ID   HBOH_AZOSB              Reviewed;         699 AA.
AC   A1KBP4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=D-(-)-3-hydroxybutyrate oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HB-oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HBOH {ECO:0000255|HAMAP-Rule:MF_01906};
DE            EC=3.1.1.22 {ECO:0000255|HAMAP-Rule:MF_01906};
DE   Flags: Precursor;
GN   OrderedLocusNames=azo3634;
OS   Azoarcus sp. (strain BH72).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC   Azoarcus.
OX   NCBI_TaxID=418699;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BH72;
RX   PubMed=17057704; DOI=10.1038/nbt1243;
RA   Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J.,
RA   Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C.,
RA   Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J.,
RA   Weidner S., Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.;
RT   "Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp.
RT   strain BH72.";
RL   Nat. Biotechnol. 24:1385-1391(2006).
CC   -!- FUNCTION: Participates in the degradation of poly-3-hydroxybutyrate
CC       (PHB). It works downstream of poly(3-hydroxybutyrate) depolymerase,
CC       hydrolyzing D(-)-3-hydroxybutyrate oligomers of various length (3HB-
CC       oligomers) into 3HB-monomers. {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxybutanoate dimer + H2O = 2 (R)-3-hydroxybutanoate +
CC         H(+); Xref=Rhea:RHEA:10172, ChEBI:CHEBI:10979, ChEBI:CHEBI:10983,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378; EC=3.1.1.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01906};
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism. {ECO:0000255|HAMAP-
CC       Rule:MF_01906}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- SIMILARITY: Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase
CC       family. {ECO:0000255|HAMAP-Rule:MF_01906}.
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DR   EMBL; AM406670; CAL96250.1; -; Genomic_DNA.
DR   RefSeq; WP_011767356.1; NC_008702.1.
DR   AlphaFoldDB; A1KBP4; -.
DR   STRING; 62928.azo3634; -.
DR   ESTHER; azosb-hboh; OHBut_olig_hydro_put.
DR   EnsemblBacteria; CAL96250; CAL96250; azo3634.
DR   KEGG; azo:azo3634; -.
DR   eggNOG; ENOG502Z8QU; Bacteria.
DR   HOGENOM; CLU_420258_0_0_4; -.
DR   OMA; NPEKDWG; -.
DR   OrthoDB; 865015at2; -.
DR   UniPathway; UPA00863; -.
DR   Proteomes; UP000002588; Chromosome.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0047989; F:hydroxybutyrate-dimer hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01906; 3HBOH; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR016582; OHBut_olig_hydro_put.
DR   Pfam; PF10605; 3HBOH; 1.
DR   PIRSF; PIRSF011409; HObutyrate_olig_hydrol; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
FT   CHAIN           20..699
FT                   /note="D-(-)-3-hydroxybutyrate oligomer hydrolase"
FT                   /id="PRO_0000314413"
FT   ACT_SITE        303
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
SQ   SEQUENCE   699 AA;  72794 MW;  9FB5EA10603A60BF CRC64;
     MNPSLCIAVA FACPLSALAA SGAPGVGFNE KPSFLGEIVQ RSVDGVSDDL LTAGLGRDGI
     QSATPPLVSA SPTATELRRL AIYNNYRALV DTTSGGGFGS LFGPLIAVDA GGRVSAPGDG
     KIAGTEYLAY AIDRGDDSKV TLMVQVPAHF NPKAPCIVTG TSSGSRGIYG AIGTSGEWGL
     QKGCAVAYAD KGTGNGMHDL DTNTVGLIDG TRADAVAAGV ASHFTADLTE AERTQFLASW
     PHRVAVKHAH SRANPEAHWG QDTLDAVRFA FFVLNETYGR RTPRGIVRTI LPPNTVVIAS
     SVSNGGGAAL AAAEQDDERL IDGVAVGEPQ IQLRENDAVR VVRGSQVRTG TGRPLYDYTT
     FANLLQPCAV LSPRAAGSPG EAFIPAALAA NRCEALAAHE FITGNTPAER GDSALDALVA
     YGWEPESTPL TASHYAFAVP PIALTYANAY GRFGVEERVC NYSFAATDAA GTPIAWPAAS
     AATSFGTGNG IPPAAGLQII NDASLGGPRR DGASISPSTG KLDFNVDGAL CLRQLWTGGG
     ASAREVHESV DEVQVSAQLQ GRPAIIVHGR ADALVPVGFT SRPYLGLNSL AEHGRSRLRY
     VEVTNAQHFD AFIGTATLPG YDTRFVPLHV YFRQALDLMY SHLTARTPLP PSQLVRTTPR
     AGTPGAAVAI TAANVPPIRM KPAAADLITV RRGEVVVPD