ID   HBOH_BURCH              Reviewed;         696 AA.
AC   A0K516;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=D-(-)-3-hydroxybutyrate oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HB-oligomer hydrolase {ECO:0000255|HAMAP-Rule:MF_01906};
DE            Short=3HBOH {ECO:0000255|HAMAP-Rule:MF_01906};
DE            EC=3.1.1.22 {ECO:0000255|HAMAP-Rule:MF_01906};
DE   Flags: Precursor;
GN   OrderedLocusNames=Bcen2424_0840;
OS   Burkholderia cenocepacia (strain HI2424).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=331272;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HI2424;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P.,
RA   Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., LiPuma J.J., Gonzalez C.F.,
RA   Konstantinidis K., Tiedje J.M., Richardson P.;
RT   "Complete sequence of chromosome 1 of Burkholderia cenocepacia HI2424.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Participates in the degradation of poly-3-hydroxybutyrate
CC       (PHB). It works downstream of poly(3-hydroxybutyrate) depolymerase,
CC       hydrolyzing D(-)-3-hydroxybutyrate oligomers of various length (3HB-
CC       oligomers) into 3HB-monomers. {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxybutanoate dimer + H2O = 2 (R)-3-hydroxybutanoate +
CC         H(+); Xref=Rhea:RHEA:10172, ChEBI:CHEBI:10979, ChEBI:CHEBI:10983,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378; EC=3.1.1.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01906};
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism. {ECO:0000255|HAMAP-
CC       Rule:MF_01906}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|HAMAP-Rule:MF_01906}.
CC   -!- SIMILARITY: Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase
CC       family. {ECO:0000255|HAMAP-Rule:MF_01906}.
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DR   EMBL; CP000458; ABK07593.1; -; Genomic_DNA.
DR   RefSeq; WP_011544713.1; NC_008542.1.
DR   AlphaFoldDB; A0K516; -.
DR   KEGG; bch:Bcen2424_0840; -.
DR   HOGENOM; CLU_420258_0_0_4; -.
DR   OMA; NPEKDWG; -.
DR   OrthoDB; 865015at2; -.
DR   UniPathway; UPA00863; -.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0047989; F:hydroxybutyrate-dimer hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01906; 3HBOH; 1.
DR   InterPro; IPR016582; OHBut_olig_hydro_put.
DR   Pfam; PF10605; 3HBOH; 1.
DR   PIRSF; PIRSF011409; HObutyrate_olig_hydrol; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
FT   CHAIN           27..696
FT                   /note="D-(-)-3-hydroxybutyrate oligomer hydrolase"
FT                   /id="PRO_5000164646"
FT   ACT_SITE        309
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01906"
SQ   SEQUENCE   696 AA;  71693 MW;  9C7DCC29A2FC4146 CRC64;
     MTKLGWGRRV VWGAALAAVA MLGACNGDES AERNRLPGFV SGSVRTTAYD GASDDLLTAG
     LGKTGLAAAT APGFANPSRP TSAELRRLAI WSNYRALVDM SANGGYGRFW GPNVDLDGND
     TLGEGKIPGT EYLAYADDGS GSKNVTLLVQ VPASFNPAQP CIVTATSSGS RGVYGAISAA
     GEWALKRGCA VAYNDKGGGN GAHELGSDTV TLIDGTLANA VLAGTASLFT ANVTSGDLAA
     FNSRFPNRYA FKHAHSQQNP EQDWGRVTLQ SVEFAYWALN EQFGPVIDGA RHGVRYRAGD
     ITTIAASVSN GGGASLAAAE QDNRGWITAV VVGEPQINVR MAPNAVVRAG GQPVPSFGRP
     LADYATLANL LEPCAAASAS LAGAPYLSAL PATTTQSIRT QRCATLAAAG LVAGADTQSQ
     AADALAQLHA AGYLADSDLL QAPMWDSQAI PAIAVTYANA YTRSRVTDNL CNFSFATTNP
     ATGVVAAPAA SPMPSVFGVG NGVPPTAGIN LVFNDGAGTD HRLATPDASF AGALCLRQLW
     TNGMLGMPAN VDAVRVNANL HGKPAIIVQG RSDALVPVNH ASRAYVAQNG ISEAGRSQLV
     FYEVTNGQHF DAFLSVPGFD TRFVPVHYYN VQALNLMWRH LKNGAPLPPS QVIRTVPRGG
     TPGAAPALTS ANLPPISTAP GANAIATGVG TIDVPL