp.115.3.1259;
RA Arredondo-Peter R., Hargrove M.S., Sarath G., Moran J.F., Lohrman J.,
RA Olson J.S., Klucas R.V.;
RT "Rice hemoglobins. Gene cloning, analysis, and O2-binding kinetics of a
RT recombinant protein synthesized in Escherichia coli.";
RL Plant Physiol. 115:1259-1266(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RA Lira-Ruan V., Ross E.J.H., Sarath G., Klucas R.V., Arredondo-Peter R.;
RT "Mapping and analysis of a hemoglobin gene family from rice (Oryza
RT sativa).";
RL Plant Physiol. Biochem. 40:199-202(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [9]
RP INDUCTION.
RC STRAIN=cv. Jackson;
RX PubMed=11448759; DOI=10.1016/s0168-9452(01)00411-3;
RA Lira-Ruan V., Sarath G., Klucas R.V., Arredondo-Peter R.;
RT "Synthesis of hemoglobins in rice (Oryza sativa var. Jackson) plants
RT growing in normal and stress conditions.";
RL Plant Sci. 161:279-287(2001).
CC -!- FUNCTION: May not function as an oxygen storage or transport protein,
CC but might act as an oxygen sensor or play a role in electron transfer,
CC possibly to a bound oxygen molecule.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, but not in roots.
CC -!- INDUCTION: By flooding and etiolating but not by oxidative, nitrosative
CC or hormonal stresses. {ECO:0000269|PubMed:11448759}.
CC -!- SIMILARITY: Belongs to the plant globin family. {ECO:0000305}.
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DR EMBL; U76028; AAC49881.1; -; Genomic_DNA.
DR EMBL; U76031; AAC49884.1; -; mRNA.
DR EMBL; AF335503; AAK72228.1; -; Genomic_DNA.
DR EMBL; AC107226; AAN52754.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF94743.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11351.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS83059.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ26128.1; -; Genomic_DNA.
DR EMBL; AK121522; BAH00533.1; -; mRNA.
DR PIR; T04162; T04162.
DR RefSeq; XP_015628351.1; XM_015772865.1.
DR AlphaFoldDB; O04985; -.
DR SMR; O04985; -.
DR STRING; 4530.OS03T0226200-01; -.
DR PaxDb; O04985; -.
DR PRIDE; O04985; -.
DR EnsemblPlants; Os03t0226200-01; Os03t0226200-01; Os03g0226200.
DR GeneID; 4332123; -.
DR Gramene; Os03t0226200-01; Os03t0226200-01; Os03g0226200.
DR KEGG; osa:4332123; -.
DR eggNOG; KOG3378; Eukaryota.
DR HOGENOM; CLU_003827_11_2_1; -.
DR InParanoid; O04985; -.
DR OMA; NAPPRYY; -.
DR OrthoDB; 1379813at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; O04985; OS.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019825; F:oxygen binding; IEA:InterPro.
DR Gene3D; 1.10.490.10; -; 1.
DR InterPro; IPR000971; Globin.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012292; Globin/Proto.
DR InterPro; IPR001032; Leghaemoglobin.
DR InterPro; IPR019824; Leghaemoglobin_Fe_BS.
DR PANTHER; PTHR22924; PTHR22924; 1.
DR Pfam; PF00042; Globin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR PROSITE; PS01033; GLOBIN; 1.
DR PROSITE; PS00208; PLANT_GLOBIN; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Metal-binding; Reference proteome.
FT CHAIN 1..169
FT /note="Non-symbiotic hemoglobin 2"
FT /id="PRO_0000193022"
FT BINDING 77
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="distal binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00238"
FT BINDING 112
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="proximal binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00238"
SQ SEQUENCE 169 AA; 18616 MW; 82919B002C40273B CRC64;
MALVEGNNGV SGGAVSFSEE QEALVLKSWA IMKKDSANIG LRFFLKIFEV APSASQMFSF
LRNSDVPLEK NPKLKTHAMS VFVMTCEAAA QLRKAGKVTV RDTTLKRLGA THFKYGVGDA
HFEVTRFALL ETIKEAVPVD MWSPAMKSAW SEAYNQLVAA IKQEMKPAE
